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ST11, the dominant clone of KPC-producing Klebsiella pneumoniae in China.
J Antimicrob Chemother. 2011 Feb; 66(2):307-12.JA

Abstract

OBJECTIVES

Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae has spread rapidly in China. In this study, we aimed to investigate the molecular epidemiology of KPC-producing K. pneumoniae isolates in China.

METHODS

Ninety-five carbapenem-resistant K. pneumoniae isolates from 13 hospitals in nine cities covering five provinces in China were analysed. Antibiotic susceptibility was determined by the Etest. Multilocus sequence typing (MLST) and PFGE were used for epidemiological analysis. The genetic structure around bla(KPC) and the encoding genes of extended-spectrum β-lactamases and plasmid-mediated AmpC enzymes were detected by PCR and sequencing. Plasmids were analysed by transformation, restriction and Southern blot.

RESULTS

All isolates harboured the bla(KPC-2) gene. Seven sequence types (STs) were obtained. The dominant clone was ST11 (61/95), which was identified in isolates from Zhejiang province (four hospitals in Hangzhou and one hospital in Ningbo), Jiangsu province (one hospital in Nanjing) and Anhui province (one hospital in Hefei). Isolates with ST11 showed >80% similarity in PFGE patterns. Plasmids from 14 selected transformants, their original isolates representing different STs and/or regions, had a diversity of HindIII restriction maps.

CONCLUSIONS

The dominant clone of KPC-producing K. pneumoniae in China is ST11, which is closely related to ST258, which has been reported worldwide.

Authors+Show Affiliations

Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21131324

Citation

Qi, Yan, et al. "ST11, the Dominant Clone of KPC-producing Klebsiella Pneumoniae in China." The Journal of Antimicrobial Chemotherapy, vol. 66, no. 2, 2011, pp. 307-12.
Qi Y, Wei Z, Ji S, et al. ST11, the dominant clone of KPC-producing Klebsiella pneumoniae in China. J Antimicrob Chemother. 2011;66(2):307-12.
Qi, Y., Wei, Z., Ji, S., Du, X., Shen, P., & Yu, Y. (2011). ST11, the dominant clone of KPC-producing Klebsiella pneumoniae in China. The Journal of Antimicrobial Chemotherapy, 66(2), 307-12. https://doi.org/10.1093/jac/dkq431
Qi Y, et al. ST11, the Dominant Clone of KPC-producing Klebsiella Pneumoniae in China. J Antimicrob Chemother. 2011;66(2):307-12. PubMed PMID: 21131324.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ST11, the dominant clone of KPC-producing Klebsiella pneumoniae in China. AU - Qi,Yan, AU - Wei,Zeqing, AU - Ji,Shujuan, AU - Du,Xiaoxing, AU - Shen,Ping, AU - Yu,Yunsong, Y1 - 2010/12/03/ PY - 2010/12/7/entrez PY - 2010/12/7/pubmed PY - 2011/10/5/medline SP - 307 EP - 12 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 66 IS - 2 N2 - OBJECTIVES: Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae has spread rapidly in China. In this study, we aimed to investigate the molecular epidemiology of KPC-producing K. pneumoniae isolates in China. METHODS: Ninety-five carbapenem-resistant K. pneumoniae isolates from 13 hospitals in nine cities covering five provinces in China were analysed. Antibiotic susceptibility was determined by the Etest. Multilocus sequence typing (MLST) and PFGE were used for epidemiological analysis. The genetic structure around bla(KPC) and the encoding genes of extended-spectrum β-lactamases and plasmid-mediated AmpC enzymes were detected by PCR and sequencing. Plasmids were analysed by transformation, restriction and Southern blot. RESULTS: All isolates harboured the bla(KPC-2) gene. Seven sequence types (STs) were obtained. The dominant clone was ST11 (61/95), which was identified in isolates from Zhejiang province (four hospitals in Hangzhou and one hospital in Ningbo), Jiangsu province (one hospital in Nanjing) and Anhui province (one hospital in Hefei). Isolates with ST11 showed >80% similarity in PFGE patterns. Plasmids from 14 selected transformants, their original isolates representing different STs and/or regions, had a diversity of HindIII restriction maps. CONCLUSIONS: The dominant clone of KPC-producing K. pneumoniae in China is ST11, which is closely related to ST258, which has been reported worldwide. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/21131324/ST11_the_dominant_clone_of_KPC_producing_Klebsiella_pneumoniae_in_China_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkq431 DB - PRIME DP - Unbound Medicine ER -