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Modulation of HLA antigen expression on conjunctival fibroblasts by gamma-interferon.
Invest Ophthalmol Vis Sci. 1990 Jan; 31(1):163-7.IO

Abstract

We investigated the ability of recombinant human gamma-interferon (rhIFN-gamma) to induce class II HLA antigen expression on human conjunctival fibroblasts in cell culture. Cultures were established by explanting subconjunctival tissue from normal donor globes. Fibroblasts were treated with rhIFN-gamma at concentrations ranging from 1 to 500 units/ml and incubated for 1, 3 and 6 days. HLA antigens were detected by immunofluorescence using monoclonal antibodies in conjunction with flow cytometry. Class I antigen was identified using a monoclonal antibody directed against Beta-2 microglobulin (a component of the class I antigen complex). Class II histocompatibility antigens were detected using monoclonal antibodies specific for HLA-DR, HLA-DP and HLA-DQ. Class I antigen was present on all cells prior to induction and showed a trend toward increased density after treatment with rhIFN-gamma. Class II antigens were absent before induction with rhIFN-gamma. After treatment with rhIFN-gamma, class II antigens were induced in a dose- and time-dependent fashion. HLA-DR expression was most sensitive to induction by rhIFN-gamma, followed by HLA-DP, and then HLA-DQ. The up-regulation of HLA class I antigen expression and the inducible expression of class II antigens following exposure to rhIFN-gamma suggest that conjunctival fibroblasts have the potential to participate in immunologic diseases of the external eye.

Authors+Show Affiliations

Jules Stein Eye Institute, Department of Ophthalmology, Los Angeles, CA 90024.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2105282

Citation

Harrison, S A., et al. "Modulation of HLA Antigen Expression On Conjunctival Fibroblasts By Gamma-interferon." Investigative Ophthalmology & Visual Science, vol. 31, no. 1, 1990, pp. 163-7.
Harrison SA, Mondino BJ, Kagan JM. Modulation of HLA antigen expression on conjunctival fibroblasts by gamma-interferon. Invest Ophthalmol Vis Sci. 1990;31(1):163-7.
Harrison, S. A., Mondino, B. J., & Kagan, J. M. (1990). Modulation of HLA antigen expression on conjunctival fibroblasts by gamma-interferon. Investigative Ophthalmology & Visual Science, 31(1), 163-7.
Harrison SA, Mondino BJ, Kagan JM. Modulation of HLA Antigen Expression On Conjunctival Fibroblasts By Gamma-interferon. Invest Ophthalmol Vis Sci. 1990;31(1):163-7. PubMed PMID: 2105282.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulation of HLA antigen expression on conjunctival fibroblasts by gamma-interferon. AU - Harrison,S A, AU - Mondino,B J, AU - Kagan,J M, PY - 1990/1/1/pubmed PY - 1990/1/1/medline PY - 1990/1/1/entrez SP - 163 EP - 7 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 31 IS - 1 N2 - We investigated the ability of recombinant human gamma-interferon (rhIFN-gamma) to induce class II HLA antigen expression on human conjunctival fibroblasts in cell culture. Cultures were established by explanting subconjunctival tissue from normal donor globes. Fibroblasts were treated with rhIFN-gamma at concentrations ranging from 1 to 500 units/ml and incubated for 1, 3 and 6 days. HLA antigens were detected by immunofluorescence using monoclonal antibodies in conjunction with flow cytometry. Class I antigen was identified using a monoclonal antibody directed against Beta-2 microglobulin (a component of the class I antigen complex). Class II histocompatibility antigens were detected using monoclonal antibodies specific for HLA-DR, HLA-DP and HLA-DQ. Class I antigen was present on all cells prior to induction and showed a trend toward increased density after treatment with rhIFN-gamma. Class II antigens were absent before induction with rhIFN-gamma. After treatment with rhIFN-gamma, class II antigens were induced in a dose- and time-dependent fashion. HLA-DR expression was most sensitive to induction by rhIFN-gamma, followed by HLA-DP, and then HLA-DQ. The up-regulation of HLA class I antigen expression and the inducible expression of class II antigens following exposure to rhIFN-gamma suggest that conjunctival fibroblasts have the potential to participate in immunologic diseases of the external eye. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/2105282/Modulation_of_HLA_antigen_expression_on_conjunctival_fibroblasts_by_gamma_interferon_ L2 - https://iovs.arvojournals.org/article.aspx?volume=31&issue=1&page=163 DB - PRIME DP - Unbound Medicine ER -