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Physiological, genetic, and molecular characterization of a high-Cd-accumulating rice cultivar, Jarjan.
J Exp Bot. 2011 Apr; 62(7):2265-72.JE

Abstract

Cadmium (Cd) in rice is a major source of Cd intake for people on a staple rice diet. The mechanisms underlying Cd accumulation in rice plant are still poorly understood. Here, we characterized the physiology and genetics of Cd transport in a high-Cd-accumulating cultivar (Jarjan) of rice (Oryza sativa). Jarjan showed 5- to 34-fold higher Cd accumulation in the shoots and grains than the cultivar Nipponbare, when it was grown in either a non-Cd-contaminated or a Cd-contaminated soil. A short-term uptake experiment showed no significant difference in Cd uptake by the roots between the two cultivars. However, Jarjan translocated 49% of the total Cd taken up to the shoots, whereas Nipponbare retained most of the Cd in the roots. In both concentration- and time-dependent experiments, Jarjan showed a superior capacity for root-to-shoot translocation of Cd. These results indicate that the high-Cd-accumulation phenotype in Jarjan results from efficient translocation of Cd from roots to shoots. Genetic analysis using an F(2) population derived from Jarjan and Nipponbare revealed that plants showing high- and low-Cd-accumulation phenotypes segregated in a 1:3 ratio, indicating that high accumulation in Jarjan is controlled by a single recessive gene. Furthermore, we isolated OsHMA3, a gene encoding a tonoplast-localized Cd transporter from Jarjan. The OsHMA3 protein was localized in all roots cells, but the sequence has a mutation leading to loss of function. Therefore, failure to sequester Cd into the root vacuoles by OsHMA3 is probably responsible for high Cd accumulation in Jarjan.

Authors+Show Affiliations

Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21127026

Citation

Ueno, Daisei, et al. "Physiological, Genetic, and Molecular Characterization of a high-Cd-accumulating Rice Cultivar, Jarjan." Journal of Experimental Botany, vol. 62, no. 7, 2011, pp. 2265-72.
Ueno D, Koyama E, Yamaji N, et al. Physiological, genetic, and molecular characterization of a high-Cd-accumulating rice cultivar, Jarjan. J Exp Bot. 2011;62(7):2265-72.
Ueno, D., Koyama, E., Yamaji, N., & Ma, J. F. (2011). Physiological, genetic, and molecular characterization of a high-Cd-accumulating rice cultivar, Jarjan. Journal of Experimental Botany, 62(7), 2265-72. https://doi.org/10.1093/jxb/erq383
Ueno D, et al. Physiological, Genetic, and Molecular Characterization of a high-Cd-accumulating Rice Cultivar, Jarjan. J Exp Bot. 2011;62(7):2265-72. PubMed PMID: 21127026.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Physiological, genetic, and molecular characterization of a high-Cd-accumulating rice cultivar, Jarjan. AU - Ueno,Daisei, AU - Koyama,Emi, AU - Yamaji,Naoki, AU - Ma,Jian Feng, Y1 - 2010/12/02/ PY - 2010/12/4/entrez PY - 2010/12/4/pubmed PY - 2011/8/9/medline SP - 2265 EP - 72 JF - Journal of experimental botany JO - J Exp Bot VL - 62 IS - 7 N2 - Cadmium (Cd) in rice is a major source of Cd intake for people on a staple rice diet. The mechanisms underlying Cd accumulation in rice plant are still poorly understood. Here, we characterized the physiology and genetics of Cd transport in a high-Cd-accumulating cultivar (Jarjan) of rice (Oryza sativa). Jarjan showed 5- to 34-fold higher Cd accumulation in the shoots and grains than the cultivar Nipponbare, when it was grown in either a non-Cd-contaminated or a Cd-contaminated soil. A short-term uptake experiment showed no significant difference in Cd uptake by the roots between the two cultivars. However, Jarjan translocated 49% of the total Cd taken up to the shoots, whereas Nipponbare retained most of the Cd in the roots. In both concentration- and time-dependent experiments, Jarjan showed a superior capacity for root-to-shoot translocation of Cd. These results indicate that the high-Cd-accumulation phenotype in Jarjan results from efficient translocation of Cd from roots to shoots. Genetic analysis using an F(2) population derived from Jarjan and Nipponbare revealed that plants showing high- and low-Cd-accumulation phenotypes segregated in a 1:3 ratio, indicating that high accumulation in Jarjan is controlled by a single recessive gene. Furthermore, we isolated OsHMA3, a gene encoding a tonoplast-localized Cd transporter from Jarjan. The OsHMA3 protein was localized in all roots cells, but the sequence has a mutation leading to loss of function. Therefore, failure to sequester Cd into the root vacuoles by OsHMA3 is probably responsible for high Cd accumulation in Jarjan. SN - 1460-2431 UR - https://www.unboundmedicine.com/medline/citation/21127026/Physiological_genetic_and_molecular_characterization_of_a_high_Cd_accumulating_rice_cultivar_Jarjan_ DB - PRIME DP - Unbound Medicine ER -