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Antibacterial activity and cytotoxicity of two novel cross-linking antibacterial monomers on oral pathogens.

Abstract

OBJECTIVES

The antibacterial activity and cytotoxicity of two novel cross-linking antibacterial monomers, 2-methacryloxylethyl dodecyl methyl ammonium bromide (MAE-DB) and 2-methacryloxylethyl hexadecyl methyl ammonium bromide (MAE-HB) were tested in this study.

DESIGN

The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of unpolymerized MAE-DB and MAE-HB against eight strains of oral bacteria were tested using a broth dilution test. Time-kill determinations were performed to examine the kinetics of unpolymerized MAE-DB and MAE-HB against Streptococcus mutans UA159 and Streptococcus sanguinis ATCC6715. Bacterial morphology was observed using a field emission scanning electron microscope (Fe-SEM). The cytotoxicity of unpolymerized two new monomers and Bis-GMA on the human gingival fibroblast cell line H2620 was assessed using a methyl thiazolyl tetrazolium assay.

RESULTS

Unpolymerized MAE-DB and MAE-HB showed strong bactericidal activity against oral bacteria. The MBC value of MAE-DB ranged from 12.2 to 24.4μg/ml and the MBC value of MAE-HB ranged from 6.2 to 48.8μg/ml. Time-kill determinations indicated that unpolymerized MAE-DB and MAE-HB had rapid killing effects against S. mutans UA159 and S. sanguinis ATCC6715 at the concentration of 4× MBC. The Fe-SEM observation showed that MAE-DB and MAE-HB could disturb the integrity of bacteria and cause lysis of bacterial cells. The median lethal concentration values on human gingival fibroblast for both monomers were between 10 and 20μg/ml, and greater than that of Bis-GMA.

CONCLUSIONS

Unpolymerized MAE-DB and MAE-HB monomers had strong bactericidal activity against eight strains of oral bacteria. Their cytotoxicities were less than that of Bis-GMA.

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  • Authors+Show Affiliations

    ,

    Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Changle West Road 145, Xi'an 710032, PR China.

    , , , , ,

    Source

    Archives of oral biology 56:4 2011 Apr pg 367-73

    MeSH

    Anti-Bacterial Agents
    Bacteria
    Cells, Cultured
    Colony Count, Microbial
    Dental Caries
    Fibroblasts
    Gingiva
    Humans
    Methacrylates
    Microbial Sensitivity Tests
    Mouth
    Periodontal Diseases
    Polymers
    Quaternary Ammonium Compounds

    Pub Type(s)

    Comparative Study
    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    21074143

    Citation

    Huang, Li, et al. "Antibacterial Activity and Cytotoxicity of Two Novel Cross-linking Antibacterial Monomers On Oral Pathogens." Archives of Oral Biology, vol. 56, no. 4, 2011, pp. 367-73.
    Huang L, Xiao YH, Xing XD, et al. Antibacterial activity and cytotoxicity of two novel cross-linking antibacterial monomers on oral pathogens. Arch Oral Biol. 2011;56(4):367-73.
    Huang, L., Xiao, Y. H., Xing, X. D., Li, F., Ma, S., Qi, L. L., & Chen, J. H. (2011). Antibacterial activity and cytotoxicity of two novel cross-linking antibacterial monomers on oral pathogens. Archives of Oral Biology, 56(4), pp. 367-73. doi:10.1016/j.archoralbio.2010.10.011.
    Huang L, et al. Antibacterial Activity and Cytotoxicity of Two Novel Cross-linking Antibacterial Monomers On Oral Pathogens. Arch Oral Biol. 2011;56(4):367-73. PubMed PMID: 21074143.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Antibacterial activity and cytotoxicity of two novel cross-linking antibacterial monomers on oral pathogens. AU - Huang,Li, AU - Xiao,Yu-Hong, AU - Xing,Xiao-Dong, AU - Li,Fang, AU - Ma,Sai, AU - Qi,Ling-Ling, AU - Chen,Ji-Hua, Y1 - 2010/11/11/ PY - 2010/03/09/received PY - 2010/10/11/revised PY - 2010/10/17/accepted PY - 2010/11/16/entrez PY - 2010/11/16/pubmed PY - 2011/8/11/medline SP - 367 EP - 73 JF - Archives of oral biology JO - Arch. Oral Biol. VL - 56 IS - 4 N2 - OBJECTIVES: The antibacterial activity and cytotoxicity of two novel cross-linking antibacterial monomers, 2-methacryloxylethyl dodecyl methyl ammonium bromide (MAE-DB) and 2-methacryloxylethyl hexadecyl methyl ammonium bromide (MAE-HB) were tested in this study. DESIGN: The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of unpolymerized MAE-DB and MAE-HB against eight strains of oral bacteria were tested using a broth dilution test. Time-kill determinations were performed to examine the kinetics of unpolymerized MAE-DB and MAE-HB against Streptococcus mutans UA159 and Streptococcus sanguinis ATCC6715. Bacterial morphology was observed using a field emission scanning electron microscope (Fe-SEM). The cytotoxicity of unpolymerized two new monomers and Bis-GMA on the human gingival fibroblast cell line H2620 was assessed using a methyl thiazolyl tetrazolium assay. RESULTS: Unpolymerized MAE-DB and MAE-HB showed strong bactericidal activity against oral bacteria. The MBC value of MAE-DB ranged from 12.2 to 24.4μg/ml and the MBC value of MAE-HB ranged from 6.2 to 48.8μg/ml. Time-kill determinations indicated that unpolymerized MAE-DB and MAE-HB had rapid killing effects against S. mutans UA159 and S. sanguinis ATCC6715 at the concentration of 4× MBC. The Fe-SEM observation showed that MAE-DB and MAE-HB could disturb the integrity of bacteria and cause lysis of bacterial cells. The median lethal concentration values on human gingival fibroblast for both monomers were between 10 and 20μg/ml, and greater than that of Bis-GMA. CONCLUSIONS: Unpolymerized MAE-DB and MAE-HB monomers had strong bactericidal activity against eight strains of oral bacteria. Their cytotoxicities were less than that of Bis-GMA. SN - 1879-1506 UR - https://www.unboundmedicine.com/medline/citation/21074143/Antibacterial_activity_and_cytotoxicity_of_two_novel_cross_linking_antibacterial_monomers_on_oral_pathogens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-9969(10)00311-0 DB - PRIME DP - Unbound Medicine ER -