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Immunopathology of experimental Brucella abortus strain 19 infection of the genitalia of bulls.
Vet Immunol Immunopathol. 1990 Mar; 24(3):235-46.VI

Abstract

Antibody responses in serum and semen, and immunoglobulin containing cell (ICC) populations in the genitalia of bulls were compared after inoculating Brucella abortus strain 19 into the seminal vesicles of two bulls (ISV route) and into testes in two other bulls (IT route). Bulls seroconverted as early as 1 week post-infection (PI). Peak serum titres as determined by the serum agglutination test (SAT), complement fixation test (CFT) and ELISA occurred at PI weeks 3, 4 and 5 respectively. Highest titres were in IT inoculated bulls. Seminal antibodies against B. arbotus S19 were demonstrated from 2 weeks PI by both the SAT and the Rose Bengal Test (RBT) and highest titres occurred at PI weeks 3 and 4. Examination of immunoglobulins (Ig) in semen, however, revealed no significant differences of Ig isotypes between infected and control animals at any examination time. When bulls were killed at 7 weeks PI, quantitation of ICC in genital sections stained by the peroxidase-anti-peroxidase method revealed an overwhelming predominance of IgG containing cells in inflamed organs. In all cases IgG1- and IgG2-containing cells were prevalent, and present in approximately equal numbers. IgA-containing cells were second in prevalence in inflamed tissues while IgM cells were always in low percentage. High prevalence of ICC in infected genitalia, associated with elevated specific seminal antibodies but not with increased seminal Ig indicates that most Ig remains localised in tissues and is not transferred into genital secretions.

Authors+Show Affiliations

Graduate School of Tropical Veterinary Science, James Cook University of North Queensland, Townsville, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2111057

Citation

Campero, C M., et al. "Immunopathology of Experimental Brucella Abortus Strain 19 Infection of the Genitalia of Bulls." Veterinary Immunology and Immunopathology, vol. 24, no. 3, 1990, pp. 235-46.
Campero CM, Ladds PW, Hoffmann D, et al. Immunopathology of experimental Brucella abortus strain 19 infection of the genitalia of bulls. Vet Immunol Immunopathol. 1990;24(3):235-46.
Campero, C. M., Ladds, P. W., Hoffmann, D., Duffield, B., Watson, D., & Fordyce, G. (1990). Immunopathology of experimental Brucella abortus strain 19 infection of the genitalia of bulls. Veterinary Immunology and Immunopathology, 24(3), 235-46.
Campero CM, et al. Immunopathology of Experimental Brucella Abortus Strain 19 Infection of the Genitalia of Bulls. Vet Immunol Immunopathol. 1990;24(3):235-46. PubMed PMID: 2111057.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunopathology of experimental Brucella abortus strain 19 infection of the genitalia of bulls. AU - Campero,C M, AU - Ladds,P W, AU - Hoffmann,D, AU - Duffield,B, AU - Watson,D, AU - Fordyce,G, PY - 1990/3/1/pubmed PY - 1990/3/1/medline PY - 1990/3/1/entrez SP - 235 EP - 46 JF - Veterinary immunology and immunopathology JO - Vet. Immunol. Immunopathol. VL - 24 IS - 3 N2 - Antibody responses in serum and semen, and immunoglobulin containing cell (ICC) populations in the genitalia of bulls were compared after inoculating Brucella abortus strain 19 into the seminal vesicles of two bulls (ISV route) and into testes in two other bulls (IT route). Bulls seroconverted as early as 1 week post-infection (PI). Peak serum titres as determined by the serum agglutination test (SAT), complement fixation test (CFT) and ELISA occurred at PI weeks 3, 4 and 5 respectively. Highest titres were in IT inoculated bulls. Seminal antibodies against B. arbotus S19 were demonstrated from 2 weeks PI by both the SAT and the Rose Bengal Test (RBT) and highest titres occurred at PI weeks 3 and 4. Examination of immunoglobulins (Ig) in semen, however, revealed no significant differences of Ig isotypes between infected and control animals at any examination time. When bulls were killed at 7 weeks PI, quantitation of ICC in genital sections stained by the peroxidase-anti-peroxidase method revealed an overwhelming predominance of IgG containing cells in inflamed organs. In all cases IgG1- and IgG2-containing cells were prevalent, and present in approximately equal numbers. IgA-containing cells were second in prevalence in inflamed tissues while IgM cells were always in low percentage. High prevalence of ICC in infected genitalia, associated with elevated specific seminal antibodies but not with increased seminal Ig indicates that most Ig remains localised in tissues and is not transferred into genital secretions. SN - 0165-2427 UR - https://www.unboundmedicine.com/medline/citation/2111057/Immunopathology_of_experimental_Brucella_abortus_strain_19_infection_of_the_genitalia_of_bulls_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0165-2427(90)90039-U DB - PRIME DP - Unbound Medicine ER -