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Beneficial effects of cannabinoids (CB) in a murine model of allergen-induced airway inflammation: role of CB1/CB2 receptors.
Immunobiology 2011; 216(4):466-76I

Abstract

The endocannabinoid system (ECS) consists of two cannabinoid (CB) receptors, namely CB(1) and CB(2) receptor, and their endogenous (endocannabinoids) and exogenous (cannabinoids, e.g. delta-9-tetrahydrocannabinol (THC)) ligands which bind to these receptors. Based on studies suggesting a role of THC and the ECS in inflammation, the objective of this study was to examine their involvement in type I hypersensitivity using a murine model of allergic airway inflammation. THC treatment of C57BL/6 wildtype mice dramatically reduced airway inflammation as determined by significantly reduced total cell counts in bronchoalveolar lavage (BAL). These effects were greatest when mice were treated during both, the sensitization and the challenge phase. Furthermore, systemic immune responses were significantly suppressed in mice which received THC during sensitization phase. To investigate a role of CB(1/2) receptors in this setting, we used pharmacological blockade of CB(1) and/or CB(2) receptors by the selective antagonists and moreover CB(1)/CB(2) receptor double-knockout mice (CB(1)(-/-)/CB(2)(-/-)) and found neither significant changes in the cell patterns in BAL nor in immunoglobulin levels as compared to wildtype mice. Our results indicate that the activation of the ECS by applying the agonist THC is involved in the development of type I allergies. However, CB(1)/CB(2) receptor-independent signalling seems likely in the observed results.

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    Authors+Show Affiliations

    Braun A
    ZAUM - Center for Allergy and Environment, Division of Environmental Dermatology and Allergy, Helmholtz Zentrum München/Technische Universität München (TUM), Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany. andrea.braun@helmholtz-muenchen.de
    Engel T
    No affiliation info available
    Aguilar-Pimentel JA
    No affiliation info available
    Zimmer A
    No affiliation info available
    Jakob T
    No affiliation info available
    Behrendt H
    No affiliation info available
    Mempel M
    No affiliation info available

    MeSH

    AllergensAnimalsBronchoalveolar Lavage FluidCannabinoidsCytokinesDisease Models, AnimalFemaleImmunizationImmunoglobulinsInflammationLeukocytesMiceMice, Inbred C57BLMice, KnockoutReceptor, Cannabinoid, CB1Receptor, Cannabinoid, CB2Respiratory Tract DiseasesSpleenTh2 Cells

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    21056512

    Citation

    Braun, Andrea, et al. "Beneficial Effects of Cannabinoids (CB) in a Murine Model of Allergen-induced Airway Inflammation: Role of CB1/CB2 Receptors." Immunobiology, vol. 216, no. 4, 2011, pp. 466-76.
    Braun A, Engel T, Aguilar-Pimentel JA, et al. Beneficial effects of cannabinoids (CB) in a murine model of allergen-induced airway inflammation: role of CB1/CB2 receptors. Immunobiology. 2011;216(4):466-76.
    Braun, A., Engel, T., Aguilar-Pimentel, J. A., Zimmer, A., Jakob, T., Behrendt, H., & Mempel, M. (2011). Beneficial effects of cannabinoids (CB) in a murine model of allergen-induced airway inflammation: role of CB1/CB2 receptors. Immunobiology, 216(4), pp. 466-76. doi:10.1016/j.imbio.2010.09.004.
    Braun A, et al. Beneficial Effects of Cannabinoids (CB) in a Murine Model of Allergen-induced Airway Inflammation: Role of CB1/CB2 Receptors. Immunobiology. 2011;216(4):466-76. PubMed PMID: 21056512.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Beneficial effects of cannabinoids (CB) in a murine model of allergen-induced airway inflammation: role of CB1/CB2 receptors. AU - Braun,Andrea, AU - Engel,Tabea, AU - Aguilar-Pimentel,Juan Antonio, AU - Zimmer,Andreas, AU - Jakob,Thilo, AU - Behrendt,Heidrun, AU - Mempel,Martin, Y1 - 2010/09/18/ PY - 2010/08/12/received PY - 2010/09/13/revised PY - 2010/09/14/accepted PY - 2010/11/9/entrez PY - 2010/11/9/pubmed PY - 2011/9/29/medline SP - 466 EP - 76 JF - Immunobiology JO - Immunobiology VL - 216 IS - 4 N2 - The endocannabinoid system (ECS) consists of two cannabinoid (CB) receptors, namely CB(1) and CB(2) receptor, and their endogenous (endocannabinoids) and exogenous (cannabinoids, e.g. delta-9-tetrahydrocannabinol (THC)) ligands which bind to these receptors. Based on studies suggesting a role of THC and the ECS in inflammation, the objective of this study was to examine their involvement in type I hypersensitivity using a murine model of allergic airway inflammation. THC treatment of C57BL/6 wildtype mice dramatically reduced airway inflammation as determined by significantly reduced total cell counts in bronchoalveolar lavage (BAL). These effects were greatest when mice were treated during both, the sensitization and the challenge phase. Furthermore, systemic immune responses were significantly suppressed in mice which received THC during sensitization phase. To investigate a role of CB(1/2) receptors in this setting, we used pharmacological blockade of CB(1) and/or CB(2) receptors by the selective antagonists and moreover CB(1)/CB(2) receptor double-knockout mice (CB(1)(-/-)/CB(2)(-/-)) and found neither significant changes in the cell patterns in BAL nor in immunoglobulin levels as compared to wildtype mice. Our results indicate that the activation of the ECS by applying the agonist THC is involved in the development of type I allergies. However, CB(1)/CB(2) receptor-independent signalling seems likely in the observed results. SN - 1878-3279 UR - https://www.unboundmedicine.com/medline/citation/21056512/abstract/Beneficial_effects_of_cannabinoids__CB__in_a_murine_model_of_allergen_induced_airway_inflammation:_Role_of_CB_1_/CB_2__receptors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0171-2985(10)00159-2 DB - PRIME DP - Unbound Medicine ER -