Tags

Type your tag names separated by a space and hit enter

Upregulation of interleukin 21 and promotion of interleukin 17 production in chronic or recurrent Vogt-Koyanagi-Harada disease.
Arch Ophthalmol. 2010 Nov; 128(11):1449-54.AO

Abstract

OBJECTIVES

To analyze the expression and potential role of interleukin (IL) 21 in the pathogenesis of Vogt-Koyanagi-Harada (VKH) disease.

METHODS

Blood samples were obtained from patients with VKH disease and from healthy control subjects. Serum IL-21 level and IL-21 messenger RNA (mRNA) expression by peripheral blood mononuclear cells (PBMCs) were determined by enzyme-linked immunosorbent assay and by reverse transcriptase-polymerase chain reaction, respectively. Interleukin 17 and interferon γ levels in the supernatants of PBMCs and CD4(+) T cells cultured with anti-CD3 and anti-CD28 antibodies in the presence or absence of recombinant IL-21 were detected by enzyme-linked immunosorbent assay.

RESULTS

The results showed a significantly increased serum IL-21 level, as well as higher IL-21 mRNA expression by PBMCs, in patients having chronic or recurrent active VKH disease compared with patients having inactive VKH disease and with controls. In vitro experiments showed that recombinant IL-21 significantly increased IL-17 production by PBMCs and by CD4(+) T cells from patients and from controls. However, recombinant IL-21 did not affect interferon γ expression by PBMCs or by CD4(+) T cells.

CONCLUSION

Interleukin 21 may be involved in the pathogenesis of chronic or recurrent VKH disease, possibly by promoting IL-17 secretion.

CLINICAL RELEVANCE

Findings from the present study suggest that IL-21 may be a potential target in the development of therapy for VKH disease.

Authors+Show Affiliations

Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21060047

Citation

Li, Fuzhen, et al. "Upregulation of Interleukin 21 and Promotion of Interleukin 17 Production in Chronic or Recurrent Vogt-Koyanagi-Harada Disease." Archives of Ophthalmology (Chicago, Ill. : 1960), vol. 128, no. 11, 2010, pp. 1449-54.
Li F, Yang P, Liu X, et al. Upregulation of interleukin 21 and promotion of interleukin 17 production in chronic or recurrent Vogt-Koyanagi-Harada disease. Arch Ophthalmol. 2010;128(11):1449-54.
Li, F., Yang, P., Liu, X., Wang, C., Hou, S., & Kijlstra, A. (2010). Upregulation of interleukin 21 and promotion of interleukin 17 production in chronic or recurrent Vogt-Koyanagi-Harada disease. Archives of Ophthalmology (Chicago, Ill. : 1960), 128(11), 1449-54. https://doi.org/10.1001/archophthalmol.2010.265
Li F, et al. Upregulation of Interleukin 21 and Promotion of Interleukin 17 Production in Chronic or Recurrent Vogt-Koyanagi-Harada Disease. Arch Ophthalmol. 2010;128(11):1449-54. PubMed PMID: 21060047.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Upregulation of interleukin 21 and promotion of interleukin 17 production in chronic or recurrent Vogt-Koyanagi-Harada disease. AU - Li,Fuzhen, AU - Yang,Peizeng, AU - Liu,Xiaoli, AU - Wang,Chaokui, AU - Hou,Shengping, AU - Kijlstra,Aize, PY - 2010/11/10/entrez PY - 2010/11/10/pubmed PY - 2010/12/14/medline SP - 1449 EP - 54 JF - Archives of ophthalmology (Chicago, Ill. : 1960) JO - Arch. Ophthalmol. VL - 128 IS - 11 N2 - OBJECTIVES: To analyze the expression and potential role of interleukin (IL) 21 in the pathogenesis of Vogt-Koyanagi-Harada (VKH) disease. METHODS: Blood samples were obtained from patients with VKH disease and from healthy control subjects. Serum IL-21 level and IL-21 messenger RNA (mRNA) expression by peripheral blood mononuclear cells (PBMCs) were determined by enzyme-linked immunosorbent assay and by reverse transcriptase-polymerase chain reaction, respectively. Interleukin 17 and interferon γ levels in the supernatants of PBMCs and CD4(+) T cells cultured with anti-CD3 and anti-CD28 antibodies in the presence or absence of recombinant IL-21 were detected by enzyme-linked immunosorbent assay. RESULTS: The results showed a significantly increased serum IL-21 level, as well as higher IL-21 mRNA expression by PBMCs, in patients having chronic or recurrent active VKH disease compared with patients having inactive VKH disease and with controls. In vitro experiments showed that recombinant IL-21 significantly increased IL-17 production by PBMCs and by CD4(+) T cells from patients and from controls. However, recombinant IL-21 did not affect interferon γ expression by PBMCs or by CD4(+) T cells. CONCLUSION: Interleukin 21 may be involved in the pathogenesis of chronic or recurrent VKH disease, possibly by promoting IL-17 secretion. CLINICAL RELEVANCE: Findings from the present study suggest that IL-21 may be a potential target in the development of therapy for VKH disease. SN - 1538-3601 UR - https://www.unboundmedicine.com/medline/citation/21060047/Upregulation_of_interleukin_21_and_promotion_of_interleukin_17_production_in_chronic_or_recurrent_Vogt_Koyanagi_Harada_disease_ L2 - https://jamanetwork.com/journals/jamaophthalmology/fullarticle/10.1001/archophthalmol.2010.265 DB - PRIME DP - Unbound Medicine ER -