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Neopterin formation and tryptophan degradation by a human myelomonocytic cell line (THP-1) upon cytokine treatment.
Cancer Res 1990; 50(10):2863-7CR

Abstract

Determination of neopterin [D-erythro-6-(1',2',3'-trihydroxypropyl)pterin] in body fluids is a powerful diagnostic tool in a variety of diseases in which activation of cellular immune mechanisms is involved, such as certain malignancies, allograft rejection, and autoimmune and infectious diseases. In vitro, neopterin is released into the supernatant by peripheral blood-derived monocytes/macrophages upon stimulation with gamma-interferon. In parallel, cleavage of tryptophan by indoleamine 2,3-dioxygenase is induced. We report here that the human myelomonocytic cell line THP-1 forms neopterin and degrades tryptophan upon treatment with gamma-interferon. Like in macrophages alpha-interferon and beta-interferon induce these pathways only to a much smaller degree. The action of interferons is enhanced by cotreatment with tumor necrosis factor alpha, lipopolysaccharide, or dexamethasone. gamma-Interferon-induced neopterin formation and indoleamine 2,3-dioxygenase activity are increased by raising extracellular tryptophan concentrations. The pattern of intracellularly formed pteridines upon stimulation with gamma-interferon shows the unique characteristics of human monocytes/macrophages. Neopterin, monapterin, and biopterin are produced in a 50:2:1 ratio. Thus, the THP-1 cell line provides a permanent, easily accessible in vitro system for studying the induction and mechanism of neopterin formation.

Authors+Show Affiliations

Institute for Medical Chemistry and Biochemistry, University of Innsbruck, Austria.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2110500

Citation

Werner-Felmayer, G, et al. "Neopterin Formation and Tryptophan Degradation By a Human Myelomonocytic Cell Line (THP-1) Upon Cytokine Treatment." Cancer Research, vol. 50, no. 10, 1990, pp. 2863-7.
Werner-Felmayer G, Werner ER, Fuchs D, et al. Neopterin formation and tryptophan degradation by a human myelomonocytic cell line (THP-1) upon cytokine treatment. Cancer Res. 1990;50(10):2863-7.
Werner-Felmayer, G., Werner, E. R., Fuchs, D., Hausen, A., Reibnegger, G., & Wachter, H. (1990). Neopterin formation and tryptophan degradation by a human myelomonocytic cell line (THP-1) upon cytokine treatment. Cancer Research, 50(10), pp. 2863-7.
Werner-Felmayer G, et al. Neopterin Formation and Tryptophan Degradation By a Human Myelomonocytic Cell Line (THP-1) Upon Cytokine Treatment. Cancer Res. 1990 May 15;50(10):2863-7. PubMed PMID: 2110500.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neopterin formation and tryptophan degradation by a human myelomonocytic cell line (THP-1) upon cytokine treatment. AU - Werner-Felmayer,G, AU - Werner,E R, AU - Fuchs,D, AU - Hausen,A, AU - Reibnegger,G, AU - Wachter,H, PY - 1990/5/15/pubmed PY - 1990/5/15/medline PY - 1990/5/15/entrez SP - 2863 EP - 7 JF - Cancer research JO - Cancer Res. VL - 50 IS - 10 N2 - Determination of neopterin [D-erythro-6-(1',2',3'-trihydroxypropyl)pterin] in body fluids is a powerful diagnostic tool in a variety of diseases in which activation of cellular immune mechanisms is involved, such as certain malignancies, allograft rejection, and autoimmune and infectious diseases. In vitro, neopterin is released into the supernatant by peripheral blood-derived monocytes/macrophages upon stimulation with gamma-interferon. In parallel, cleavage of tryptophan by indoleamine 2,3-dioxygenase is induced. We report here that the human myelomonocytic cell line THP-1 forms neopterin and degrades tryptophan upon treatment with gamma-interferon. Like in macrophages alpha-interferon and beta-interferon induce these pathways only to a much smaller degree. The action of interferons is enhanced by cotreatment with tumor necrosis factor alpha, lipopolysaccharide, or dexamethasone. gamma-Interferon-induced neopterin formation and indoleamine 2,3-dioxygenase activity are increased by raising extracellular tryptophan concentrations. The pattern of intracellularly formed pteridines upon stimulation with gamma-interferon shows the unique characteristics of human monocytes/macrophages. Neopterin, monapterin, and biopterin are produced in a 50:2:1 ratio. Thus, the THP-1 cell line provides a permanent, easily accessible in vitro system for studying the induction and mechanism of neopterin formation. SN - 0008-5472 UR - https://www.unboundmedicine.com/medline/citation/2110500/Neopterin_formation_and_tryptophan_degradation_by_a_human_myelomonocytic_cell_line__THP_1__upon_cytokine_treatment_ L2 - http://cancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=2110500 DB - PRIME DP - Unbound Medicine ER -