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Metabolic profiling of human urine by CE-MS using a positively charged capillary coating and comparison with UPLC-MS.
Mol Biosyst. 2011 Jan; 7(1):194-9.MB

Abstract

The potential of capillary electrophoresis time-of-flight mass spectrometry (CE-TOF-MS) using capillaries coated with a triple layer of polybrene-dextran sulfate-polybrene (PB-DS-PB) was evaluated for metabolic profiling of human urine. The method covers various metabolite classes and stable metabolic profiles of urine samples were obtained with favourable migration time repeatability (RSDs <1%). The PB-DS-PB CE-TOF-MS method was used for the analysis of human urine samples from 30 males and 30 females, which had been previously analyzed by reversed-phase UPLC-TOF-MS. Multivariate data analysis of the obtained data provided clear distinction between urine samples from males and females, emphasizing gender differences in metabolic signatures. Nearly all compounds responsible for male-female classification in CE-TOF-MS were different from the classifying compounds in UPLC-TOF-MS. Almost all compounds causing classification in the CE-TOF-MS study were highly polar and did not exhibit retention in the reversed-phase UPLC system. In addition, the CE-TOF-MS classifiers had an m/z value in the range of 50-150, whereas 95% of the classifying features found with UPLC-TOF-MS had an m/z value above 150. The CE-TOF-MS method therefore appears to be highly complementary to the UPLC-TOF-MS method providing classification based on different classes of metabolites.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Ramautar R
Department of Biomedical Analysis, Utrecht University, Sorbonnelaan 16, PO Box 80082, 3508 TB Utrecht, The Netherlands. R.Ramautar@lumc.nl
Nevedomskaya E
No affiliation info available
Mayboroda OA
No affiliation info available
Deelder AM
No affiliation info available
Wilson ID
No affiliation info available
Gika HG
No affiliation info available
Theodoridis GA
No affiliation info available
Somsen GW
No affiliation info available
de Jong GJ
No affiliation info available

MeSH

Chromatography, LiquidElectrophoresis, CapillaryFemaleHumansMaleMass SpectrometryMetabolomeUrine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21052608

Citation

Ramautar, Rawi, et al. "Metabolic Profiling of Human Urine By CE-MS Using a Positively Charged Capillary Coating and Comparison With UPLC-MS." Molecular BioSystems, vol. 7, no. 1, 2011, pp. 194-9.
Ramautar R, Nevedomskaya E, Mayboroda OA, et al. Metabolic profiling of human urine by CE-MS using a positively charged capillary coating and comparison with UPLC-MS. Mol Biosyst. 2011;7(1):194-9.
Ramautar, R., Nevedomskaya, E., Mayboroda, O. A., Deelder, A. M., Wilson, I. D., Gika, H. G., Theodoridis, G. A., Somsen, G. W., & de Jong, G. J. (2011). Metabolic profiling of human urine by CE-MS using a positively charged capillary coating and comparison with UPLC-MS. Molecular BioSystems, 7(1), 194-9. https://doi.org/10.1039/c0mb00032a
Ramautar R, et al. Metabolic Profiling of Human Urine By CE-MS Using a Positively Charged Capillary Coating and Comparison With UPLC-MS. Mol Biosyst. 2011;7(1):194-9. PubMed PMID: 21052608.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolic profiling of human urine by CE-MS using a positively charged capillary coating and comparison with UPLC-MS. AU - Ramautar,Rawi, AU - Nevedomskaya,Ekaterina, AU - Mayboroda,Oleg A, AU - Deelder,André M, AU - Wilson,Ian D, AU - Gika,Helen G, AU - Theodoridis,Georgios A, AU - Somsen,Govert W, AU - de Jong,Gerhardus J, Y1 - 2010/11/05/ PY - 2010/11/6/entrez PY - 2010/11/6/pubmed PY - 2011/3/25/medline SP - 194 EP - 9 JF - Molecular bioSystems JO - Mol Biosyst VL - 7 IS - 1 N2 - The potential of capillary electrophoresis time-of-flight mass spectrometry (CE-TOF-MS) using capillaries coated with a triple layer of polybrene-dextran sulfate-polybrene (PB-DS-PB) was evaluated for metabolic profiling of human urine. The method covers various metabolite classes and stable metabolic profiles of urine samples were obtained with favourable migration time repeatability (RSDs <1%). The PB-DS-PB CE-TOF-MS method was used for the analysis of human urine samples from 30 males and 30 females, which had been previously analyzed by reversed-phase UPLC-TOF-MS. Multivariate data analysis of the obtained data provided clear distinction between urine samples from males and females, emphasizing gender differences in metabolic signatures. Nearly all compounds responsible for male-female classification in CE-TOF-MS were different from the classifying compounds in UPLC-TOF-MS. Almost all compounds causing classification in the CE-TOF-MS study were highly polar and did not exhibit retention in the reversed-phase UPLC system. In addition, the CE-TOF-MS classifiers had an m/z value in the range of 50-150, whereas 95% of the classifying features found with UPLC-TOF-MS had an m/z value above 150. The CE-TOF-MS method therefore appears to be highly complementary to the UPLC-TOF-MS method providing classification based on different classes of metabolites. SN - 1742-2051 UR - https://www.unboundmedicine.com/medline/citation/21052608/Metabolic_profiling_of_human_urine_by_CE_MS_using_a_positively_charged_capillary_coating_and_comparison_with_UPLC_MS_ L2 - https://doi.org/10.1039/c0mb00032a DB - PRIME DP - Unbound Medicine ER -