Tags

Type your tag names separated by a space and hit enter

Interactions of saturated, n-6 and n-3 polyunsaturated fatty acids to modulate arachidonic acid metabolism.
J Lipid Res. 1990 Feb; 31(2):271-7.JL

Abstract

Anti-thrombotic effects of omega-3 (n-3) fatty acids are believed to be due to their ability to reduce arachidonic acid levels. Therefore, weanling rats were fed n-3 acids in the form of linseed oil (18:3n-3) or fish oil (containing 20:5n-3 and 22:6n-3) in diets containing high levels of either saturated fatty acids (hydrogenated beef tallow) or high levels of linoleic acid (safflower oil) for 4 weeks. The effect of diet on the rate-limiting enzyme of arachidonic acid biosynthesis (delta 6-desaturase) and on the lipid composition of hepatic microsomal membrane was determined. Both linseed oil- or fish oil-containing diets inhibited conversion of linoleic acid to gamma-linolenic acid. Inhibition was greater with fish oil than with linseed oil, only when fed with saturated fat. delta 6-Desaturase activity was not affected when n-3 fatty acids were fed with high levels of n-6 fatty acids. Arachidonic acid content of serum lipids and hepatic microsomal phospholipids was lower when n-3 fatty acids were fed in combination with beef tallow but not when fed with safflower oil. Similarly, n-3 fatty acids (18:3n-3, 20:5n-3, 22:5n-3, and 22:6n-3) accumulated to a greater extent when n-3 fatty acids were fed with beef tallow than with safflower oil. These observations indicate that the efficacy of n-3 fatty acids in reducing arachidonic acid level is dependent on the linoleic acid to saturated fatty acid ratio of the diet consumed.

Authors+Show Affiliations

Nutrition and Metabolism Research Group, University of Alberta, Edmonton, Canada.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2109031

Citation

Garg, M L., et al. "Interactions of Saturated, N-6 and N-3 Polyunsaturated Fatty Acids to Modulate Arachidonic Acid Metabolism." Journal of Lipid Research, vol. 31, no. 2, 1990, pp. 271-7.
Garg ML, Thomson AB, Clandinin MT. Interactions of saturated, n-6 and n-3 polyunsaturated fatty acids to modulate arachidonic acid metabolism. J Lipid Res. 1990;31(2):271-7.
Garg, M. L., Thomson, A. B., & Clandinin, M. T. (1990). Interactions of saturated, n-6 and n-3 polyunsaturated fatty acids to modulate arachidonic acid metabolism. Journal of Lipid Research, 31(2), 271-7.
Garg ML, Thomson AB, Clandinin MT. Interactions of Saturated, N-6 and N-3 Polyunsaturated Fatty Acids to Modulate Arachidonic Acid Metabolism. J Lipid Res. 1990;31(2):271-7. PubMed PMID: 2109031.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interactions of saturated, n-6 and n-3 polyunsaturated fatty acids to modulate arachidonic acid metabolism. AU - Garg,M L, AU - Thomson,A B, AU - Clandinin,M T, PY - 1990/2/1/pubmed PY - 1990/2/1/medline PY - 1990/2/1/entrez SP - 271 EP - 7 JF - Journal of lipid research JO - J. Lipid Res. VL - 31 IS - 2 N2 - Anti-thrombotic effects of omega-3 (n-3) fatty acids are believed to be due to their ability to reduce arachidonic acid levels. Therefore, weanling rats were fed n-3 acids in the form of linseed oil (18:3n-3) or fish oil (containing 20:5n-3 and 22:6n-3) in diets containing high levels of either saturated fatty acids (hydrogenated beef tallow) or high levels of linoleic acid (safflower oil) for 4 weeks. The effect of diet on the rate-limiting enzyme of arachidonic acid biosynthesis (delta 6-desaturase) and on the lipid composition of hepatic microsomal membrane was determined. Both linseed oil- or fish oil-containing diets inhibited conversion of linoleic acid to gamma-linolenic acid. Inhibition was greater with fish oil than with linseed oil, only when fed with saturated fat. delta 6-Desaturase activity was not affected when n-3 fatty acids were fed with high levels of n-6 fatty acids. Arachidonic acid content of serum lipids and hepatic microsomal phospholipids was lower when n-3 fatty acids were fed in combination with beef tallow but not when fed with safflower oil. Similarly, n-3 fatty acids (18:3n-3, 20:5n-3, 22:5n-3, and 22:6n-3) accumulated to a greater extent when n-3 fatty acids were fed with beef tallow than with safflower oil. These observations indicate that the efficacy of n-3 fatty acids in reducing arachidonic acid level is dependent on the linoleic acid to saturated fatty acid ratio of the diet consumed. SN - 0022-2275 UR - https://www.unboundmedicine.com/medline/citation/2109031/Interactions_of_saturated_n_6_and_n_3_polyunsaturated_fatty_acids_to_modulate_arachidonic_acid_metabolism_ L2 - http://www.jlr.org/cgi/pmidlookup?view=long&pmid=2109031 DB - PRIME DP - Unbound Medicine ER -