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The performance of a component-based allergen microarray for the diagnosis of kiwifruit allergy.
Clin Exp Allergy 2011; 41(1):129-36CE

Abstract

BACKGROUND

Allergy to kiwifruit is increasingly reported across Europe. Currently, the reliability of its diagnosis by the measurement of allergen-specific IgE with extracts or by skin testing with fresh fruits is unsatisfying.

OBJECTIVE

To evaluate the usefulness of a component-based allergen microarray for the diagnosis of kiwifruit allergy in a large group of patients.

METHODS

With an allergen microarray, we measured specific IgE and IgG4 levels to a panel of nine kiwifruit allergens in sera of 237 individuals with kiwifruit allergy. Sera from 198 allergic patients without kiwifruit allergy served as controls. Furthermore, we determined the extent of sensitization to latex.

RESULTS

The panel of kiwifruit allergens showed a diagnostic sensitivity of 66%, a specificity of 56% and a positive predictive value of 73%. Sera from kiwifruit-allergic patients contained significantly more frequently Act d 1-specific IgE than sera from control patients. Furthermore, 51% of the positive sera contained IgE directed to a single allergen, namely Act d 1 (45%), Act d 9 (27%) or Act d 7 (13%). Within the control group, 36% sera recognized a single allergen. Out of those, 48% were positive to the cross-reactive glycoallergen Act d 7, 43% to the profilin Act d 9 and only 5% to Act d 1. Allergen-specific IgG4 levels did not differ between kiwifruit-allergic and -tolerant patients. Kiwifruit- and latex-allergic patients contained Hev b 11-specific IgE significantly more frequently than latex-allergic patients without kiwifruit allergy.

CONCLUSIONS

Act d 1 can be considered a marker allergen for genuine sensitization to kiwifruit. We demonstrated that a component-based kiwifruit allergen microarray would improve the prognostic value of in vitro diagnostic tests.

Authors+Show Affiliations

Department of Pathophysiology and Allergy Research, Medical University of Vienna, Vienna, Austria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21083775

Citation

Bublin, M, et al. "The Performance of a Component-based Allergen Microarray for the Diagnosis of Kiwifruit Allergy." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, vol. 41, no. 1, 2011, pp. 129-36.
Bublin M, Dennstedt S, Buchegger M, et al. The performance of a component-based allergen microarray for the diagnosis of kiwifruit allergy. Clin Exp Allergy. 2011;41(1):129-36.
Bublin, M., Dennstedt, S., Buchegger, M., Antonietta Ciardiello, M., Bernardi, M. L., Tuppo, L., ... Breiteneder, H. (2011). The performance of a component-based allergen microarray for the diagnosis of kiwifruit allergy. Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, 41(1), pp. 129-36. doi:10.1111/j.1365-2222.2010.03619.x.
Bublin M, et al. The Performance of a Component-based Allergen Microarray for the Diagnosis of Kiwifruit Allergy. Clin Exp Allergy. 2011;41(1):129-36. PubMed PMID: 21083775.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The performance of a component-based allergen microarray for the diagnosis of kiwifruit allergy. AU - Bublin,M, AU - Dennstedt,S, AU - Buchegger,M, AU - Antonietta Ciardiello,M, AU - Bernardi,M L, AU - Tuppo,L, AU - Harwanegg,C, AU - Hafner,C, AU - Ebner,C, AU - Ballmer-Weber,B K, AU - Knulst,A, AU - Hoffmann-Sommergruber,K, AU - Radauer,C, AU - Mari,A, AU - Breiteneder,H, Y1 - 2010/11/17/ PY - 2010/11/19/entrez PY - 2010/11/19/pubmed PY - 2011/10/14/medline SP - 129 EP - 36 JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology JO - Clin. Exp. Allergy VL - 41 IS - 1 N2 - BACKGROUND: Allergy to kiwifruit is increasingly reported across Europe. Currently, the reliability of its diagnosis by the measurement of allergen-specific IgE with extracts or by skin testing with fresh fruits is unsatisfying. OBJECTIVE: To evaluate the usefulness of a component-based allergen microarray for the diagnosis of kiwifruit allergy in a large group of patients. METHODS: With an allergen microarray, we measured specific IgE and IgG4 levels to a panel of nine kiwifruit allergens in sera of 237 individuals with kiwifruit allergy. Sera from 198 allergic patients without kiwifruit allergy served as controls. Furthermore, we determined the extent of sensitization to latex. RESULTS: The panel of kiwifruit allergens showed a diagnostic sensitivity of 66%, a specificity of 56% and a positive predictive value of 73%. Sera from kiwifruit-allergic patients contained significantly more frequently Act d 1-specific IgE than sera from control patients. Furthermore, 51% of the positive sera contained IgE directed to a single allergen, namely Act d 1 (45%), Act d 9 (27%) or Act d 7 (13%). Within the control group, 36% sera recognized a single allergen. Out of those, 48% were positive to the cross-reactive glycoallergen Act d 7, 43% to the profilin Act d 9 and only 5% to Act d 1. Allergen-specific IgG4 levels did not differ between kiwifruit-allergic and -tolerant patients. Kiwifruit- and latex-allergic patients contained Hev b 11-specific IgE significantly more frequently than latex-allergic patients without kiwifruit allergy. CONCLUSIONS: Act d 1 can be considered a marker allergen for genuine sensitization to kiwifruit. We demonstrated that a component-based kiwifruit allergen microarray would improve the prognostic value of in vitro diagnostic tests. SN - 1365-2222 UR - https://www.unboundmedicine.com/medline/citation/21083775/The_performance_of_a_component_based_allergen_microarray_for_the_diagnosis_of_kiwifruit_allergy_ L2 - https://doi.org/10.1111/j.1365-2222.2010.03619.x DB - PRIME DP - Unbound Medicine ER -