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Molecular cloning and ontogenic mRNA expression of fatty acid desaturase in the carnivorous striped snakehead fish (Channa striata).

Abstract

There is very little information on the capacity of freshwater carnivorous fish to biosynthesize highly unsaturated fatty acids (HUFA). The striped snakehead fish (Channa striata) is a carnivorous species cultured inland of several Southeast Asian countries due to its pharmaceutical properties in wound healing enhancement. We described here the full-length cDNA cloning of a striped snakehead fatty acid desaturase (fads), which is responsible for desaturation of unsaturated fatty acids in the HUFA biosynthesis. Bioinformatics analysis reveals a protein coding region with length of 445 amino acids containing all characteristic features of desaturase enzyme, including a cytochrome b5-domain with the heme-binding motif, two transmembrane domains and three histidine-rich regions. The striped snakehead fads amino acid sequence shares high similarity with known fads of other teleosts. The mRNA expression of striped snakehead fads also showed an ontogenic-related increase in expression in 0-20 days after hatch larva. Using ISH, we localized the presence of fads in larva brain, liver and intestinal tissues.

Authors+Show Affiliations

School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21130179

Citation

Jaya-Ram, Annette, et al. "Molecular Cloning and Ontogenic mRNA Expression of Fatty Acid Desaturase in the Carnivorous Striped Snakehead Fish (Channa Striata)." Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, vol. 158, no. 4, 2011, pp. 415-22.
Jaya-Ram A, Ishak SD, Enyu YL, et al. Molecular cloning and ontogenic mRNA expression of fatty acid desaturase in the carnivorous striped snakehead fish (Channa striata). Comp Biochem Physiol, Part A Mol Integr Physiol. 2011;158(4):415-22.
Jaya-Ram, A., Ishak, S. D., Enyu, Y. L., Kuah, M. K., Wong, K. L., & Shu-Chien, A. C. (2011). Molecular cloning and ontogenic mRNA expression of fatty acid desaturase in the carnivorous striped snakehead fish (Channa striata). Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, 158(4), pp. 415-22. doi:10.1016/j.cbpa.2010.11.018.
Jaya-Ram A, et al. Molecular Cloning and Ontogenic mRNA Expression of Fatty Acid Desaturase in the Carnivorous Striped Snakehead Fish (Channa Striata). Comp Biochem Physiol, Part A Mol Integr Physiol. 2011;158(4):415-22. PubMed PMID: 21130179.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular cloning and ontogenic mRNA expression of fatty acid desaturase in the carnivorous striped snakehead fish (Channa striata). AU - Jaya-Ram,Annette, AU - Ishak,Sairatul Dahlianis, AU - Enyu,Yee-Ling, AU - Kuah,Meng-Kiat, AU - Wong,Kah-Loon, AU - Shu-Chien,Alexander Chong, Y1 - 2010/12/01/ PY - 2010/08/09/received PY - 2010/11/24/revised PY - 2010/11/24/accepted PY - 2010/12/7/entrez PY - 2010/12/7/pubmed PY - 2011/8/16/medline SP - 415 EP - 22 JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology JO - Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. VL - 158 IS - 4 N2 - There is very little information on the capacity of freshwater carnivorous fish to biosynthesize highly unsaturated fatty acids (HUFA). The striped snakehead fish (Channa striata) is a carnivorous species cultured inland of several Southeast Asian countries due to its pharmaceutical properties in wound healing enhancement. We described here the full-length cDNA cloning of a striped snakehead fatty acid desaturase (fads), which is responsible for desaturation of unsaturated fatty acids in the HUFA biosynthesis. Bioinformatics analysis reveals a protein coding region with length of 445 amino acids containing all characteristic features of desaturase enzyme, including a cytochrome b5-domain with the heme-binding motif, two transmembrane domains and three histidine-rich regions. The striped snakehead fads amino acid sequence shares high similarity with known fads of other teleosts. The mRNA expression of striped snakehead fads also showed an ontogenic-related increase in expression in 0-20 days after hatch larva. Using ISH, we localized the presence of fads in larva brain, liver and intestinal tissues. SN - 1531-4332 UR - https://www.unboundmedicine.com/medline/citation/21130179/Molecular_cloning_and_ontogenic_mRNA_expression_of_fatty_acid_desaturase_in_the_carnivorous_striped_snakehead_fish__Channa_striata__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1095-6433(10)00541-6 DB - PRIME DP - Unbound Medicine ER -