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Serotonin receptor (5-HT 2A) and catechol-O-methyltransferase (COMT) gene polymorphisms: triggers of fibromyalgia?

Abstract

INTRODUCTION

Fibromyalgia is a rheumatic syndrome characterized by diffuse and chronic pain associated with fatigue, sleep disorders, anxiety, depression, memory loss, and dizziness. Although the physiological mechanisms that control fibromyalgia have not been precisely established, neuroendocrine, genetic or molecular factors may be involved in fibromyalgia.

OBJECTIVE

The aim of the present study was to characterize serotonin receptor (5-HT2A) and catechol-O-methyltransferase (COMT) gene polymorphisms in Brazilian patients with fibromyalgia and to evaluate the participation of these polymorphisms in the etiology of the disease.

MATERIAL AND METHODS

Genomic DNA extracted from 102 blood samples (51 patients, 51 controls) was used for molecular characterization of the 5-HT2A and COMT gene polymorphisms by PCR-RFLP.

RESULTS

Analysis of the 5-HT2A polymorphism revealed a frequency of 25.49% C/C, 49.02% T/C and 25.49% T/T in patients, and of 17.65% C/C, 62.74% T/C and 19.61% T/T in the control group, with no differences between the two groups.Analysis of the COMT polymorphism in patients showed a frequency of 17.65% and 45.10% for genotypes H/H and L/H, respectively. In the control group the frequency was 29.42% for H/H and 60.78% for L/H, also with no differences between the two groups. However, there was a significant difference in the frequency of the L/L genotype between patients (37.25%) and controls (9.8%), which permitted differentiation between the two groups.

CONCLUSION

The L/L genotype was more frequent among fibromyalgia patients. Though considering a polygenic situation and environmental factors, the molecular study of the rs4680 SNP of the COMT gene may be helpful to the identification of susceptible individuals.

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  • Authors+Show Affiliations

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    Unidade de Biotecnologia, Universidade de Ribeirão Preto, SP, Brasil.

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    Source

    MeSH

    Catechol O-Methyltransferase
    Female
    Fibromyalgia
    Humans
    Middle Aged
    Polymorphism, Genetic
    Receptor, Serotonin, 5-HT2A
    Surveys and Questionnaires

    Pub Type(s)

    Journal Article

    Language

    eng por

    PubMed ID

    21125150

    Citation

    Matsuda, Josie Budag, et al. "Serotonin Receptor (5-HT 2A) and catechol-O-methyltransferase (COMT) Gene Polymorphisms: Triggers of Fibromyalgia?" Revista Brasileira De Reumatologia, vol. 50, no. 2, 2010, pp. 141-9.
    Matsuda JB, Barbosa FR, Morel LJ, et al. Serotonin receptor (5-HT 2A) and catechol-O-methyltransferase (COMT) gene polymorphisms: triggers of fibromyalgia? Rev Bras Reumatol. 2010;50(2):141-9.
    Matsuda, J. B., Barbosa, F. R., Morel, L. J., França, S. d. e. . C., Zingaretti, S. M., da Silva, L. M., ... Fachin, A. L. (2010). Serotonin receptor (5-HT 2A) and catechol-O-methyltransferase (COMT) gene polymorphisms: triggers of fibromyalgia? Revista Brasileira De Reumatologia, 50(2), pp. 141-9.
    Matsuda JB, et al. Serotonin Receptor (5-HT 2A) and catechol-O-methyltransferase (COMT) Gene Polymorphisms: Triggers of Fibromyalgia. Rev Bras Reumatol. 2010;50(2):141-9. PubMed PMID: 21125150.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Serotonin receptor (5-HT 2A) and catechol-O-methyltransferase (COMT) gene polymorphisms: triggers of fibromyalgia? AU - Matsuda,Josie Budag, AU - Barbosa,Flávia Regina, AU - Morel,Lucas Junqueira Fernandes, AU - França,Suzelei de Castro, AU - Zingaretti,Sonia Marli, AU - da Silva,Lucienir Maria, AU - Pereira,Ana Maria Soares, AU - Marins,Mozart, AU - Fachin,Ana Lúcia, PY - 2009/05/07/received PY - 2010/02/26/accepted PY - 2010/12/3/entrez PY - 2010/12/3/pubmed PY - 2011/6/17/medline SP - 141 EP - 9 JF - Revista brasileira de reumatologia JO - Rev Bras Reumatol VL - 50 IS - 2 N2 - INTRODUCTION: Fibromyalgia is a rheumatic syndrome characterized by diffuse and chronic pain associated with fatigue, sleep disorders, anxiety, depression, memory loss, and dizziness. Although the physiological mechanisms that control fibromyalgia have not been precisely established, neuroendocrine, genetic or molecular factors may be involved in fibromyalgia. OBJECTIVE: The aim of the present study was to characterize serotonin receptor (5-HT2A) and catechol-O-methyltransferase (COMT) gene polymorphisms in Brazilian patients with fibromyalgia and to evaluate the participation of these polymorphisms in the etiology of the disease. MATERIAL AND METHODS: Genomic DNA extracted from 102 blood samples (51 patients, 51 controls) was used for molecular characterization of the 5-HT2A and COMT gene polymorphisms by PCR-RFLP. RESULTS: Analysis of the 5-HT2A polymorphism revealed a frequency of 25.49% C/C, 49.02% T/C and 25.49% T/T in patients, and of 17.65% C/C, 62.74% T/C and 19.61% T/T in the control group, with no differences between the two groups.Analysis of the COMT polymorphism in patients showed a frequency of 17.65% and 45.10% for genotypes H/H and L/H, respectively. In the control group the frequency was 29.42% for H/H and 60.78% for L/H, also with no differences between the two groups. However, there was a significant difference in the frequency of the L/L genotype between patients (37.25%) and controls (9.8%), which permitted differentiation between the two groups. CONCLUSION: The L/L genotype was more frequent among fibromyalgia patients. Though considering a polygenic situation and environmental factors, the molecular study of the rs4680 SNP of the COMT gene may be helpful to the identification of susceptible individuals. SN - 1809-4570 UR - https://www.unboundmedicine.com/medline/citation/21125150/Serotonin_receptor__5_HT_2A__and_catechol_O_methyltransferase__COMT__gene_polymorphisms:_triggers_of_fibromyalgia L2 - http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0482-50042010000200004&lng=en&nrm=iso&tlng=en DB - PRIME DP - Unbound Medicine ER -