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Determination of uric acid in human urine by capillary zone electrophoresis with indirect laser-induced fluorescence detection.
J Sep Sci. 2010 Dec; 33(23-24):3710-6.JS

Abstract

A CZE with indirect LIF detection method was used for the determination of uric acid (UA) in human urine. UA and its coexisting analytes (i.e. hypoxanthine, xanthine and ascorbic acid) could be well separated within 4.5 min at a voltage of 25 kV with 25°C cartridge temperature in a running buffer composed of 5 mM sodium borate, 10% methanol (v/v) and 0.3 μM fluorescein sodium (apparent pH of the final mixed hydro-organic solution of sodium borate, methanol and fluorescein is 9.5). Under the optimum condition, the method has good linearity relationships (correlation coefficients: 0.9973-0.9987) with ranges of 25-500, 25-350, 25-250 and 25-300 μg/mL for hypoxanthine, ascorbic acid, xanthine and UA, respectively. The detection limits for the analytes were in the range of 0.29-0.90 μg/mL. The intra-day RSD values for migration times and peak areas were less than 0.43 and 3.27%, respectively. This method was applied to the quantitation of UA in human urine with recoveries in the range of 93.1-107.3%.

Authors+Show Affiliations

College of Chemical Engineering, Sichuan University, Chengdu, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21089057

Citation

Yang, Jiajia, et al. "Determination of Uric Acid in Human Urine By Capillary Zone Electrophoresis With Indirect Laser-induced Fluorescence Detection." Journal of Separation Science, vol. 33, no. 23-24, 2010, pp. 3710-6.
Yang J, Hu M, Cai Y, et al. Determination of uric acid in human urine by capillary zone electrophoresis with indirect laser-induced fluorescence detection. J Sep Sci. 2010;33(23-24):3710-6.
Yang, J., Hu, M., Cai, Y., Tang, J., & Li, H. (2010). Determination of uric acid in human urine by capillary zone electrophoresis with indirect laser-induced fluorescence detection. Journal of Separation Science, 33(23-24), 3710-6. https://doi.org/10.1002/jssc.201000334
Yang J, et al. Determination of Uric Acid in Human Urine By Capillary Zone Electrophoresis With Indirect Laser-induced Fluorescence Detection. J Sep Sci. 2010;33(23-24):3710-6. PubMed PMID: 21089057.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of uric acid in human urine by capillary zone electrophoresis with indirect laser-induced fluorescence detection. AU - Yang,Jiajia, AU - Hu,Menglin, AU - Cai,Yuanli, AU - Tang,Jing, AU - Li,Hui, PY - 2010/11/20/entrez PY - 2010/11/23/pubmed PY - 2011/3/24/medline SP - 3710 EP - 6 JF - Journal of separation science JO - J Sep Sci VL - 33 IS - 23-24 N2 - A CZE with indirect LIF detection method was used for the determination of uric acid (UA) in human urine. UA and its coexisting analytes (i.e. hypoxanthine, xanthine and ascorbic acid) could be well separated within 4.5 min at a voltage of 25 kV with 25°C cartridge temperature in a running buffer composed of 5 mM sodium borate, 10% methanol (v/v) and 0.3 μM fluorescein sodium (apparent pH of the final mixed hydro-organic solution of sodium borate, methanol and fluorescein is 9.5). Under the optimum condition, the method has good linearity relationships (correlation coefficients: 0.9973-0.9987) with ranges of 25-500, 25-350, 25-250 and 25-300 μg/mL for hypoxanthine, ascorbic acid, xanthine and UA, respectively. The detection limits for the analytes were in the range of 0.29-0.90 μg/mL. The intra-day RSD values for migration times and peak areas were less than 0.43 and 3.27%, respectively. This method was applied to the quantitation of UA in human urine with recoveries in the range of 93.1-107.3%. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/21089057/Determination_of_uric_acid_in_human_urine_by_capillary_zone_electrophoresis_with_indirect_laser_induced_fluorescence_detection_ L2 - https://doi.org/10.1002/jssc.201000334 DB - PRIME DP - Unbound Medicine ER -