Tags

Type your tag names separated by a space and hit enter

Surface-enhanced Raman scattering detection of DNA derived from the west nile virus genome using magnetic capture of Raman-active gold nanoparticles.
Anal Chem. 2011 Jan 01; 83(1):254-60.AC

Abstract

A model paramagnetic nanoparticle (MNP) assay is demonstrated for surface-enhanced Raman scattering (SERS) detection of DNA oligonucleotides derived from the West Nile virus (WNV) genome. Detection is based on the capture of WNV target sequences by hybridization with complementary oligonucleotide probes covalently linked to fabricated MNPs and Raman reporter tag-conjugated gold nanoparticles (GNPs) and the subsequent removal of GNP-WNV target sequence-MNP hybridization complexes from solution by an externally applied magnetic source. Laser excitation of the pelleted material provided a signature SERS spectrum which is diagnostic for the reporter, 5,5'-dithiobis(succinimidy-2-nitrobenzoate) (DSNB), and restricted to hybridization reactions containing WNV target sequences. Hybridizations containing dilutions of the target oligonucleotide were characterized by a reduction in the intensification of the spectral peaks accorded to the SERS signaling of DSNB, and the limit of detection for target sequence in buffer was 10 pM. Due to the short hybridization times required to conduct the assay and ease with which reproducible Raman spectra can be acquired, the assay is amenable to adaptation within a portable, user-friendly Raman detection platform for nucleic acids.

Authors+Show Affiliations

Department of Chemical and Petroleum Engineering, University of Wyoming, Laramie, Wyoming 82071, United States.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

21121693

Citation

Zhang, Hao, et al. "Surface-enhanced Raman Scattering Detection of DNA Derived From the West Nile Virus Genome Using Magnetic Capture of Raman-active Gold Nanoparticles." Analytical Chemistry, vol. 83, no. 1, 2011, pp. 254-60.
Zhang H, Harpster MH, Park HJ, et al. Surface-enhanced Raman scattering detection of DNA derived from the west nile virus genome using magnetic capture of Raman-active gold nanoparticles. Anal Chem. 2011;83(1):254-60.
Zhang, H., Harpster, M. H., Park, H. J., Johnson, P. A., & Wilson, W. C. (2011). Surface-enhanced Raman scattering detection of DNA derived from the west nile virus genome using magnetic capture of Raman-active gold nanoparticles. Analytical Chemistry, 83(1), 254-60. https://doi.org/10.1021/ac1023843
Zhang H, et al. Surface-enhanced Raman Scattering Detection of DNA Derived From the West Nile Virus Genome Using Magnetic Capture of Raman-active Gold Nanoparticles. Anal Chem. 2011 Jan 1;83(1):254-60. PubMed PMID: 21121693.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface-enhanced Raman scattering detection of DNA derived from the west nile virus genome using magnetic capture of Raman-active gold nanoparticles. AU - Zhang,Hao, AU - Harpster,Mark H, AU - Park,Hee Joon, AU - Johnson,Patrick A, AU - Wilson,William C, Y1 - 2010/12/01/ PY - 2010/12/3/entrez PY - 2010/12/3/pubmed PY - 2011/3/25/medline SP - 254 EP - 60 JF - Analytical chemistry JO - Anal Chem VL - 83 IS - 1 N2 - A model paramagnetic nanoparticle (MNP) assay is demonstrated for surface-enhanced Raman scattering (SERS) detection of DNA oligonucleotides derived from the West Nile virus (WNV) genome. Detection is based on the capture of WNV target sequences by hybridization with complementary oligonucleotide probes covalently linked to fabricated MNPs and Raman reporter tag-conjugated gold nanoparticles (GNPs) and the subsequent removal of GNP-WNV target sequence-MNP hybridization complexes from solution by an externally applied magnetic source. Laser excitation of the pelleted material provided a signature SERS spectrum which is diagnostic for the reporter, 5,5'-dithiobis(succinimidy-2-nitrobenzoate) (DSNB), and restricted to hybridization reactions containing WNV target sequences. Hybridizations containing dilutions of the target oligonucleotide were characterized by a reduction in the intensification of the spectral peaks accorded to the SERS signaling of DSNB, and the limit of detection for target sequence in buffer was 10 pM. Due to the short hybridization times required to conduct the assay and ease with which reproducible Raman spectra can be acquired, the assay is amenable to adaptation within a portable, user-friendly Raman detection platform for nucleic acids. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/21121693/Surface_enhanced_Raman_scattering_detection_of_DNA_derived_from_the_west_nile_virus_genome_using_magnetic_capture_of_Raman_active_gold_nanoparticles_ L2 - https://doi.org/10.1021/ac1023843 DB - PRIME DP - Unbound Medicine ER -