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A novel chimeric lysin shows superiority to mupirocin for skin decolonization of methicillin-resistant and -sensitive Staphylococcus aureus strains.
Antimicrob Agents Chemother. 2011 Feb; 55(2):738-44.AA

Abstract

Staphylococcus aureus is a major human pathogen responsible for a number of serious and sometimes fatal infections. One of its reservoirs on the human body is the skin, which is known to be a source of invasive infection. The potential for an engineered staphylococcus-specific phage lysin (ClyS) to be used for topical decolonization is presented. We formulated ClyS into an ointment and applied it to a mouse model of skin colonization/infection with S. aureus. Unlike the standard topical antibacterial agent mupirocin, ClyS eradicated a significantly greater number of methicillin-susceptible S. aureus (MSSA) and -resistant S. aureus (MRSA) bacteria: a 3-log reduction with ClyS as opposed to a 2-log reduction with mupirocin in our model. The use of ClyS also demonstrated a decreased potential for the development of resistance by MRSA and MSSA organisms compared to that from the use of mupirocin in vitro. Because antibodies may affect enzyme function, we tested antibodies developed after repeated ClyS exposure for their effect on ClyS killing ability. Our results showed no inhibition of ClyS activity at various antibody titers. These data demonstrate the potential of developing ClyS as a novel class of topical antimicrobial agents specific to staphylococcus.

Authors+Show Affiliations

Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, New York, NY 10065, USA. mpastagia@rockefeller.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

21098252

Citation

Pastagia, Mina, et al. "A Novel Chimeric Lysin Shows Superiority to Mupirocin for Skin Decolonization of Methicillin-resistant and -sensitive Staphylococcus Aureus Strains." Antimicrobial Agents and Chemotherapy, vol. 55, no. 2, 2011, pp. 738-44.
Pastagia M, Euler C, Chahales P, et al. A novel chimeric lysin shows superiority to mupirocin for skin decolonization of methicillin-resistant and -sensitive Staphylococcus aureus strains. Antimicrob Agents Chemother. 2011;55(2):738-44.
Pastagia, M., Euler, C., Chahales, P., Fuentes-Duculan, J., Krueger, J. G., & Fischetti, V. A. (2011). A novel chimeric lysin shows superiority to mupirocin for skin decolonization of methicillin-resistant and -sensitive Staphylococcus aureus strains. Antimicrobial Agents and Chemotherapy, 55(2), 738-44. https://doi.org/10.1128/AAC.00890-10
Pastagia M, et al. A Novel Chimeric Lysin Shows Superiority to Mupirocin for Skin Decolonization of Methicillin-resistant and -sensitive Staphylococcus Aureus Strains. Antimicrob Agents Chemother. 2011;55(2):738-44. PubMed PMID: 21098252.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel chimeric lysin shows superiority to mupirocin for skin decolonization of methicillin-resistant and -sensitive Staphylococcus aureus strains. AU - Pastagia,Mina, AU - Euler,Chad, AU - Chahales,Peter, AU - Fuentes-Duculan,Judilyn, AU - Krueger,James G, AU - Fischetti,Vincent A, Y1 - 2010/11/22/ PY - 2010/11/25/entrez PY - 2010/11/26/pubmed PY - 2011/5/20/medline SP - 738 EP - 44 JF - Antimicrobial agents and chemotherapy JO - Antimicrob. Agents Chemother. VL - 55 IS - 2 N2 - Staphylococcus aureus is a major human pathogen responsible for a number of serious and sometimes fatal infections. One of its reservoirs on the human body is the skin, which is known to be a source of invasive infection. The potential for an engineered staphylococcus-specific phage lysin (ClyS) to be used for topical decolonization is presented. We formulated ClyS into an ointment and applied it to a mouse model of skin colonization/infection with S. aureus. Unlike the standard topical antibacterial agent mupirocin, ClyS eradicated a significantly greater number of methicillin-susceptible S. aureus (MSSA) and -resistant S. aureus (MRSA) bacteria: a 3-log reduction with ClyS as opposed to a 2-log reduction with mupirocin in our model. The use of ClyS also demonstrated a decreased potential for the development of resistance by MRSA and MSSA organisms compared to that from the use of mupirocin in vitro. Because antibodies may affect enzyme function, we tested antibodies developed after repeated ClyS exposure for their effect on ClyS killing ability. Our results showed no inhibition of ClyS activity at various antibody titers. These data demonstrate the potential of developing ClyS as a novel class of topical antimicrobial agents specific to staphylococcus. SN - 1098-6596 UR - https://www.unboundmedicine.com/medline/citation/21098252/A_novel_chimeric_lysin_shows_superiority_to_mupirocin_for_skin_decolonization_of_methicillin_resistant_and__sensitive_Staphylococcus_aureus_strains_ L2 - http://aac.asm.org/cgi/pmidlookup?view=long&pmid=21098252 DB - PRIME DP - Unbound Medicine ER -