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Bioactivity of American ginseng by knockout extract preparation using monoclonal antibody.
Curr Drug Discov Technol. 2011 Mar; 8(1):32-41.CD

Abstract

We produced anti-ginsenoside Re (GRe)-4G10 monoclonal antibody that specifically recognizes GRe and used this to prepare an immunoaffinity column to remove GRe from American ginseng berry extract (AGBE) (thus producing GRe knock-out AGBE (GRe-KO-AGBE)). We compared the anti-diabetic and anti-obesity effects of AGBE and GRe-KO-AGBE in adult diabetic C57BL/6J ob/ob mice. Fasting blood glucose levels and body weight were measured in mice receiving daily intraperitoneal injections of 150 mg/kg of either extract for 27 consecutive days. On Day 6, the group taking AGBE had significantly lower fasting blood glucose levels compared to GRe-KO-AGBE-treated mice (70.5 mg/dl vs. 140 mg/dl). On Day 13, the glucose levels in both groups were 201 mg/dl for AGBE-treated group and 169 mg/dl for GRe-KO-AGBE, respectively. GRe (20 mg/kg) had dose-related effects on fasting blood glucose levels (reduced to 188 and 180 mg/dl on Day 5 and Day 12, respectively, compared to 229 and 235 mg/dl, respectively, in the vehicle group). This hypoglycemic effect persisted even after treatment discontinued (blood glucose levels at 3 days post-treatment: 198 and 253 mg/dl with ginsenoside and vehicle treatment, respectively). The monoclonal antibody technique can be used to prepare botanical knockout extracts for bioactivity studies.

Authors+Show Affiliations

Tang Center for Herbal Medicine Research, The Pritzker School of Medicine, The University of Chicago, 5841 S. Maryland Avenue, MC 4028, Chicago, IL 60637, USA. CYuan@airway2.bsd.uchicago.eduNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21143131

Citation

Yuan, Chun-Su, and Hiroyuki Tanaka. "Bioactivity of American Ginseng By Knockout Extract Preparation Using Monoclonal Antibody." Current Drug Discovery Technologies, vol. 8, no. 1, 2011, pp. 32-41.
Yuan CS, Tanaka H. Bioactivity of American ginseng by knockout extract preparation using monoclonal antibody. Curr Drug Discov Technol. 2011;8(1):32-41.
Yuan, C. S., & Tanaka, H. (2011). Bioactivity of American ginseng by knockout extract preparation using monoclonal antibody. Current Drug Discovery Technologies, 8(1), 32-41.
Yuan CS, Tanaka H. Bioactivity of American Ginseng By Knockout Extract Preparation Using Monoclonal Antibody. Curr Drug Discov Technol. 2011;8(1):32-41. PubMed PMID: 21143131.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bioactivity of American ginseng by knockout extract preparation using monoclonal antibody. AU - Yuan,Chun-Su, AU - Tanaka,Hiroyuki, PY - 2010/09/25/received PY - 2010/09/27/accepted PY - 2010/12/15/entrez PY - 2010/12/15/pubmed PY - 2011/9/29/medline SP - 32 EP - 41 JF - Current drug discovery technologies JO - Curr Drug Discov Technol VL - 8 IS - 1 N2 - We produced anti-ginsenoside Re (GRe)-4G10 monoclonal antibody that specifically recognizes GRe and used this to prepare an immunoaffinity column to remove GRe from American ginseng berry extract (AGBE) (thus producing GRe knock-out AGBE (GRe-KO-AGBE)). We compared the anti-diabetic and anti-obesity effects of AGBE and GRe-KO-AGBE in adult diabetic C57BL/6J ob/ob mice. Fasting blood glucose levels and body weight were measured in mice receiving daily intraperitoneal injections of 150 mg/kg of either extract for 27 consecutive days. On Day 6, the group taking AGBE had significantly lower fasting blood glucose levels compared to GRe-KO-AGBE-treated mice (70.5 mg/dl vs. 140 mg/dl). On Day 13, the glucose levels in both groups were 201 mg/dl for AGBE-treated group and 169 mg/dl for GRe-KO-AGBE, respectively. GRe (20 mg/kg) had dose-related effects on fasting blood glucose levels (reduced to 188 and 180 mg/dl on Day 5 and Day 12, respectively, compared to 229 and 235 mg/dl, respectively, in the vehicle group). This hypoglycemic effect persisted even after treatment discontinued (blood glucose levels at 3 days post-treatment: 198 and 253 mg/dl with ginsenoside and vehicle treatment, respectively). The monoclonal antibody technique can be used to prepare botanical knockout extracts for bioactivity studies. SN - 1875-6220 UR - https://www.unboundmedicine.com/medline/citation/21143131/Bioactivity_of_American_ginseng_by_knockout_extract_preparation_using_monoclonal_antibody_ L2 - http://www.eurekaselect.com/94342/article DB - PRIME DP - Unbound Medicine ER -