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Differential gene expression in Daphnia magna suggests distinct modes of action and bioavailability for ZnO nanoparticles and Zn ions.
Environ Sci Technol. 2011 Jan 15; 45(2):762-8.ES

Abstract

Zinc oxide nanoparticles (ZnO NPs) are being rapidly developed for use in consumer products, wastewater treatment, and chemotherapy providing several possible routes for ZnO NP exposure to humans and aquatic organisms. Recent studies have shown that ZnO NPs undergo rapid dissolution to Zn(2+), but the relative contribution of Zn(2+) to ZnO NP bioavailability and toxicity is not clear. We show that a fraction of the ZnO NPs in suspension dissolves, and this fraction cannot account for the toxicity of the ZnO NP suspensions to Daphnia magna. Gene expression profiling of D. magna exposed to ZnO NPs or ZnSO(4) at sublethal concentrations revealed distinct modes of toxicity. There was also little overlap in gene expression between ZnO NPs and SiO(x) NPs, suggesting specificity for the ZnO NP expression profile. ZnO NPs effected expression of genes involved in cytoskeletal transport, cellular respiration, and reproduction. A specific pattern of differential expression of three biomarker genes including a multicystatin, ferritin, and C1q containing gene were confirmed for ZnO NP exposure and provide a suite of biomarkers for identifying environmental exposure to ZnO NPs and differentiating between NP and ionic exposure.

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Authors+Show Affiliations

Poynton HC
National Exposure Research Laboratory, US Environmental Protection Agency, Cincinnati, Ohio, USA. helen.poynton@umb.edu
Lazorchak JM
No affiliation info available
Impellitteri CA
No affiliation info available
Smith ME
No affiliation info available
Rogers K
No affiliation info available
Patra M
No affiliation info available
Hammer KA
No affiliation info available
Allen HJ
No affiliation info available
Vulpe CD
No affiliation info available

MeSH

AnimalsBiomarkersCationsCell RespirationDaphniaGene ExpressionGene Expression ProfilingMetal NanoparticlesMolecular Sequence DataOxidative StressReproductionWater Pollutants, ChemicalZinc Oxide

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

21142172

Citation

Poynton, Helen C., et al. "Differential Gene Expression in Daphnia Magna Suggests Distinct Modes of Action and Bioavailability for ZnO Nanoparticles and Zn Ions." Environmental Science & Technology, vol. 45, no. 2, 2011, pp. 762-8.
Poynton HC, Lazorchak JM, Impellitteri CA, et al. Differential gene expression in Daphnia magna suggests distinct modes of action and bioavailability for ZnO nanoparticles and Zn ions. Environ Sci Technol. 2011;45(2):762-8.
Poynton, H. C., Lazorchak, J. M., Impellitteri, C. A., Smith, M. E., Rogers, K., Patra, M., Hammer, K. A., Allen, H. J., & Vulpe, C. D. (2011). Differential gene expression in Daphnia magna suggests distinct modes of action and bioavailability for ZnO nanoparticles and Zn ions. Environmental Science & Technology, 45(2), 762-8. https://doi.org/10.1021/es102501z
Poynton HC, et al. Differential Gene Expression in Daphnia Magna Suggests Distinct Modes of Action and Bioavailability for ZnO Nanoparticles and Zn Ions. Environ Sci Technol. 2011 Jan 15;45(2):762-8. PubMed PMID: 21142172.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential gene expression in Daphnia magna suggests distinct modes of action and bioavailability for ZnO nanoparticles and Zn ions. AU - Poynton,Helen C, AU - Lazorchak,James M, AU - Impellitteri,Christopher A, AU - Smith,Mark E, AU - Rogers,Kim, AU - Patra,Manomita, AU - Hammer,Katherine A, AU - Allen,H Joel, AU - Vulpe,Chris D, Y1 - 2010/12/13/ PY - 2010/12/15/entrez PY - 2010/12/15/pubmed PY - 2011/3/30/medline SP - 762 EP - 8 JF - Environmental science & technology JO - Environ Sci Technol VL - 45 IS - 2 N2 - Zinc oxide nanoparticles (ZnO NPs) are being rapidly developed for use in consumer products, wastewater treatment, and chemotherapy providing several possible routes for ZnO NP exposure to humans and aquatic organisms. Recent studies have shown that ZnO NPs undergo rapid dissolution to Zn(2+), but the relative contribution of Zn(2+) to ZnO NP bioavailability and toxicity is not clear. We show that a fraction of the ZnO NPs in suspension dissolves, and this fraction cannot account for the toxicity of the ZnO NP suspensions to Daphnia magna. Gene expression profiling of D. magna exposed to ZnO NPs or ZnSO(4) at sublethal concentrations revealed distinct modes of toxicity. There was also little overlap in gene expression between ZnO NPs and SiO(x) NPs, suggesting specificity for the ZnO NP expression profile. ZnO NPs effected expression of genes involved in cytoskeletal transport, cellular respiration, and reproduction. A specific pattern of differential expression of three biomarker genes including a multicystatin, ferritin, and C1q containing gene were confirmed for ZnO NP exposure and provide a suite of biomarkers for identifying environmental exposure to ZnO NPs and differentiating between NP and ionic exposure. SN - 1520-5851 UR - https://www.unboundmedicine.com/medline/citation/21142172/Differential_gene_expression_in_Daphnia_magna_suggests_distinct_modes_of_action_and_bioavailability_for_ZnO_nanoparticles_and_Zn_ions_ L2 - https://doi.org/10.1021/es102501z DB - PRIME DP - Unbound Medicine ER -