Tags

Type your tag names separated by a space and hit enter

Expression and RNA interference of salivary polygalacturonase genes in the tarnished plant bug, Lygus lineolaris.
J Insect Sci. 2010; 10:173.JI

Abstract

Three genes encoding polygalacturonase (PG) have been identified in Lygus lineolaris (Palisot de Beauvois) (Miridae: Hemiptera). Earlier studies showed that the three PG gene transcripts are exclusively expressed in the feeding stages of L. lineolaris. In this report, it is shown that all three transcripts are specifically expressed in salivary glands indicating that PGs are salivary enzymes. Transcriptional profiles of the three PGs were evaluated with respect to diet, comparing live cotton plant material to artificial diet. PG2 transcript levels were consistently lower in cotton-fed insects than those reared on artificial diet. RNA interference was used to knock down expression of PG1 mRNA in adult salivary glands providing the first demonstration of the use of this method in the non-model insect, L. lineolaris.

Authors+Show Affiliations

USDA-ARS, Biological Control of Pests Research Unit, 59 Lee Road, NBCL, Building 8, Stoneville, Mississippi, USA. william.walker@ARS.USDA.GOVNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

21062205

Citation

Walker, William B., and Margaret L. Allen. "Expression and RNA Interference of Salivary Polygalacturonase Genes in the Tarnished Plant Bug, Lygus Lineolaris." Journal of Insect Science (Online), vol. 10, 2010, p. 173.
Walker WB, Allen ML. Expression and RNA interference of salivary polygalacturonase genes in the tarnished plant bug, Lygus lineolaris. J Insect Sci. 2010;10:173.
Walker, W. B., & Allen, M. L. (2010). Expression and RNA interference of salivary polygalacturonase genes in the tarnished plant bug, Lygus lineolaris. Journal of Insect Science (Online), 10, 173. https://doi.org/10.1673/031.010.14133
Walker WB, Allen ML. Expression and RNA Interference of Salivary Polygalacturonase Genes in the Tarnished Plant Bug, Lygus Lineolaris. J Insect Sci. 2010;10:173. PubMed PMID: 21062205.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression and RNA interference of salivary polygalacturonase genes in the tarnished plant bug, Lygus lineolaris. AU - Walker,William B, AU - Allen,Margaret L, PY - 2010/11/11/entrez PY - 2010/11/11/pubmed PY - 2011/2/23/medline SP - 173 EP - 173 JF - Journal of insect science (Online) JO - J Insect Sci VL - 10 N2 - Three genes encoding polygalacturonase (PG) have been identified in Lygus lineolaris (Palisot de Beauvois) (Miridae: Hemiptera). Earlier studies showed that the three PG gene transcripts are exclusively expressed in the feeding stages of L. lineolaris. In this report, it is shown that all three transcripts are specifically expressed in salivary glands indicating that PGs are salivary enzymes. Transcriptional profiles of the three PGs were evaluated with respect to diet, comparing live cotton plant material to artificial diet. PG2 transcript levels were consistently lower in cotton-fed insects than those reared on artificial diet. RNA interference was used to knock down expression of PG1 mRNA in adult salivary glands providing the first demonstration of the use of this method in the non-model insect, L. lineolaris. SN - 1536-2442 UR - https://www.unboundmedicine.com/medline/citation/21062205/Expression_and_RNA_interference_of_salivary_polygalacturonase_genes_in_the_tarnished_plant_bug_Lygus_lineolaris_ L2 - https://academic.oup.com/jinsectscience/article-lookup/doi/10.1673/031.010.14133 DB - PRIME DP - Unbound Medicine ER -