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Thermal dependence of Raman descriptors of ceramides. Part II: effect of chains lengths and head group structures.
Anal Bioanal Chem. 2011 Jan; 399(3):1201-13.AB

Abstract

The outermost layer of the mammalian skin, the stratum corneum (SC), represents the main skin barrier. The SC lipids have a very exceptional composition, as the main lipid classes are ceramides (CER), long-chain fatty acids and cholesterol. Information on the function of each CER subclass and on the relation between CER lipid organisation and composition is of great importance to unravel the mechanism controlling the skin barrier function. Raman spectroscopy has been increasingly used for the study of intra- and inter-molecular structures of long-chain lipid compounds. In this study, we employed Raman spectroscopy to evaluate the effect of (1) the chain length and (2) the polar head architecture on the conformational order and organisational behaviour of CERs. The relation between the structure and the stability of the organisation was studied by monitoring the thermotropic response of each CER in the temperature range between 25 and 95 °C. This work enabled the determination of a correlation between the gauche/trans ratio in the νCC region and the state of the lateral packing. Moreover, it was shown that -OH groups in the α position of the fatty acids reduce the stability while long alkyl chains reinforces the intra- and inter-chains order.

Authors+Show Affiliations

EA 4041 Group Chimie Analytique de Paris Sud, Faculty of Pharmacy, Paris-Sud University, 92290 Châtenay-Malabry, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21069298

Citation

Guillard, Emmanuelle, et al. "Thermal Dependence of Raman Descriptors of Ceramides. Part II: Effect of Chains Lengths and Head Group Structures." Analytical and Bioanalytical Chemistry, vol. 399, no. 3, 2011, pp. 1201-13.
Guillard E, Tfayli A, Manfait M, et al. Thermal dependence of Raman descriptors of ceramides. Part II: effect of chains lengths and head group structures. Anal Bioanal Chem. 2011;399(3):1201-13.
Guillard, E., Tfayli, A., Manfait, M., & Baillet-Guffroy, A. (2011). Thermal dependence of Raman descriptors of ceramides. Part II: effect of chains lengths and head group structures. Analytical and Bioanalytical Chemistry, 399(3), 1201-13. https://doi.org/10.1007/s00216-010-4389-x
Guillard E, et al. Thermal Dependence of Raman Descriptors of Ceramides. Part II: Effect of Chains Lengths and Head Group Structures. Anal Bioanal Chem. 2011;399(3):1201-13. PubMed PMID: 21069298.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Thermal dependence of Raman descriptors of ceramides. Part II: effect of chains lengths and head group structures. AU - Guillard,Emmanuelle, AU - Tfayli,Ali, AU - Manfait,Michel, AU - Baillet-Guffroy,Arlette, Y1 - 2010/11/11/ PY - 2010/08/11/received PY - 2010/10/25/accepted PY - 2010/10/17/revised PY - 2010/11/12/entrez PY - 2010/11/12/pubmed PY - 2011/5/24/medline SP - 1201 EP - 13 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 399 IS - 3 N2 - The outermost layer of the mammalian skin, the stratum corneum (SC), represents the main skin barrier. The SC lipids have a very exceptional composition, as the main lipid classes are ceramides (CER), long-chain fatty acids and cholesterol. Information on the function of each CER subclass and on the relation between CER lipid organisation and composition is of great importance to unravel the mechanism controlling the skin barrier function. Raman spectroscopy has been increasingly used for the study of intra- and inter-molecular structures of long-chain lipid compounds. In this study, we employed Raman spectroscopy to evaluate the effect of (1) the chain length and (2) the polar head architecture on the conformational order and organisational behaviour of CERs. The relation between the structure and the stability of the organisation was studied by monitoring the thermotropic response of each CER in the temperature range between 25 and 95 °C. This work enabled the determination of a correlation between the gauche/trans ratio in the νCC region and the state of the lateral packing. Moreover, it was shown that -OH groups in the α position of the fatty acids reduce the stability while long alkyl chains reinforces the intra- and inter-chains order. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/21069298/Thermal_dependence_of_Raman_descriptors_of_ceramides__Part_II:_effect_of_chains_lengths_and_head_group_structures_ L2 - https://dx.doi.org/10.1007/s00216-010-4389-x DB - PRIME DP - Unbound Medicine ER -