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Bioanalytical applications of capillary electrophoresis with laser-induced native fluorescence detection.
Bioanalysis. 2010 Sep; 2(9):1641-53.B

Abstract

In this article we describe recent developments and applications of capillary electrophoresis (CE) coupled with laser-induced native fluorescence (LINF) detection in the analysis of biological, pharmaceutical and environmental samples. Compared with traditional UV absorbance detection used in CE, the LINF technique can greatly improve the concentration sensitivity of CE without the need for derivatization; the only requirement being that the analyte must have native fluorescence. Instrumentation and laser sources used in CE-LINF are summarized and specific applications of CE-LINF to small-biomolecule analysis, profiling of human biofluids, detection of native fluorescent peptides and proteins, single-cell analysis and the use of online sample preconcentration methods are also reviewed in detail.

Authors+Show Affiliations

Centre for Analytical Bioscience, School of Pharmacy, University of Nottingham, Nottingham, NG7 2RD, UK.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

21083292

Citation

Tseng, Hua-Ming, et al. "Bioanalytical Applications of Capillary Electrophoresis With Laser-induced Native Fluorescence Detection." Bioanalysis, vol. 2, no. 9, 2010, pp. 1641-53.
Tseng HM, Li Y, Barrett DA. Bioanalytical applications of capillary electrophoresis with laser-induced native fluorescence detection. Bioanalysis. 2010;2(9):1641-53.
Tseng, H. M., Li, Y., & Barrett, D. A. (2010). Bioanalytical applications of capillary electrophoresis with laser-induced native fluorescence detection. Bioanalysis, 2(9), 1641-53. https://doi.org/10.4155/bio.10.72
Tseng HM, Li Y, Barrett DA. Bioanalytical Applications of Capillary Electrophoresis With Laser-induced Native Fluorescence Detection. Bioanalysis. 2010;2(9):1641-53. PubMed PMID: 21083292.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bioanalytical applications of capillary electrophoresis with laser-induced native fluorescence detection. AU - Tseng,Hua-Ming, AU - Li,Yin, AU - Barrett,David A, PY - 2010/11/19/entrez PY - 2010/11/19/pubmed PY - 2011/3/15/medline SP - 1641 EP - 53 JF - Bioanalysis JO - Bioanalysis VL - 2 IS - 9 N2 - In this article we describe recent developments and applications of capillary electrophoresis (CE) coupled with laser-induced native fluorescence (LINF) detection in the analysis of biological, pharmaceutical and environmental samples. Compared with traditional UV absorbance detection used in CE, the LINF technique can greatly improve the concentration sensitivity of CE without the need for derivatization; the only requirement being that the analyte must have native fluorescence. Instrumentation and laser sources used in CE-LINF are summarized and specific applications of CE-LINF to small-biomolecule analysis, profiling of human biofluids, detection of native fluorescent peptides and proteins, single-cell analysis and the use of online sample preconcentration methods are also reviewed in detail. SN - 1757-6199 UR - https://www.unboundmedicine.com/medline/citation/21083292/Bioanalytical_applications_of_capillary_electrophoresis_with_laser_induced_native_fluorescence_detection_ DB - PRIME DP - Unbound Medicine ER -