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SIRT1 deficiency attenuates MPP+-induced apoptosis in dopaminergic cells.
FEBS Lett. 2011 Jan 03; 585(1):219-24.FL

Abstract

One of the functions mediated by sirtuin 1 (SIRT1), the NAD(+)-dependent protein deacetylase, has been suggested to be neuroprotective since resveratrol, a SIRT1 activator, inhibits 1-methyl-4-phenylpyridinium ion (MPP(+))-induced cytotoxicity. In this study, we show that SIRT1 siRNA transfection blocks MPP(+)-induced apoptosis in SH-SY5Y cells. The ratio of potential pro-apoptotic BNIP2 to antiapoptotic BCL-xL was attenuated in SIRT1-deficient cells following MPP(+) treatment. In addition, BNIP2 shRNA-transfected cells showed reduced cleavage of PARP-1, while BNIP2 overexpression intensified the cleavage in MPP(+)-treated SH-SY5Y cells, suggesting that BNIP2 participates in the MPP(+)-induced apoptosis. Overall, these data imply that SIRT1 may mediate MPP(+)-induced cytotoxicity, possibly through the regulation of BNIP2.

Authors+Show Affiliations

Department of Pharmacology, School of Medicine, Kyung Hee University, Seoul, Republic of Korea.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21130087

Citation

Park, Geunyeong, et al. "SIRT1 Deficiency Attenuates MPP+-induced Apoptosis in Dopaminergic Cells." FEBS Letters, vol. 585, no. 1, 2011, pp. 219-24.
Park G, Jeong JW, Kim JE. SIRT1 deficiency attenuates MPP+-induced apoptosis in dopaminergic cells. FEBS Lett. 2011;585(1):219-24.
Park, G., Jeong, J. W., & Kim, J. E. (2011). SIRT1 deficiency attenuates MPP+-induced apoptosis in dopaminergic cells. FEBS Letters, 585(1), 219-24. https://doi.org/10.1016/j.febslet.2010.11.048
Park G, Jeong JW, Kim JE. SIRT1 Deficiency Attenuates MPP+-induced Apoptosis in Dopaminergic Cells. FEBS Lett. 2011 Jan 3;585(1):219-24. PubMed PMID: 21130087.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SIRT1 deficiency attenuates MPP+-induced apoptosis in dopaminergic cells. AU - Park,Geunyeong, AU - Jeong,Joo-Won, AU - Kim,Ja-Eun, Y1 - 2010/12/02/ PY - 2010/09/13/received PY - 2010/11/13/revised PY - 2010/11/25/accepted PY - 2010/12/7/entrez PY - 2010/12/7/pubmed PY - 2011/3/16/medline SP - 219 EP - 24 JF - FEBS letters JO - FEBS Lett VL - 585 IS - 1 N2 - One of the functions mediated by sirtuin 1 (SIRT1), the NAD(+)-dependent protein deacetylase, has been suggested to be neuroprotective since resveratrol, a SIRT1 activator, inhibits 1-methyl-4-phenylpyridinium ion (MPP(+))-induced cytotoxicity. In this study, we show that SIRT1 siRNA transfection blocks MPP(+)-induced apoptosis in SH-SY5Y cells. The ratio of potential pro-apoptotic BNIP2 to antiapoptotic BCL-xL was attenuated in SIRT1-deficient cells following MPP(+) treatment. In addition, BNIP2 shRNA-transfected cells showed reduced cleavage of PARP-1, while BNIP2 overexpression intensified the cleavage in MPP(+)-treated SH-SY5Y cells, suggesting that BNIP2 participates in the MPP(+)-induced apoptosis. Overall, these data imply that SIRT1 may mediate MPP(+)-induced cytotoxicity, possibly through the regulation of BNIP2. SN - 1873-3468 UR - https://www.unboundmedicine.com/medline/citation/21130087/SIRT1_deficiency_attenuates_MPP+_induced_apoptosis_in_dopaminergic_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-5793(10)00966-X DB - PRIME DP - Unbound Medicine ER -