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Diet fat composition alters membrane phospholipid composition, insulin binding, and glucose metabolism in adipocytes from control and diabetic animals.
J Biol Chem. 1990 Jul 05; 265(19):11143-50.JB

Abstract

The present study was designed to determine if diet fat-induced alteration in the fatty acid composition of the adipocyte plasma membrane alters insulin binding and the insulin responsiveness of glucose metabolism in control and diabetic states. Normal (control) and diabetic (streptozotocin-induced) rats were fed high fat semipurified diets providing a high or low polyunsaturated to saturated fatty acid (P/S) ratio. Feeding a high P/S diet increased the polyunsaturated fatty acid content of major membrane phospholipids of the adipocyte plasma membrane from both normal and diabetic animals. The diabetic state was associated with an elevated content of linoleic acid and a reduced level of arachidonic acid consistent with reduced delta 6-desaturation. Feeding the high P/S diet to diabetic animals increased membrane linoleic acid content and prevented the decrease observed in the arachidonic acid of membrane phospholipids. The high P/S diet was associated with increased insulin binding in nondiabetic animals but did not change the amount of insulin bound by cells from diabetic animals. Significantly (p less than 0.05) increased rates of insulin-stimulated glucose transport and lipogenesis (glucose incorporation into lipids) were observed in control animals fed the high as compared to the low P/S diet. The rates of insulin-stimulated glucose transport, oxidation, and lipogenesis were lower (p less than 0.05) for cells from diabetic as compared to control animals. However, feeding a high P/S diet significantly improved rates for all three of these functions (p less than 0.05). It is concluded that diet-induced alterations in membrane composition may provide a mechanism for improving the cellular response to insulin in cells from diabetic animals.

Authors+Show Affiliations

Nutrition and Metabolism Research Group, Faculty of Medicine, University of Alberta, Edmonton, Canada.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2113530

Citation

Field, C J., et al. "Diet Fat Composition Alters Membrane Phospholipid Composition, Insulin Binding, and Glucose Metabolism in Adipocytes From Control and Diabetic Animals." The Journal of Biological Chemistry, vol. 265, no. 19, 1990, pp. 11143-50.
Field CJ, Ryan EA, Thomson AB, et al. Diet fat composition alters membrane phospholipid composition, insulin binding, and glucose metabolism in adipocytes from control and diabetic animals. J Biol Chem. 1990;265(19):11143-50.
Field, C. J., Ryan, E. A., Thomson, A. B., & Clandinin, M. T. (1990). Diet fat composition alters membrane phospholipid composition, insulin binding, and glucose metabolism in adipocytes from control and diabetic animals. The Journal of Biological Chemistry, 265(19), 11143-50.
Field CJ, et al. Diet Fat Composition Alters Membrane Phospholipid Composition, Insulin Binding, and Glucose Metabolism in Adipocytes From Control and Diabetic Animals. J Biol Chem. 1990 Jul 5;265(19):11143-50. PubMed PMID: 2113530.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diet fat composition alters membrane phospholipid composition, insulin binding, and glucose metabolism in adipocytes from control and diabetic animals. AU - Field,C J, AU - Ryan,E A, AU - Thomson,A B, AU - Clandinin,M T, PY - 1990/7/5/pubmed PY - 1990/7/5/medline PY - 1990/7/5/entrez SP - 11143 EP - 50 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 265 IS - 19 N2 - The present study was designed to determine if diet fat-induced alteration in the fatty acid composition of the adipocyte plasma membrane alters insulin binding and the insulin responsiveness of glucose metabolism in control and diabetic states. Normal (control) and diabetic (streptozotocin-induced) rats were fed high fat semipurified diets providing a high or low polyunsaturated to saturated fatty acid (P/S) ratio. Feeding a high P/S diet increased the polyunsaturated fatty acid content of major membrane phospholipids of the adipocyte plasma membrane from both normal and diabetic animals. The diabetic state was associated with an elevated content of linoleic acid and a reduced level of arachidonic acid consistent with reduced delta 6-desaturation. Feeding the high P/S diet to diabetic animals increased membrane linoleic acid content and prevented the decrease observed in the arachidonic acid of membrane phospholipids. The high P/S diet was associated with increased insulin binding in nondiabetic animals but did not change the amount of insulin bound by cells from diabetic animals. Significantly (p less than 0.05) increased rates of insulin-stimulated glucose transport and lipogenesis (glucose incorporation into lipids) were observed in control animals fed the high as compared to the low P/S diet. The rates of insulin-stimulated glucose transport, oxidation, and lipogenesis were lower (p less than 0.05) for cells from diabetic as compared to control animals. However, feeding a high P/S diet significantly improved rates for all three of these functions (p less than 0.05). It is concluded that diet-induced alterations in membrane composition may provide a mechanism for improving the cellular response to insulin in cells from diabetic animals. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/2113530/Diet_fat_composition_alters_membrane_phospholipid_composition_insulin_binding_and_glucose_metabolism_in_adipocytes_from_control_and_diabetic_animals_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=2113530 DB - PRIME DP - Unbound Medicine ER -