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Intrathymic radioresistant stem cells follow an IL-2/IL-2R pathway during thymic regeneration after sublethal irradiation.
J Immunol. 1990 May 15; 144(10):3736-40.JI

Abstract

Sublethally irradiated mice undergo thymic regeneration which follows a phenotypic pattern of events similar to that observed during normal fetal development. Thymic regeneration after irradiation is the product of a limited pool of intrathymic radioresistant stem cells undergoing simultaneous differentiation. We show that in this model of T cell development, thymic regeneration follows a pathway in which the IL-2R is transiently expressed on CD4-/CD8- cells. IL-2R expression occurred during the exponential growth period of thymic regeneration, and IL-2R blocking prevented this explosive growth. Flow cytometry analysis revealed that the IL-2R blockade affected primarily the development of the immature CD3-/CD4-/CD8- (triple negative) cells and their ability to generate CD3+/CD4+/CD8+ or CD3+/CD4+/CD8- and CD3+/CD4-/CD8+ thymocytes. Thus, our findings demonstrate that blocking of the IL-2R resulted in an arrest in proliferation and differentiation by intrathymic radioresistant stem cells, indicating that the IL-2/IL-2R pathway is necessary for the expansion of immature triple negative T cells.

Authors+Show Affiliations

Biological Response Modifiers Program, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2110211

Citation

Zúñiga-Pflücker, J C., and A M. Kruisbeek. "Intrathymic Radioresistant Stem Cells Follow an IL-2/IL-2R Pathway During Thymic Regeneration After Sublethal Irradiation." Journal of Immunology (Baltimore, Md. : 1950), vol. 144, no. 10, 1990, pp. 3736-40.
Zúñiga-Pflücker JC, Kruisbeek AM. Intrathymic radioresistant stem cells follow an IL-2/IL-2R pathway during thymic regeneration after sublethal irradiation. J Immunol. 1990;144(10):3736-40.
Zúñiga-Pflücker, J. C., & Kruisbeek, A. M. (1990). Intrathymic radioresistant stem cells follow an IL-2/IL-2R pathway during thymic regeneration after sublethal irradiation. Journal of Immunology (Baltimore, Md. : 1950), 144(10), 3736-40.
Zúñiga-Pflücker JC, Kruisbeek AM. Intrathymic Radioresistant Stem Cells Follow an IL-2/IL-2R Pathway During Thymic Regeneration After Sublethal Irradiation. J Immunol. 1990 May 15;144(10):3736-40. PubMed PMID: 2110211.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intrathymic radioresistant stem cells follow an IL-2/IL-2R pathway during thymic regeneration after sublethal irradiation. AU - Zúñiga-Pflücker,J C, AU - Kruisbeek,A M, PY - 1990/5/15/pubmed PY - 1990/5/15/medline PY - 1990/5/15/entrez SP - 3736 EP - 40 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 144 IS - 10 N2 - Sublethally irradiated mice undergo thymic regeneration which follows a phenotypic pattern of events similar to that observed during normal fetal development. Thymic regeneration after irradiation is the product of a limited pool of intrathymic radioresistant stem cells undergoing simultaneous differentiation. We show that in this model of T cell development, thymic regeneration follows a pathway in which the IL-2R is transiently expressed on CD4-/CD8- cells. IL-2R expression occurred during the exponential growth period of thymic regeneration, and IL-2R blocking prevented this explosive growth. Flow cytometry analysis revealed that the IL-2R blockade affected primarily the development of the immature CD3-/CD4-/CD8- (triple negative) cells and their ability to generate CD3+/CD4+/CD8+ or CD3+/CD4+/CD8- and CD3+/CD4-/CD8+ thymocytes. Thus, our findings demonstrate that blocking of the IL-2R resulted in an arrest in proliferation and differentiation by intrathymic radioresistant stem cells, indicating that the IL-2/IL-2R pathway is necessary for the expansion of immature triple negative T cells. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/2110211/Intrathymic_radioresistant_stem_cells_follow_an_IL_2/IL_2R_pathway_during_thymic_regeneration_after_sublethal_irradiation_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=2110211 DB - PRIME DP - Unbound Medicine ER -