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Application of recombinant SMR-domain containing protein of angiostrongylus cantonensis in immunoblot diagnosis of human angiostrongyliasis.
Southeast Asian J Trop Med Public Health. 2010 Jul; 41(4):785-99.SA

Abstract

The aim of this study was to find novel proteins expressed from an Angiostrongylus cantonensis adult female worm cDNA library for serodiagnosis of angiostrongyliasis. An immuno-dominant clone, fAC22, was identified by immunoscreening with pooled positive sera from proven angiostrongyliasis patients. The clone contained an open reading frame of 2,136 bp encoding a 80.5 kDa protein with a predicted isoelectric point of 5.8. The deduced amino acid sequence (712 amino acids) contained the conserved domain of Small mutS related (Smr) superfamily protein, with similarity with the Smr domain protein of Brugia malayi. The fusion His-tagged 81 kDa recombinant protein expressed as inclusion body in Escherichia coli was solubilized and purified by Ni-affinity chromatography for use in immunoblot analysis. Its sensitivity, specificity, positive and negative predictive values in immunodiagnostic test was 93.5, 91.5, 79.0 and 97.5%, respectively. Although some cross-reactivity of the antigen was observed among gnathostomiasis, bancroftian filariasis, ascariasis, echinococcosis, paragonimiasis and opisthorchiasis, sera from 14 other infections were all negative. These data indicate its possible application in immunodiagnosis of clinically suspected angiostrongyliasis. Key words: Angiostrongylus cantonensis,eosinophilic meningitis, recombinant fusion protein, immunodiagnosis

Authors+Show Affiliations

Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21073053

Citation

Vitta, Apichat, et al. "Application of Recombinant SMR-domain Containing Protein of Angiostrongylus Cantonensis in Immunoblot Diagnosis of Human Angiostrongyliasis." The Southeast Asian Journal of Tropical Medicine and Public Health, vol. 41, no. 4, 2010, pp. 785-99.
Vitta A, Yoshino TP, Kalambaheti T, et al. Application of recombinant SMR-domain containing protein of angiostrongylus cantonensis in immunoblot diagnosis of human angiostrongyliasis. Southeast Asian J Trop Med Public Health. 2010;41(4):785-99.
Vitta, A., Yoshino, T. P., Kalambaheti, T., Komalamisra, C., Waikagul, J., Ruangsittichai, J., & Dekumyoy, P. (2010). Application of recombinant SMR-domain containing protein of angiostrongylus cantonensis in immunoblot diagnosis of human angiostrongyliasis. The Southeast Asian Journal of Tropical Medicine and Public Health, 41(4), 785-99.
Vitta A, et al. Application of Recombinant SMR-domain Containing Protein of Angiostrongylus Cantonensis in Immunoblot Diagnosis of Human Angiostrongyliasis. Southeast Asian J Trop Med Public Health. 2010;41(4):785-99. PubMed PMID: 21073053.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of recombinant SMR-domain containing protein of angiostrongylus cantonensis in immunoblot diagnosis of human angiostrongyliasis. AU - Vitta,Apichat, AU - Yoshino,Timothy P, AU - Kalambaheti,Thareerat, AU - Komalamisra,Chalit, AU - Waikagul,Jitra, AU - Ruangsittichai,Jiraporn, AU - Dekumyoy,Paron, PY - 2010/11/16/entrez PY - 2010/11/16/pubmed PY - 2010/12/14/medline SP - 785 EP - 99 JF - The Southeast Asian journal of tropical medicine and public health JO - Southeast Asian J Trop Med Public Health VL - 41 IS - 4 N2 - The aim of this study was to find novel proteins expressed from an Angiostrongylus cantonensis adult female worm cDNA library for serodiagnosis of angiostrongyliasis. An immuno-dominant clone, fAC22, was identified by immunoscreening with pooled positive sera from proven angiostrongyliasis patients. The clone contained an open reading frame of 2,136 bp encoding a 80.5 kDa protein with a predicted isoelectric point of 5.8. The deduced amino acid sequence (712 amino acids) contained the conserved domain of Small mutS related (Smr) superfamily protein, with similarity with the Smr domain protein of Brugia malayi. The fusion His-tagged 81 kDa recombinant protein expressed as inclusion body in Escherichia coli was solubilized and purified by Ni-affinity chromatography for use in immunoblot analysis. Its sensitivity, specificity, positive and negative predictive values in immunodiagnostic test was 93.5, 91.5, 79.0 and 97.5%, respectively. Although some cross-reactivity of the antigen was observed among gnathostomiasis, bancroftian filariasis, ascariasis, echinococcosis, paragonimiasis and opisthorchiasis, sera from 14 other infections were all negative. These data indicate its possible application in immunodiagnosis of clinically suspected angiostrongyliasis. Key words: Angiostrongylus cantonensis,eosinophilic meningitis, recombinant fusion protein, immunodiagnosis SN - 0125-1562 UR - https://www.unboundmedicine.com/medline/citation/21073053/Application_of_recombinant_SMR_domain_containing_protein_of_angiostrongylus_cantonensis_in_immunoblot_diagnosis_of_human_angiostrongyliasis_ L2 - http://www.diseaseinfosearch.org/result/468 DB - PRIME DP - Unbound Medicine ER -