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Comparison of the temporal programs regulating tyrosine hydroxylase and enkephalin expressions in TIDA neurons of lactating rats following pup removal and then pup return.
J Mol Neurosci. 2011 Oct; 45(2):110-8.JM

Abstract

Dopamine (DA) and enkephalin (ENK) release from the tuberoinfundibular dopaminergic neurons (TIDA) into the hypophysial portal circulation is fundamentally different under non-lactating and lactating conditions. The aim of this experiment was to compare the effect of a brief interruption then resumption of suckling on the temporal program of tyrosine hydroxylase (TH; rate-limiting enzyme of dopamine synthesis) and ENK regulation in dams. On post partum day 10, pups were removed for a 4-h period from a group of the dams then returned for 4- and 24-h periods. It was examined whether such a brief interruption of suckling provokes full up-regulation of TH and down-regulation of ENK, and whether reinitiation of suckling limits the extent to which TH up- and ENK down-regulate. At the end of experiment, the animals were decapitated. In situ hybridization was used to examine the expression of TH and ENK mRNA in the arcuate nucleus where TIDA neurons reside. The results showed that, on one hand, the removal of pups induced TH up-regulation, on the other hand, ENK expression also increased 8 h after removal of pups and then started to slowly decline. In dams whose sucklings were reinitiated both TH and ENK mRNAs were up-regulated at least for a day. ENK expression responded more slowly to the removal of pups than expression of TH, and after reinitiation of suckling, the temporal program of regulation of both TH and ENK expressions ran parallel in the first 24 h.

Authors+Show Affiliations

White House Clinics, 401 Highland Park Drive, Richmond, KY 40475, USA. fkszabo@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

21125428

Citation

Szabó, Flora Klara, et al. "Comparison of the Temporal Programs Regulating Tyrosine Hydroxylase and Enkephalin Expressions in TIDA Neurons of Lactating Rats Following Pup Removal and Then Pup Return." Journal of Molecular Neuroscience : MN, vol. 45, no. 2, 2011, pp. 110-8.
Szabó FK, Le WW, Snyder NS, et al. Comparison of the temporal programs regulating tyrosine hydroxylase and enkephalin expressions in TIDA neurons of lactating rats following pup removal and then pup return. J Mol Neurosci. 2011;45(2):110-8.
Szabó, F. K., Le, W. W., Snyder, N. S., & Hoffman, G. E. (2011). Comparison of the temporal programs regulating tyrosine hydroxylase and enkephalin expressions in TIDA neurons of lactating rats following pup removal and then pup return. Journal of Molecular Neuroscience : MN, 45(2), 110-8. https://doi.org/10.1007/s12031-010-9466-2
Szabó FK, et al. Comparison of the Temporal Programs Regulating Tyrosine Hydroxylase and Enkephalin Expressions in TIDA Neurons of Lactating Rats Following Pup Removal and Then Pup Return. J Mol Neurosci. 2011;45(2):110-8. PubMed PMID: 21125428.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of the temporal programs regulating tyrosine hydroxylase and enkephalin expressions in TIDA neurons of lactating rats following pup removal and then pup return. AU - Szabó,Flora Klara, AU - Le,Wei-Wei, AU - Snyder,Natalie S, AU - Hoffman,Gloria E, Y1 - 2010/12/02/ PY - 2010/09/07/received PY - 2010/10/19/accepted PY - 2010/12/3/entrez PY - 2010/12/3/pubmed PY - 2012/4/25/medline SP - 110 EP - 8 JF - Journal of molecular neuroscience : MN JO - J Mol Neurosci VL - 45 IS - 2 N2 - Dopamine (DA) and enkephalin (ENK) release from the tuberoinfundibular dopaminergic neurons (TIDA) into the hypophysial portal circulation is fundamentally different under non-lactating and lactating conditions. The aim of this experiment was to compare the effect of a brief interruption then resumption of suckling on the temporal program of tyrosine hydroxylase (TH; rate-limiting enzyme of dopamine synthesis) and ENK regulation in dams. On post partum day 10, pups were removed for a 4-h period from a group of the dams then returned for 4- and 24-h periods. It was examined whether such a brief interruption of suckling provokes full up-regulation of TH and down-regulation of ENK, and whether reinitiation of suckling limits the extent to which TH up- and ENK down-regulate. At the end of experiment, the animals were decapitated. In situ hybridization was used to examine the expression of TH and ENK mRNA in the arcuate nucleus where TIDA neurons reside. The results showed that, on one hand, the removal of pups induced TH up-regulation, on the other hand, ENK expression also increased 8 h after removal of pups and then started to slowly decline. In dams whose sucklings were reinitiated both TH and ENK mRNAs were up-regulated at least for a day. ENK expression responded more slowly to the removal of pups than expression of TH, and after reinitiation of suckling, the temporal program of regulation of both TH and ENK expressions ran parallel in the first 24 h. SN - 1559-1166 UR - https://www.unboundmedicine.com/medline/citation/21125428/Comparison_of_the_temporal_programs_regulating_tyrosine_hydroxylase_and_enkephalin_expressions_in_TIDA_neurons_of_lactating_rats_following_pup_removal_and_then_pup_return_ L2 - https://dx.doi.org/10.1007/s12031-010-9466-2 DB - PRIME DP - Unbound Medicine ER -