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Morin improves urate excretion and kidney function through regulation of renal organic ion transporters in hyperuricemic mice.
J Pharm Pharm Sci. 2010; 13(3):411-27.JP

Abstract

PURPOSE

Morin (2′,3,4′,5,7-pentahydroxyflavone), a plant-derived flavonoid, has beneficial effects on hyperuricemia and renal dysfunction in animals. Since the decreased renal excretion of uric acid is the hallmark of hyperuricemia, here we studied the effects of oral morin administration on renal organic ion transporters in potassium oxonate-induced hyperuricemic mice.

METHODS

Hyperuricemia in mice was induced by potassium oxonate. Uric acid and creatinine concentrations in urine and serum, and fractional excretion of uric acid (FEUA) were performed to evaluate renal urate handling. Changes in expression levels of renal organic ion transporters were detected by Western blotting and semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) methods.

RESULTS

Morin treatment significantly increased urinary uric acid/creatinine ratio and FEUA, resulting in reduction of serum uric acid levels in hyperuricemic mice. And kidney conditions were also improved after morin treatment in this model. Protein and mRNA levels of glucose transporter 9 (mGLUT9) and urate transporter 1 (mURAT1) were significantly decreased, and of organic anion transporter 1 (mOAT1) were remarkably increased in the kidney of morin-treated hyperuricemic mice. Morin treatment also blocked down-regulations of renal organic cation and carnitine transporters (mOCT1, mOCT2, mOCTN1 and mOCTN2) in hyperuricemic mice.

CONCLUSION

These results suggest that morin exhibits the uricosuric effects via suppressing urate reabsorption and promoting urate secretion in the kidney of hyperuricemic mice and may help to attenuate deleterious effects of hyperuricemia with renal dysfunction.

Authors+Show Affiliations

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, P. R. China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21092713

Citation

Wang, Cai-Ping, et al. "Morin Improves Urate Excretion and Kidney Function Through Regulation of Renal Organic Ion Transporters in Hyperuricemic Mice." Journal of Pharmacy & Pharmaceutical Sciences : a Publication of the Canadian Society for Pharmaceutical Sciences, Societe Canadienne Des Sciences Pharmaceutiques, vol. 13, no. 3, 2010, pp. 411-27.
Wang CP, Wang X, Zhang X, et al. Morin improves urate excretion and kidney function through regulation of renal organic ion transporters in hyperuricemic mice. J Pharm Pharm Sci. 2010;13(3):411-27.
Wang, C. P., Wang, X., Zhang, X., Shi, Y. W., Liu, L., & Kong, L. D. (2010). Morin improves urate excretion and kidney function through regulation of renal organic ion transporters in hyperuricemic mice. Journal of Pharmacy & Pharmaceutical Sciences : a Publication of the Canadian Society for Pharmaceutical Sciences, Societe Canadienne Des Sciences Pharmaceutiques, 13(3), 411-27.
Wang CP, et al. Morin Improves Urate Excretion and Kidney Function Through Regulation of Renal Organic Ion Transporters in Hyperuricemic Mice. J Pharm Pharm Sci. 2010;13(3):411-27. PubMed PMID: 21092713.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Morin improves urate excretion and kidney function through regulation of renal organic ion transporters in hyperuricemic mice. AU - Wang,Cai-Ping, AU - Wang,Xing, AU - Zhang,Xian, AU - Shi,Yun-Wei, AU - Liu,Lei, AU - Kong,Ling-Dong, PY - 2010/11/25/entrez PY - 2010/11/26/pubmed PY - 2011/6/7/medline SP - 411 EP - 27 JF - Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques JO - J Pharm Pharm Sci VL - 13 IS - 3 N2 - PURPOSE: Morin (2′,3,4′,5,7-pentahydroxyflavone), a plant-derived flavonoid, has beneficial effects on hyperuricemia and renal dysfunction in animals. Since the decreased renal excretion of uric acid is the hallmark of hyperuricemia, here we studied the effects of oral morin administration on renal organic ion transporters in potassium oxonate-induced hyperuricemic mice. METHODS: Hyperuricemia in mice was induced by potassium oxonate. Uric acid and creatinine concentrations in urine and serum, and fractional excretion of uric acid (FEUA) were performed to evaluate renal urate handling. Changes in expression levels of renal organic ion transporters were detected by Western blotting and semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) methods. RESULTS: Morin treatment significantly increased urinary uric acid/creatinine ratio and FEUA, resulting in reduction of serum uric acid levels in hyperuricemic mice. And kidney conditions were also improved after morin treatment in this model. Protein and mRNA levels of glucose transporter 9 (mGLUT9) and urate transporter 1 (mURAT1) were significantly decreased, and of organic anion transporter 1 (mOAT1) were remarkably increased in the kidney of morin-treated hyperuricemic mice. Morin treatment also blocked down-regulations of renal organic cation and carnitine transporters (mOCT1, mOCT2, mOCTN1 and mOCTN2) in hyperuricemic mice. CONCLUSION: These results suggest that morin exhibits the uricosuric effects via suppressing urate reabsorption and promoting urate secretion in the kidney of hyperuricemic mice and may help to attenuate deleterious effects of hyperuricemia with renal dysfunction. SN - 1482-1826 UR - https://www.unboundmedicine.com/medline/citation/21092713/Morin_improves_urate_excretion_and_kidney_function_through_regulation_of_renal_organic_ion_transporters_in_hyperuricemic_mice_ L2 - http://ejournals.library.ualberta.ca/index.php/JPPS/article/viewFile/7925/7377 DB - PRIME DP - Unbound Medicine ER -