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New system for multilocus variable-number tandem-repeat analysis of the enterohemorrhagic Escherichia coli strains belonging to three major serogroups: O157, O26, and O111.
Microbiol Immunol. 2010 Oct; 54(10):569-77.MI

Abstract

Enterohemorrhagic Escherichia coli (EHEC), a food- and waterborne pathogen, causes diarrhea, hemorrhagic colitis, and life-threatening HUS. MLVA is a newly developed and widely accepted genotyping tool. An MLVA system for EHEC O157 involving nine genomic loci has already been established. However, the present study revealed that the above-mentioned MLVA system cannot analyze EHEC O26 and O111 isolates-the second and third most dominant EHEC serogroups in Japan, respectively. Therefore, with several modifications to the O157 system and the use of nine additional loci, we developed an expanded MLVA system applicable to EHEC O26, O111, and O157. Our MLVA system had a relatively high resolution power for each of the three serogroups: Simpson's index of diversity was 0.991 (95% CI = 0.989-0.993), 0.988 (95% CI, 0.986-0.990), and 0.986 (95% CI, 0.979-0.993) for O26, O111, and O157, respectively. This system also detected outbreak-related isolates; the isolates collected during each of the 12 O26 and O111 outbreaks formed unique clusters, and most of the repeat copy numbers among the isolates collected during the same outbreak exhibited no or single-locus variations. These results were comparable to those of cluster analyses based on PFGE profiles. Therefore, our system can complement PFGE analysis-the current golden method. Because EHEC strains of three major serogroups can be rapidly analyzed on a single platform with our expanded MLVA system, this system could be widely used in molecular epidemiological studies of EHEC infections.

Authors+Show Affiliations

Department of Bacteriology I, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan. izumiya@nih.go.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21118294

Citation

Izumiya, Hidemasa, et al. "New System for Multilocus Variable-number Tandem-repeat Analysis of the Enterohemorrhagic Escherichia Coli Strains Belonging to Three Major Serogroups: O157, O26, and O111." Microbiology and Immunology, vol. 54, no. 10, 2010, pp. 569-77.
Izumiya H, Pei Y, Terajima J, et al. New system for multilocus variable-number tandem-repeat analysis of the enterohemorrhagic Escherichia coli strains belonging to three major serogroups: O157, O26, and O111. Microbiol Immunol. 2010;54(10):569-77.
Izumiya, H., Pei, Y., Terajima, J., Ohnishi, M., Hayashi, T., Iyoda, S., & Watanabe, H. (2010). New system for multilocus variable-number tandem-repeat analysis of the enterohemorrhagic Escherichia coli strains belonging to three major serogroups: O157, O26, and O111. Microbiology and Immunology, 54(10), 569-77. https://doi.org/10.1111/j.1348-0421.2010.00252.x
Izumiya H, et al. New System for Multilocus Variable-number Tandem-repeat Analysis of the Enterohemorrhagic Escherichia Coli Strains Belonging to Three Major Serogroups: O157, O26, and O111. Microbiol Immunol. 2010;54(10):569-77. PubMed PMID: 21118294.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - New system for multilocus variable-number tandem-repeat analysis of the enterohemorrhagic Escherichia coli strains belonging to three major serogroups: O157, O26, and O111. AU - Izumiya,Hidemasa, AU - Pei,Yingxin, AU - Terajima,Jun, AU - Ohnishi,Makoto, AU - Hayashi,Tetsuya, AU - Iyoda,Sunao, AU - Watanabe,Haruo, PY - 2010/12/2/entrez PY - 2010/12/2/pubmed PY - 2010/12/22/medline SP - 569 EP - 77 JF - Microbiology and immunology JO - Microbiol Immunol VL - 54 IS - 10 N2 - Enterohemorrhagic Escherichia coli (EHEC), a food- and waterborne pathogen, causes diarrhea, hemorrhagic colitis, and life-threatening HUS. MLVA is a newly developed and widely accepted genotyping tool. An MLVA system for EHEC O157 involving nine genomic loci has already been established. However, the present study revealed that the above-mentioned MLVA system cannot analyze EHEC O26 and O111 isolates-the second and third most dominant EHEC serogroups in Japan, respectively. Therefore, with several modifications to the O157 system and the use of nine additional loci, we developed an expanded MLVA system applicable to EHEC O26, O111, and O157. Our MLVA system had a relatively high resolution power for each of the three serogroups: Simpson's index of diversity was 0.991 (95% CI = 0.989-0.993), 0.988 (95% CI, 0.986-0.990), and 0.986 (95% CI, 0.979-0.993) for O26, O111, and O157, respectively. This system also detected outbreak-related isolates; the isolates collected during each of the 12 O26 and O111 outbreaks formed unique clusters, and most of the repeat copy numbers among the isolates collected during the same outbreak exhibited no or single-locus variations. These results were comparable to those of cluster analyses based on PFGE profiles. Therefore, our system can complement PFGE analysis-the current golden method. Because EHEC strains of three major serogroups can be rapidly analyzed on a single platform with our expanded MLVA system, this system could be widely used in molecular epidemiological studies of EHEC infections. SN - 0385-5600 UR - https://www.unboundmedicine.com/medline/citation/21118294/New_system_for_multilocus_variable_number_tandem_repeat_analysis_of_the_enterohemorrhagic_Escherichia_coli_strains_belonging_to_three_major_serogroups:_O157_O26_and_O111_ L2 - https://doi.org/10.1111/j.1348-0421.2010.00252.x DB - PRIME DP - Unbound Medicine ER -