Tags

Type your tag names separated by a space and hit enter

BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei.
PLoS One. 2010 Nov 12; 5(11):e15413.Plos

Abstract

A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei.

Authors+Show Affiliations

Translational Genomics Research Institute, Flagstaff, Arizona, United States of America. jbowers@tgen.orgNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

21103048

Citation

Bowers, Jolene R., et al. "BurkDiff: a Real-time PCR Allelic Discrimination Assay for Burkholderia Pseudomallei and B. Mallei." PloS One, vol. 5, no. 11, 2010, pp. e15413.
Bowers JR, Engelthaler DM, Ginther JL, et al. BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei. PLoS One. 2010;5(11):e15413.
Bowers, J. R., Engelthaler, D. M., Ginther, J. L., Pearson, T., Peacock, S. J., Tuanyok, A., Wagner, D. M., Currie, B. J., & Keim, P. S. (2010). BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei. PloS One, 5(11), e15413. https://doi.org/10.1371/journal.pone.0015413
Bowers JR, et al. BurkDiff: a Real-time PCR Allelic Discrimination Assay for Burkholderia Pseudomallei and B. Mallei. PLoS One. 2010 Nov 12;5(11):e15413. PubMed PMID: 21103048.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei. AU - Bowers,Jolene R, AU - Engelthaler,David M, AU - Ginther,Jennifer L, AU - Pearson,Talima, AU - Peacock,Sharon J, AU - Tuanyok,Apichai, AU - Wagner,David M, AU - Currie,Bart J, AU - Keim,Paul S, Y1 - 2010/11/12/ PY - 2010/07/26/received PY - 2010/09/12/accepted PY - 2010/11/25/entrez PY - 2010/11/26/pubmed PY - 2011/4/28/medline SP - e15413 EP - e15413 JF - PloS one JO - PLoS One VL - 5 IS - 11 N2 - A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/21103048/BurkDiff:_a_real_time_PCR_allelic_discrimination_assay_for_Burkholderia_pseudomallei_and_B__mallei_ L2 - https://dx.plos.org/10.1371/journal.pone.0015413 DB - PRIME DP - Unbound Medicine ER -