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Fur negatively regulates hns and is required for the expression of HilA and virulence in Salmonella enterica serovar Typhimurium.
J Bacteriol. 2011 Jan; 193(2):497-505.JB

Abstract

Iron is an essential element for the survival of living cells. However, excess iron is toxic, and its uptake is exquisitely regulated by the ferric uptake regulator, Fur. In Salmonella, the Salmonella pathogenicity island 1 (SPI-1) encodes a type three secretion system, which is required for invasion of host epithelial cells in the small intestine. A major activator of SPI-1 is HilA, which is encoded within SPI-1. One known regulator of hilA is Fur. The mechanism of hilA regulation by Fur is unknown. We report here that Fur is required for virulence in Salmonella enterica serovar Typhimurium and that Fur is required for the activation of hilA, as well as of other HilA-dependent genes, invF and sipC. The Fur-dependent regulation of hilA was independent of PhoP, a known repressor of hilA. Instead, the expression of the gene coding for the histone-like protein, hns, was significantly derepressed in the fur mutant. Indeed, the activation of hilA by Fur was dependent on 28 nucleotides located upstream of hns. Moreover, we used chromatin immunoprecipitation to show that Fur bound, in vivo, to the upstream region of hns in a metal-dependent fashion. Finally, deletion of fur in an hns mutant resulted in Fur-independent activation of hilA. In conclusion, Fur activates hilA by repressing the expression of hns.

Authors+Show Affiliations

Department of Microbiology, North Carolina State University, Raleigh, NC 27695-7615, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21075923

Citation

Troxell, Bryan, et al. "Fur Negatively Regulates Hns and Is Required for the Expression of HilA and Virulence in Salmonella Enterica Serovar Typhimurium." Journal of Bacteriology, vol. 193, no. 2, 2011, pp. 497-505.
Troxell B, Sikes ML, Fink RC, et al. Fur negatively regulates hns and is required for the expression of HilA and virulence in Salmonella enterica serovar Typhimurium. J Bacteriol. 2011;193(2):497-505.
Troxell, B., Sikes, M. L., Fink, R. C., Vazquez-Torres, A., Jones-Carson, J., & Hassan, H. M. (2011). Fur negatively regulates hns and is required for the expression of HilA and virulence in Salmonella enterica serovar Typhimurium. Journal of Bacteriology, 193(2), 497-505. https://doi.org/10.1128/JB.00942-10
Troxell B, et al. Fur Negatively Regulates Hns and Is Required for the Expression of HilA and Virulence in Salmonella Enterica Serovar Typhimurium. J Bacteriol. 2011;193(2):497-505. PubMed PMID: 21075923.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fur negatively regulates hns and is required for the expression of HilA and virulence in Salmonella enterica serovar Typhimurium. AU - Troxell,Bryan, AU - Sikes,Michael L, AU - Fink,Ryan C, AU - Vazquez-Torres,Andres, AU - Jones-Carson,Jessica, AU - Hassan,Hosni M, Y1 - 2010/11/12/ PY - 2010/11/16/entrez PY - 2010/11/16/pubmed PY - 2011/1/29/medline SP - 497 EP - 505 JF - Journal of bacteriology JO - J Bacteriol VL - 193 IS - 2 N2 - Iron is an essential element for the survival of living cells. However, excess iron is toxic, and its uptake is exquisitely regulated by the ferric uptake regulator, Fur. In Salmonella, the Salmonella pathogenicity island 1 (SPI-1) encodes a type three secretion system, which is required for invasion of host epithelial cells in the small intestine. A major activator of SPI-1 is HilA, which is encoded within SPI-1. One known regulator of hilA is Fur. The mechanism of hilA regulation by Fur is unknown. We report here that Fur is required for virulence in Salmonella enterica serovar Typhimurium and that Fur is required for the activation of hilA, as well as of other HilA-dependent genes, invF and sipC. The Fur-dependent regulation of hilA was independent of PhoP, a known repressor of hilA. Instead, the expression of the gene coding for the histone-like protein, hns, was significantly derepressed in the fur mutant. Indeed, the activation of hilA by Fur was dependent on 28 nucleotides located upstream of hns. Moreover, we used chromatin immunoprecipitation to show that Fur bound, in vivo, to the upstream region of hns in a metal-dependent fashion. Finally, deletion of fur in an hns mutant resulted in Fur-independent activation of hilA. In conclusion, Fur activates hilA by repressing the expression of hns. SN - 1098-5530 UR - https://www.unboundmedicine.com/medline/citation/21075923/Fur_negatively_regulates_hns_and_is_required_for_the_expression_of_HilA_and_virulence_in_Salmonella_enterica_serovar_Typhimurium_ L2 - https://journals.asm.org/doi/10.1128/JB.00942-10?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -