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Standard practice for cell sorting in a BSL-3 facility.
Methods Mol Biol. 2011; 699:449-69.MM

Abstract

Over the past decade, there has been a rapid growth in the number of BSL-3 and BSL-4 laboratories in the USA and an increase in demand for infectious cell sorting in BSL-3 laboratories. In 2007, the International Society for Advancement of Cytometry (ISAC) Biosafety Committee published standards for the sorting of unfixed cells and is an important resource for biosafety procedures when performing infectious cell sorting. Following a careful risk assessment, if it is determined that a cell sorter must be located within a BSL-3 laboratory, there are a variety of factors to be considered prior to the establishment of the laboratory. This chapter outlines procedures for infectious cell sorting in a BSL-3 environment to facilitate the establishment and safe operation of a BSL-3 cell sorting laboratory. Subjects covered include containment verification, remote operation, disinfection, personal protective equipment (PPE), and instrument-specific modifications for enhanced aerosol evacuation.

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Authors+Show Affiliations

Perfetto SP
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
Ambrozak DR
No affiliation info available
Nguyen R
No affiliation info available
Roederer M
No affiliation info available
Koup RA
No affiliation info available
Holmes KL
No affiliation info available

MeSH

AerosolsContainment of BiohazardsFacility Regulation and ControlFlow CytometryLaboratoriesProtective DevicesSafety Management

Pub Type(s)

Journal Article
Research Support, N.I.H., Intramural

Language

eng

PubMed ID

21116997

Citation

Perfetto, Stephen P., et al. "Standard Practice for Cell Sorting in a BSL-3 Facility." Methods in Molecular Biology (Clifton, N.J.), vol. 699, 2011, pp. 449-69.
Perfetto SP, Ambrozak DR, Nguyen R, et al. Standard practice for cell sorting in a BSL-3 facility. Methods Mol Biol. 2011;699:449-69.
Perfetto, S. P., Ambrozak, D. R., Nguyen, R., Roederer, M., Koup, R. A., & Holmes, K. L. (2011). Standard practice for cell sorting in a BSL-3 facility. Methods in Molecular Biology (Clifton, N.J.), 699, 449-69. https://doi.org/10.1007/978-1-61737-950-5_22
Perfetto SP, et al. Standard Practice for Cell Sorting in a BSL-3 Facility. Methods Mol Biol. 2011;699:449-69. PubMed PMID: 21116997.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Standard practice for cell sorting in a BSL-3 facility. AU - Perfetto,Stephen P, AU - Ambrozak,David R, AU - Nguyen,Richard, AU - Roederer,Mario, AU - Koup,Richard A, AU - Holmes,Kevin L, PY - 2010/12/1/entrez PY - 2010/12/1/pubmed PY - 2011/5/24/medline SP - 449 EP - 69 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 699 N2 - Over the past decade, there has been a rapid growth in the number of BSL-3 and BSL-4 laboratories in the USA and an increase in demand for infectious cell sorting in BSL-3 laboratories. In 2007, the International Society for Advancement of Cytometry (ISAC) Biosafety Committee published standards for the sorting of unfixed cells and is an important resource for biosafety procedures when performing infectious cell sorting. Following a careful risk assessment, if it is determined that a cell sorter must be located within a BSL-3 laboratory, there are a variety of factors to be considered prior to the establishment of the laboratory. This chapter outlines procedures for infectious cell sorting in a BSL-3 environment to facilitate the establishment and safe operation of a BSL-3 cell sorting laboratory. Subjects covered include containment verification, remote operation, disinfection, personal protective equipment (PPE), and instrument-specific modifications for enhanced aerosol evacuation. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/21116997/Standard_practice_for_cell_sorting_in_a_BSL_3_facility_ L2 - https://dx.doi.org/10.1007/978-1-61737-950-5_22 DB - PRIME DP - Unbound Medicine ER -