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Cellular stress induces cytoplasmic RNA granules in fission yeast.
RNA. 2011 Jan; 17(1):120-33.RNA

Abstract

Severe stress causes plant and animal cells to form large cytoplasmic granules containing RNA and proteins. Here, we demonstrate the existence of stress-induced cytoplasmic RNA granules in Schizosaccharomyces pombe. Homologs to several known protein components of mammalian processing bodies and stress granules are found in fission yeast RNA granules. In contrast to mammalian cells, poly(A)-binding protein (Pabp) colocalizes in stress-induced granules with decapping protein. After glucose deprivation, protein kinase A (PKA) is required for accumulation of Pabp-positive granules and translational down-regulation. This is the first demonstration of a role for PKA in RNA granule formation. In mammals, the translation initiation protein eIF2α is a key regulator of formation of granules containing poly(A)-binding protein. In S. pombe, nonphosphorylatable eIF2α does not block but delays granule formation and subsequent clearance after exposure to hyperosmosis. At least two separate pathways in S. pombe appear to regulate stress-induced granules: pka1 mutants are fully proficient to form granules after hyperosmotic shock; conversely, eIF2α does not affect granule formation in glucose starvation. Further, we demonstrate a Pka1-dependent link between calcium perturbation and RNA granules, which has not been described earlier in any organism.

Authors+Show Affiliations

Department of Cell and Molecular Biology, Lundberg Laboratory, University of Gothenburg, S-405 30 Göteborg, Sweden.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21098141

Citation

Nilsson, Daniel, and Per Sunnerhagen. "Cellular Stress Induces Cytoplasmic RNA Granules in Fission Yeast." RNA (New York, N.Y.), vol. 17, no. 1, 2011, pp. 120-33.
Nilsson D, Sunnerhagen P. Cellular stress induces cytoplasmic RNA granules in fission yeast. RNA. 2011;17(1):120-33.
Nilsson, D., & Sunnerhagen, P. (2011). Cellular stress induces cytoplasmic RNA granules in fission yeast. RNA (New York, N.Y.), 17(1), 120-33. https://doi.org/10.1261/rna.2268111
Nilsson D, Sunnerhagen P. Cellular Stress Induces Cytoplasmic RNA Granules in Fission Yeast. RNA. 2011;17(1):120-33. PubMed PMID: 21098141.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cellular stress induces cytoplasmic RNA granules in fission yeast. AU - Nilsson,Daniel, AU - Sunnerhagen,Per, Y1 - 2010/11/22/ PY - 2010/11/25/entrez PY - 2010/11/26/pubmed PY - 2011/2/1/medline SP - 120 EP - 33 JF - RNA (New York, N.Y.) JO - RNA VL - 17 IS - 1 N2 - Severe stress causes plant and animal cells to form large cytoplasmic granules containing RNA and proteins. Here, we demonstrate the existence of stress-induced cytoplasmic RNA granules in Schizosaccharomyces pombe. Homologs to several known protein components of mammalian processing bodies and stress granules are found in fission yeast RNA granules. In contrast to mammalian cells, poly(A)-binding protein (Pabp) colocalizes in stress-induced granules with decapping protein. After glucose deprivation, protein kinase A (PKA) is required for accumulation of Pabp-positive granules and translational down-regulation. This is the first demonstration of a role for PKA in RNA granule formation. In mammals, the translation initiation protein eIF2α is a key regulator of formation of granules containing poly(A)-binding protein. In S. pombe, nonphosphorylatable eIF2α does not block but delays granule formation and subsequent clearance after exposure to hyperosmosis. At least two separate pathways in S. pombe appear to regulate stress-induced granules: pka1 mutants are fully proficient to form granules after hyperosmotic shock; conversely, eIF2α does not affect granule formation in glucose starvation. Further, we demonstrate a Pka1-dependent link between calcium perturbation and RNA granules, which has not been described earlier in any organism. SN - 1469-9001 UR - https://www.unboundmedicine.com/medline/citation/21098141/Cellular_stress_induces_cytoplasmic_RNA_granules_in_fission_yeast_ L2 - http://www.rnajournal.org/cgi/pmidlookup?view=long&pmid=21098141 DB - PRIME DP - Unbound Medicine ER -