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The 5' and 3' downstream AUG region elements are required for mosquito-borne flavivirus RNA replication.
J Virol. 2011 Feb; 85(4):1900-5.JV

Abstract

Flaviviruses require complementarity between the 5' and 3' ends of the genome for RNA replication. For mosquito-borne flaviviruses, the cyclization sequences (CS) and upstream of AUG region (UAR) elements at the genomic termini are necessary for viral RNA replication, and a third motif, the downstream of AUG region (DAR), was recently designated for dengue virus. The 3' DAR sequence is also part of a hairpin (HP-3'SL), and both complementarity between 5' and 3' DAR motifs and formation of the HP-3'SL in the absence of the 5' end are conserved among mosquito-borne flaviviruses. Using West Nile virus as a model, we demonstrate that 5'-3' DAR complementarity and HP-3'SL formation are essential for viral RNA replication.

Authors+Show Affiliations

Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, Berkeley, California 94720-7354, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

21123391

Citation

Friebe, Peter, et al. "The 5' and 3' Downstream AUG Region Elements Are Required for Mosquito-borne Flavivirus RNA Replication." Journal of Virology, vol. 85, no. 4, 2011, pp. 1900-5.
Friebe P, Shi PY, Harris E. The 5' and 3' downstream AUG region elements are required for mosquito-borne flavivirus RNA replication. J Virol. 2011;85(4):1900-5.
Friebe, P., Shi, P. Y., & Harris, E. (2011). The 5' and 3' downstream AUG region elements are required for mosquito-borne flavivirus RNA replication. Journal of Virology, 85(4), 1900-5. https://doi.org/10.1128/JVI.02037-10
Friebe P, Shi PY, Harris E. The 5' and 3' Downstream AUG Region Elements Are Required for Mosquito-borne Flavivirus RNA Replication. J Virol. 2011;85(4):1900-5. PubMed PMID: 21123391.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The 5' and 3' downstream AUG region elements are required for mosquito-borne flavivirus RNA replication. AU - Friebe,Peter, AU - Shi,Pei-Yong, AU - Harris,Eva, Y1 - 2010/12/01/ PY - 2010/12/3/entrez PY - 2010/12/3/pubmed PY - 2011/3/8/medline SP - 1900 EP - 5 JF - Journal of virology JO - J. Virol. VL - 85 IS - 4 N2 - Flaviviruses require complementarity between the 5' and 3' ends of the genome for RNA replication. For mosquito-borne flaviviruses, the cyclization sequences (CS) and upstream of AUG region (UAR) elements at the genomic termini are necessary for viral RNA replication, and a third motif, the downstream of AUG region (DAR), was recently designated for dengue virus. The 3' DAR sequence is also part of a hairpin (HP-3'SL), and both complementarity between 5' and 3' DAR motifs and formation of the HP-3'SL in the absence of the 5' end are conserved among mosquito-borne flaviviruses. Using West Nile virus as a model, we demonstrate that 5'-3' DAR complementarity and HP-3'SL formation are essential for viral RNA replication. SN - 1098-5514 UR - https://www.unboundmedicine.com/medline/citation/21123391/The_5'_and_3'_downstream_AUG_region_elements_are_required_for_mosquito_borne_flavivirus_RNA_replication_ L2 - http://jvi.asm.org/cgi/pmidlookup?view=long&pmid=21123391 DB - PRIME DP - Unbound Medicine ER -