Perfetto, Stephen P., et al. "Standard Practice for Cell Sorting in a BSL-3 Facility." Methods in Molecular Biology (Clifton, N.J.), vol. 699, 2011, pp. 449-69.
Perfetto SP, Ambrozak DR, Nguyen R, et al. Standard practice for cell sorting in a BSL-3 facility. Methods Mol Biol. 2011;699:449-69.
Perfetto, S. P., Ambrozak, D. R., Nguyen, R., Roederer, M., Koup, R. A., & Holmes, K. L. (2011). Standard practice for cell sorting in a BSL-3 facility. Methods in Molecular Biology (Clifton, N.J.), 699, 449-69. https://doi.org/10.1007/978-1-61737-950-5_22
Perfetto SP, et al. Standard Practice for Cell Sorting in a BSL-3 Facility. Methods Mol Biol. 2011;699:449-69. PubMed PMID: 21116997.
TY - JOUR
T1 - Standard practice for cell sorting in a BSL-3 facility.
AU - Perfetto,Stephen P,
AU - Ambrozak,David R,
AU - Nguyen,Richard,
AU - Roederer,Mario,
AU - Koup,Richard A,
AU - Holmes,Kevin L,
PY - 2010/12/1/entrez
PY - 2010/12/1/pubmed
PY - 2011/5/24/medline
SP - 449
EP - 69
JF - Methods in molecular biology (Clifton, N.J.)
JO - Methods Mol Biol
VL - 699
N2 - Over the past decade, there has been a rapid growth in the number of BSL-3 and BSL-4 laboratories in the USA and an increase in demand for infectious cell sorting in BSL-3 laboratories. In 2007, the International Society for Advancement of Cytometry (ISAC) Biosafety Committee published standards for the sorting of unfixed cells and is an important resource for biosafety procedures when performing infectious cell sorting. Following a careful risk assessment, if it is determined that a cell sorter must be located within a BSL-3 laboratory, there are a variety of factors to be considered prior to the establishment of the laboratory. This chapter outlines procedures for infectious cell sorting in a BSL-3 environment to facilitate the establishment and safe operation of a BSL-3 cell sorting laboratory. Subjects covered include containment verification, remote operation, disinfection, personal protective equipment (PPE), and instrument-specific modifications for enhanced aerosol evacuation.
SN - 1940-6029
UR - https://www.unboundmedicine.com/medline/citation/21116997/Standard_practice_for_cell_sorting_in_a_BSL_3_facility_
L2 - https://dx.doi.org/10.1007/978-1-61737-950-5_22
DB - PRIME
DP - Unbound Medicine