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Quercetin ameliorates experimental autoimmune myocarditis in rats.
J Pharm Pharm Sci. 2010; 13(3):311-9.JP

Abstract

PURPOSE

Experimental autoimmune myocarditis (EAM) in rats is an animal model of human giant cell myocarditis and post-myocarditis dilated cardiomyopathy. The pathogenesis of EAM has not been elucidated, but there is accumulating evidence that cytokines secreted from monocytes/macrophages and T cells play a crucial role in the induction and progression of disease. Flavonoids are a large group of polyphenolic compounds abundantly present in the human diet, which scavenge oxygen radicals and have anti-inflammatory activities. Having in mind in vivo beneficial effects of flavonoid quercetin in different animal models of immunoinflammatory diseases such as experimental autoimmune encephalomyelitis and adjuvant arthritis, on the one side, and its in vitro suppressive effect on production of tumor necrosis factor-alpha (TNF-α) on the other side, we investigated the effects of quercetin on EAM in rats.

METHODS

Myocarditis was induced in Dark Agouti (DA) rats by injection of porcine cardiac myosin and quercetin at doses of 10 or 20 mg/kg was orally administered from days 0 to 21 after induction of disease. The severity of myocarditis was evaluated by determination of heart weight/body weight ratio (Hw/Bw) and histopathological examination of hearts. The levels of cytokines (TNF-α, IL-12, IL-17 and IL-10) in serum and lymph node cells (LNC) culture supernatants were measured by ELISA.

RESULTS

The rats treated with 20 mg/kg of quercetin had significantly decreased incidence of EAM, Hw/Bw, macroscopic and microscopic scores of hearts. Further, in EAM rats treated with quercetin levels of TNF-α and IL-17 were significantly lower, while the level of IL-10 was significantly higher both in serum and culture supernatants of LNC stimulated with concanavalin A compared with vehicle-treated animals.

CONCLUSIONS

The present study suggests that quercetin ameliorates EAM, at least in part, by interfering production of proinflammatory (TNF-α and IL-17) and/or anti-inflammatory (IL-10) cytokines.

Authors+Show Affiliations

Department of Microbiology and Immunology, Faculty of Pharmacy, Vojvode Stepe 450, 11221 Belgrade, Serbia. marinama@pharmacy.bg.ac.rsNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21092705

Citation

Milenković, Marina, et al. "Quercetin Ameliorates Experimental Autoimmune Myocarditis in Rats." Journal of Pharmacy & Pharmaceutical Sciences : a Publication of the Canadian Society for Pharmaceutical Sciences, Societe Canadienne Des Sciences Pharmaceutiques, vol. 13, no. 3, 2010, pp. 311-9.
Milenković M, Arsenović-Ranin N, Stojić-Vukanić Z, et al. Quercetin ameliorates experimental autoimmune myocarditis in rats. J Pharm Pharm Sci. 2010;13(3):311-9.
Milenković, M., Arsenović-Ranin, N., Stojić-Vukanić, Z., Bufan, B., Vučićević, D., & Jančić, I. (2010). Quercetin ameliorates experimental autoimmune myocarditis in rats. Journal of Pharmacy & Pharmaceutical Sciences : a Publication of the Canadian Society for Pharmaceutical Sciences, Societe Canadienne Des Sciences Pharmaceutiques, 13(3), 311-9.
Milenković M, et al. Quercetin Ameliorates Experimental Autoimmune Myocarditis in Rats. J Pharm Pharm Sci. 2010;13(3):311-9. PubMed PMID: 21092705.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quercetin ameliorates experimental autoimmune myocarditis in rats. AU - Milenković,Marina, AU - Arsenović-Ranin,Nevena, AU - Stojić-Vukanić,Zorica, AU - Bufan,Biljana, AU - Vučićević,Dragana, AU - Jančić,Ivan, PY - 2010/11/25/entrez PY - 2010/11/26/pubmed PY - 2011/6/7/medline SP - 311 EP - 9 JF - Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques JO - J Pharm Pharm Sci VL - 13 IS - 3 N2 - PURPOSE: Experimental autoimmune myocarditis (EAM) in rats is an animal model of human giant cell myocarditis and post-myocarditis dilated cardiomyopathy. The pathogenesis of EAM has not been elucidated, but there is accumulating evidence that cytokines secreted from monocytes/macrophages and T cells play a crucial role in the induction and progression of disease. Flavonoids are a large group of polyphenolic compounds abundantly present in the human diet, which scavenge oxygen radicals and have anti-inflammatory activities. Having in mind in vivo beneficial effects of flavonoid quercetin in different animal models of immunoinflammatory diseases such as experimental autoimmune encephalomyelitis and adjuvant arthritis, on the one side, and its in vitro suppressive effect on production of tumor necrosis factor-alpha (TNF-α) on the other side, we investigated the effects of quercetin on EAM in rats. METHODS: Myocarditis was induced in Dark Agouti (DA) rats by injection of porcine cardiac myosin and quercetin at doses of 10 or 20 mg/kg was orally administered from days 0 to 21 after induction of disease. The severity of myocarditis was evaluated by determination of heart weight/body weight ratio (Hw/Bw) and histopathological examination of hearts. The levels of cytokines (TNF-α, IL-12, IL-17 and IL-10) in serum and lymph node cells (LNC) culture supernatants were measured by ELISA. RESULTS: The rats treated with 20 mg/kg of quercetin had significantly decreased incidence of EAM, Hw/Bw, macroscopic and microscopic scores of hearts. Further, in EAM rats treated with quercetin levels of TNF-α and IL-17 were significantly lower, while the level of IL-10 was significantly higher both in serum and culture supernatants of LNC stimulated with concanavalin A compared with vehicle-treated animals. CONCLUSIONS: The present study suggests that quercetin ameliorates EAM, at least in part, by interfering production of proinflammatory (TNF-α and IL-17) and/or anti-inflammatory (IL-10) cytokines. SN - 1482-1826 UR - https://www.unboundmedicine.com/medline/citation/21092705/Quercetin_ameliorates_experimental_autoimmune_myocarditis_in_rats_ L2 - http://ejournals.library.ualberta.ca/index.php/JPPS/article/viewFile/7244/7310 DB - PRIME DP - Unbound Medicine ER -