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SERS-based sandwich immunoassay using antibody coated magnetic nanoparticles for Escherichia coli enumeration.
Analyst. 2011 Feb 21; 136(4):740-8.A

Abstract

A method combining immunomagnetic separation (IMS) and surface-enhanced Raman scattering (SERS) was developed to enumerate Escherichia coli (E. coli). Gold-coated magnetic spherical nanoparticles were prepared by immobilizing biotin-labeled anti-E. coli antibodies onto avidin-coated magnetic nanoparticles and used in the separation and concentration of the E. coli cells. Raman labels have been constructed using rod shaped gold nanoparticles coated with 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB) and subsequently with a molecular recognizer. Then DTNB-labeled gold nanorods were interacted with gold-coated magnetic spherical nanoparticle-antibody-E. coli complex. The capture efficiency and calibration graphs were obtained and examined in different E. coli concentrations (10(1)-10(7) cfu mL(-1)). The correlation between the concentration of bacteria and SERS signal was found to be linear within the range of 10(1)-10(4) cfu mL(-1) (R(2) = 0.992). The limit of detection (LOD) and limit of quantification (LOQ) values of the developed method were found to be 8 and 24 cfu mL(-1), respectively. The selectivity of the developed immunoassay was examined with Enterobacter aerogenes, Enterobacter dissolvens, and Salmonella enteriditis which did not produce any significant response. The ability of the immunoassay to detect E. coli in real water samples was also investigated and the results were compared with the experimental results from plate-counting methods. There was no significant difference between the methods that were compared (p > 0.05). This method is rapid and sensitive to target organisms with a total analysis time of less than 70 min.

Authors+Show Affiliations

Department of Food Engineering, Faculty of Engineering, Hacettepe University, Beytepe Ankara, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21125089

Citation

Guven, Burcu, et al. "SERS-based Sandwich Immunoassay Using Antibody Coated Magnetic Nanoparticles for Escherichia Coli Enumeration." The Analyst, vol. 136, no. 4, 2011, pp. 740-8.
Guven B, Basaran-Akgul N, Temur E, et al. SERS-based sandwich immunoassay using antibody coated magnetic nanoparticles for Escherichia coli enumeration. Analyst. 2011;136(4):740-8.
Guven, B., Basaran-Akgul, N., Temur, E., Tamer, U., & Boyaci, I. H. (2011). SERS-based sandwich immunoassay using antibody coated magnetic nanoparticles for Escherichia coli enumeration. The Analyst, 136(4), 740-8. https://doi.org/10.1039/c0an00473a
Guven B, et al. SERS-based Sandwich Immunoassay Using Antibody Coated Magnetic Nanoparticles for Escherichia Coli Enumeration. Analyst. 2011 Feb 21;136(4):740-8. PubMed PMID: 21125089.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SERS-based sandwich immunoassay using antibody coated magnetic nanoparticles for Escherichia coli enumeration. AU - Guven,Burcu, AU - Basaran-Akgul,Nese, AU - Temur,Erhan, AU - Tamer,Ugur, AU - Boyaci,Ismail Hakki, Y1 - 2010/12/01/ PY - 2010/12/3/entrez PY - 2010/12/3/pubmed PY - 2011/5/13/medline SP - 740 EP - 8 JF - The Analyst JO - Analyst VL - 136 IS - 4 N2 - A method combining immunomagnetic separation (IMS) and surface-enhanced Raman scattering (SERS) was developed to enumerate Escherichia coli (E. coli). Gold-coated magnetic spherical nanoparticles were prepared by immobilizing biotin-labeled anti-E. coli antibodies onto avidin-coated magnetic nanoparticles and used in the separation and concentration of the E. coli cells. Raman labels have been constructed using rod shaped gold nanoparticles coated with 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB) and subsequently with a molecular recognizer. Then DTNB-labeled gold nanorods were interacted with gold-coated magnetic spherical nanoparticle-antibody-E. coli complex. The capture efficiency and calibration graphs were obtained and examined in different E. coli concentrations (10(1)-10(7) cfu mL(-1)). The correlation between the concentration of bacteria and SERS signal was found to be linear within the range of 10(1)-10(4) cfu mL(-1) (R(2) = 0.992). The limit of detection (LOD) and limit of quantification (LOQ) values of the developed method were found to be 8 and 24 cfu mL(-1), respectively. The selectivity of the developed immunoassay was examined with Enterobacter aerogenes, Enterobacter dissolvens, and Salmonella enteriditis which did not produce any significant response. The ability of the immunoassay to detect E. coli in real water samples was also investigated and the results were compared with the experimental results from plate-counting methods. There was no significant difference between the methods that were compared (p > 0.05). This method is rapid and sensitive to target organisms with a total analysis time of less than 70 min. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/21125089/SERS_based_sandwich_immunoassay_using_antibody_coated_magnetic_nanoparticles_for_Escherichia_coli_enumeration_ L2 - https://doi.org/10.1039/c0an00473a DB - PRIME DP - Unbound Medicine ER -