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Prevalence of intestinal parasites and genotyping of Giardia intestinalis in pet shop puppies in east Japan.
Vet Parasitol. 2011 Feb 28; 176(1):74-8.VP

Abstract

The current study examined the prevalence of intestinal parasites and genotypes of Giardia intestinalis in puppies from nine pet shops in east Japan. Fresh fecal samples from 1794 puppies (≦3 months old) were collected on one occasion. Giardia spp. was examined for specific coproantigen using ELISA kit (SNAP®Giardia, IDEXX Laboratories, Inc., USA). Other intestinal parasites were detected microscopically using the formalin-ethyl acetate sedimentation technique. Genotyping was determined for the random 29 stool samples identified as Giardia spp. positive using PCR and direct sequencing of the glutamate dehydrogenase (gdh) gene. Overall prevalence of protozoan Giardia spp. and Cystoisospora spp. revealed 23.4% and 11.3%, respectively. Prevalence of ascarids, Strongyloides spp. and hookworms were recorded 1.8%, 1.1% and 0.1%, respectively. Protozoan Giardia spp. and Cystoisospora spp., thus, represent important pathogens among pet shop puppies. All genotyped G. intestinalis isolates were belonged to assemblage C or D, identified as dog-specific genotypes. Zoonotic assemblage A and B were not demonstrated. The result suggests that the risk of zoonotic transmission of G. intestinalis from pet shops puppies to humans may be quite low in Japan.

Authors+Show Affiliations

First Department of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University, Towada, Aomori 034-8628, Japan. naoitoh@vmas.kitasato-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21093154

Citation

Itoh, N, et al. "Prevalence of Intestinal Parasites and Genotyping of Giardia Intestinalis in Pet Shop Puppies in East Japan." Veterinary Parasitology, vol. 176, no. 1, 2011, pp. 74-8.
Itoh N, Itagaki T, Kawabata T, et al. Prevalence of intestinal parasites and genotyping of Giardia intestinalis in pet shop puppies in east Japan. Vet Parasitol. 2011;176(1):74-8.
Itoh, N., Itagaki, T., Kawabata, T., Konaka, T., Muraoka, N., Saeki, H., Kanai, K., Chikazawa, S., Hori, Y., Hoshi, F., & Higuchi, S. (2011). Prevalence of intestinal parasites and genotyping of Giardia intestinalis in pet shop puppies in east Japan. Veterinary Parasitology, 176(1), 74-8. https://doi.org/10.1016/j.vetpar.2010.10.048
Itoh N, et al. Prevalence of Intestinal Parasites and Genotyping of Giardia Intestinalis in Pet Shop Puppies in East Japan. Vet Parasitol. 2011 Feb 28;176(1):74-8. PubMed PMID: 21093154.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence of intestinal parasites and genotyping of Giardia intestinalis in pet shop puppies in east Japan. AU - Itoh,N, AU - Itagaki,T, AU - Kawabata,T, AU - Konaka,T, AU - Muraoka,N, AU - Saeki,H, AU - Kanai,K, AU - Chikazawa,S, AU - Hori,Y, AU - Hoshi,F, AU - Higuchi,S, Y1 - 2010/10/30/ PY - 2010/08/05/received PY - 2010/10/20/revised PY - 2010/10/20/accepted PY - 2010/11/25/entrez PY - 2010/11/26/pubmed PY - 2011/6/15/medline SP - 74 EP - 8 JF - Veterinary parasitology JO - Vet. Parasitol. VL - 176 IS - 1 N2 - The current study examined the prevalence of intestinal parasites and genotypes of Giardia intestinalis in puppies from nine pet shops in east Japan. Fresh fecal samples from 1794 puppies (≦3 months old) were collected on one occasion. Giardia spp. was examined for specific coproantigen using ELISA kit (SNAP®Giardia, IDEXX Laboratories, Inc., USA). Other intestinal parasites were detected microscopically using the formalin-ethyl acetate sedimentation technique. Genotyping was determined for the random 29 stool samples identified as Giardia spp. positive using PCR and direct sequencing of the glutamate dehydrogenase (gdh) gene. Overall prevalence of protozoan Giardia spp. and Cystoisospora spp. revealed 23.4% and 11.3%, respectively. Prevalence of ascarids, Strongyloides spp. and hookworms were recorded 1.8%, 1.1% and 0.1%, respectively. Protozoan Giardia spp. and Cystoisospora spp., thus, represent important pathogens among pet shop puppies. All genotyped G. intestinalis isolates were belonged to assemblage C or D, identified as dog-specific genotypes. Zoonotic assemblage A and B were not demonstrated. The result suggests that the risk of zoonotic transmission of G. intestinalis from pet shops puppies to humans may be quite low in Japan. SN - 1873-2550 UR - https://www.unboundmedicine.com/medline/citation/21093154/Prevalence_of_intestinal_parasites_and_genotyping_of_Giardia_intestinalis_in_pet_shop_puppies_in_east_Japan_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-4017(10)00613-8 DB - PRIME DP - Unbound Medicine ER -