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Pulsed multi-wavelength excitation using fiber-in-capillary light emitting diode induced fluorescence detection in capillary electrophoresis.
Talanta. 2010 Dec 15; 83(2):521-6.T

Abstract

A novel instrument was developed using a multi-wavelength pulsed LED array with in-column optic-fiber induced fluorescence detection by capillary electrophoresis. The light from 2 different wavelength LEDs (450 nm and 480 nm) was pulsed for short intervals at high intensity. The beam from each LED was collimated and reshaped with the gradient index (GRIN) lens group to achieve a highly effective coupling between LED light source and an optical fiber. The optical fiber was placed inside the capillary for in-capillary LED-induced fluorescence detection. The advantages of this system were validated by the simultaneous determination of vitamin B2 and fluorescein. Detection limits for vitamin B2 and fluorescein were estimated to be 5 nM and 0.29 nM (S/N=3), respectively. The relative standard deviations (RSDs, n=6) of the both compounds for migration time and peak area were better than 0.83%, 2.20% and 1.21%, 2.75%, respectively. The method was applied to the determination of vitamin B2 in commercial tablets and fluorescein in fluorescein sodium injection and the recoveries obtained were in the range of 96.6-102.0% and 99.9-102.8%, respectively. It was also applied to human serum, where the recoveries were found to be in the range of 94.4-97.0% and 92.6-96.4%, respectively. The system has been successfully applied in separation and determination of the both biological samples with acceptable analytical performance.

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Authors+Show Affiliations

Huo F
College of Chemistry and College of Chemical Engineering, Sichuan University, Chengdu 610065, PR China.
Yuan H
No affiliation info available
Yang X
No affiliation info available
Breadmore MC
No affiliation info available
Xiao D
No affiliation info available

MeSH

Blood Chemical AnalysisChemistry Techniques, AnalyticalElectrophoresis, CapillaryEquipment DesignFiber Optic TechnologyFluoresceinHumansLightOptical FibersOptics and PhotonicsReproducibility of ResultsRiboflavinSpectrometry, FluorescenceTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21111168

Citation

Huo, Feng, et al. "Pulsed Multi-wavelength Excitation Using Fiber-in-capillary Light Emitting Diode Induced Fluorescence Detection in Capillary Electrophoresis." Talanta, vol. 83, no. 2, 2010, pp. 521-6.
Huo F, Yuan H, Yang X, et al. Pulsed multi-wavelength excitation using fiber-in-capillary light emitting diode induced fluorescence detection in capillary electrophoresis. Talanta. 2010;83(2):521-6.
Huo, F., Yuan, H., Yang, X., Breadmore, M. C., & Xiao, D. (2010). Pulsed multi-wavelength excitation using fiber-in-capillary light emitting diode induced fluorescence detection in capillary electrophoresis. Talanta, 83(2), 521-6. https://doi.org/10.1016/j.talanta.2010.09.047
Huo F, et al. Pulsed Multi-wavelength Excitation Using Fiber-in-capillary Light Emitting Diode Induced Fluorescence Detection in Capillary Electrophoresis. Talanta. 2010 Dec 15;83(2):521-6. PubMed PMID: 21111168.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pulsed multi-wavelength excitation using fiber-in-capillary light emitting diode induced fluorescence detection in capillary electrophoresis. AU - Huo,Feng, AU - Yuan,Hongyan, AU - Yang,Xiupei, AU - Breadmore,Michael C, AU - Xiao,Dan, Y1 - 2010/10/01/ PY - 2010/06/14/received PY - 2010/09/20/revised PY - 2010/09/27/accepted PY - 2010/11/30/entrez PY - 2010/11/30/pubmed PY - 2011/4/13/medline SP - 521 EP - 6 JF - Talanta JO - Talanta VL - 83 IS - 2 N2 - A novel instrument was developed using a multi-wavelength pulsed LED array with in-column optic-fiber induced fluorescence detection by capillary electrophoresis. The light from 2 different wavelength LEDs (450 nm and 480 nm) was pulsed for short intervals at high intensity. The beam from each LED was collimated and reshaped with the gradient index (GRIN) lens group to achieve a highly effective coupling between LED light source and an optical fiber. The optical fiber was placed inside the capillary for in-capillary LED-induced fluorescence detection. The advantages of this system were validated by the simultaneous determination of vitamin B2 and fluorescein. Detection limits for vitamin B2 and fluorescein were estimated to be 5 nM and 0.29 nM (S/N=3), respectively. The relative standard deviations (RSDs, n=6) of the both compounds for migration time and peak area were better than 0.83%, 2.20% and 1.21%, 2.75%, respectively. The method was applied to the determination of vitamin B2 in commercial tablets and fluorescein in fluorescein sodium injection and the recoveries obtained were in the range of 96.6-102.0% and 99.9-102.8%, respectively. It was also applied to human serum, where the recoveries were found to be in the range of 94.4-97.0% and 92.6-96.4%, respectively. The system has been successfully applied in separation and determination of the both biological samples with acceptable analytical performance. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/21111168/Pulsed_multi_wavelength_excitation_using_fiber_in_capillary_light_emitting_diode_induced_fluorescence_detection_in_capillary_electrophoresis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(10)00761-7 DB - PRIME DP - Unbound Medicine ER -