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Identification and isolation of Genotype-I Japanese encephalitis virus from encephalitis patients.
Virol J. 2010 Nov 26; 7:345.VJ

Abstract

Historically, Japanese Encephalitis virus (JEV) genotype III (GIII) has been responsible for human diseases. In recent years, JEV genotype I (GI) has been isolated from mosquitoes collected in numerous countries, but has not been isolated from patients with encephalitis. In this study, we report recovery of JEV GI live virus and identification of JEV GI RNA from cerebrospinal fluid (CSF) of encephalitis patients in JE endemic areas of China. Whole-genome sequencing and molecular phylogenetic analysis of the JEV isolate from the CSF samples was performed. The isolate in this study is highly similar to other JEV GI strains which isolated from mosquitoes at both the nucleotide and deduced amino acid levels. Phylogenetic analysis based on the genomic sequence showed that the isolate belongs to JEV GI, which is consistent with the phylogenetic analysis based on the pre-membrane (PrM) and Glycoprotein genes. As a conclusion, this is the first time to isolate JEV GI strain from CSF samples of encephalitis patients, so continuous survey and evaluate the infectivity and pathogenecity of JEV GI strains are necessary, especially for the JEV GI strains from encephalitis patients. With respect to the latter, because all current JEV vaccines (live and inactivated are derived from JEV GIII strains, future studies should be aimed at investigating and monitoring cross-protection of the human JEV GI isolates against widely used JEV vaccines.

Authors+Show Affiliations

State Key Laboratory for Infectious Disease Prevention and Control (SKLID), Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, PR China. wanglih9755@yahoo.com.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21108846

Citation

Wang, Lihua, et al. "Identification and Isolation of Genotype-I Japanese Encephalitis Virus From Encephalitis Patients." Virology Journal, vol. 7, 2010, p. 345.
Wang L, Fu S, Zhang H, et al. Identification and isolation of Genotype-I Japanese encephalitis virus from encephalitis patients. Virol J. 2010;7:345.
Wang, L., Fu, S., Zhang, H., Ye, X., Yu, D., Deng, Z., Yuan, J., Zhai, Y., Li, M., Lv, Z., Chen, W., Jiang, H., Gao, X., Cao, Y., Wang, H., Tang, Q., & Liang, G. (2010). Identification and isolation of Genotype-I Japanese encephalitis virus from encephalitis patients. Virology Journal, 7, 345. https://doi.org/10.1186/1743-422X-7-345
Wang L, et al. Identification and Isolation of Genotype-I Japanese Encephalitis Virus From Encephalitis Patients. Virol J. 2010 Nov 26;7:345. PubMed PMID: 21108846.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and isolation of Genotype-I Japanese encephalitis virus from encephalitis patients. AU - Wang,Lihua, AU - Fu,Shihong, AU - Zhang,Hailin, AU - Ye,Xufang, AU - Yu,Deshan, AU - Deng,Zhang, AU - Yuan,Jun, AU - Zhai,Yougang, AU - Li,Minghua, AU - Lv,Zhi, AU - Chen,Weixin, AU - Jiang,Hongyue, AU - Gao,Xiaoyan, AU - Cao,Yuxi, AU - Wang,Huanyu, AU - Tang,Qing, AU - Liang,Guodong, Y1 - 2010/11/26/ PY - 2010/09/29/received PY - 2010/11/26/accepted PY - 2010/11/27/entrez PY - 2010/11/27/pubmed PY - 2011/2/1/medline SP - 345 EP - 345 JF - Virology journal JO - Virol J VL - 7 N2 - Historically, Japanese Encephalitis virus (JEV) genotype III (GIII) has been responsible for human diseases. In recent years, JEV genotype I (GI) has been isolated from mosquitoes collected in numerous countries, but has not been isolated from patients with encephalitis. In this study, we report recovery of JEV GI live virus and identification of JEV GI RNA from cerebrospinal fluid (CSF) of encephalitis patients in JE endemic areas of China. Whole-genome sequencing and molecular phylogenetic analysis of the JEV isolate from the CSF samples was performed. The isolate in this study is highly similar to other JEV GI strains which isolated from mosquitoes at both the nucleotide and deduced amino acid levels. Phylogenetic analysis based on the genomic sequence showed that the isolate belongs to JEV GI, which is consistent with the phylogenetic analysis based on the pre-membrane (PrM) and Glycoprotein genes. As a conclusion, this is the first time to isolate JEV GI strain from CSF samples of encephalitis patients, so continuous survey and evaluate the infectivity and pathogenecity of JEV GI strains are necessary, especially for the JEV GI strains from encephalitis patients. With respect to the latter, because all current JEV vaccines (live and inactivated are derived from JEV GIII strains, future studies should be aimed at investigating and monitoring cross-protection of the human JEV GI isolates against widely used JEV vaccines. SN - 1743-422X UR - https://www.unboundmedicine.com/medline/citation/21108846/Identification_and_isolation_of_Genotype_I_Japanese_encephalitis_virus_from_encephalitis_patients_ DB - PRIME DP - Unbound Medicine ER -