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Effects of T-2 toxin and selenium on chondrocyte expression of matrix metalloproteinases (MMP-1, MMP-13), α2-macroglobulin (α2M) and TIMPs.
Toxicol In Vitro. 2011 Mar; 25(2):492-9.TV

Abstract

T-2 toxin is regarded as an important etiological factor of Kashin-Beck disease, and supplementation of selenium-salt partly prevents Kashin-Beck disease. The present study investigated the effects of T-2 toxin on the degradation of type II collagen in human chondrocytes in vitro. Human chondrocytes were isolated and cultured on bone matrix gelatin to form an artificial cartilage model in vitro with or without T-2 toxin and selenium. Immunohistochemistry analyses showed that T-2 toxin decreased type II collagen staining and selenium appeared to prevent the decrease in type II collagen induced by T-2 toxin in engineered cartilage. Then, Western blot and RT-PCR analyses showed that an increase in MMP-13 and MMP-1 expressions, and a decrease in the expression of the general endoproteinase inhibitor (α(2)M) were induced by T-2 toxin. Gelatin reverse zymography showed that TIMP-1 and TIMP-2 levels were decreased in a dose-dependent manner after exposure of T-2 toxin. Selenium had a protective role by increasing the level of type II collagen protein through down-regulation of MMP-13 protein and mRNA expression and up-regulation of TIMP-1 and TIMP-2 expressions. These data suggest T-2 toxin induces cartilage matrix degradation by the up-regulation of MMP-13 and TIMP-1, and down-regulation of TIMP-2 and α(2)M expressions.

Authors+Show Affiliations

Institute of Endemic Diseases, Medical School of Xi'an Jiaotong University, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an, Shaanxi, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21144892

Citation

Chen, Jinghong, et al. "Effects of T-2 Toxin and Selenium On Chondrocyte Expression of Matrix Metalloproteinases (MMP-1, MMP-13), Α2-macroglobulin (α2M) and TIMPs." Toxicology in Vitro : an International Journal Published in Association With BIBRA, vol. 25, no. 2, 2011, pp. 492-9.
Chen J, Chu Y, Cao J, et al. Effects of T-2 toxin and selenium on chondrocyte expression of matrix metalloproteinases (MMP-1, MMP-13), α2-macroglobulin (α2M) and TIMPs. Toxicol In Vitro. 2011;25(2):492-9.
Chen, J., Chu, Y., Cao, J., Wang, W., Liu, J., & Wang, J. (2011). Effects of T-2 toxin and selenium on chondrocyte expression of matrix metalloproteinases (MMP-1, MMP-13), α2-macroglobulin (α2M) and TIMPs. Toxicology in Vitro : an International Journal Published in Association With BIBRA, 25(2), 492-9. https://doi.org/10.1016/j.tiv.2010.12.001
Chen J, et al. Effects of T-2 Toxin and Selenium On Chondrocyte Expression of Matrix Metalloproteinases (MMP-1, MMP-13), Α2-macroglobulin (α2M) and TIMPs. Toxicol In Vitro. 2011;25(2):492-9. PubMed PMID: 21144892.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of T-2 toxin and selenium on chondrocyte expression of matrix metalloproteinases (MMP-1, MMP-13), α2-macroglobulin (α2M) and TIMPs. AU - Chen,Jinghong, AU - Chu,Yonglie, AU - Cao,Junling, AU - Wang,Wei, AU - Liu,Jiayuan, AU - Wang,Jiali, Y1 - 2010/12/07/ PY - 2010/04/29/received PY - 2010/11/24/revised PY - 2010/12/01/accepted PY - 2010/12/15/entrez PY - 2010/12/15/pubmed PY - 2011/6/9/medline SP - 492 EP - 9 JF - Toxicology in vitro : an international journal published in association with BIBRA JO - Toxicol In Vitro VL - 25 IS - 2 N2 - T-2 toxin is regarded as an important etiological factor of Kashin-Beck disease, and supplementation of selenium-salt partly prevents Kashin-Beck disease. The present study investigated the effects of T-2 toxin on the degradation of type II collagen in human chondrocytes in vitro. Human chondrocytes were isolated and cultured on bone matrix gelatin to form an artificial cartilage model in vitro with or without T-2 toxin and selenium. Immunohistochemistry analyses showed that T-2 toxin decreased type II collagen staining and selenium appeared to prevent the decrease in type II collagen induced by T-2 toxin in engineered cartilage. Then, Western blot and RT-PCR analyses showed that an increase in MMP-13 and MMP-1 expressions, and a decrease in the expression of the general endoproteinase inhibitor (α(2)M) were induced by T-2 toxin. Gelatin reverse zymography showed that TIMP-1 and TIMP-2 levels were decreased in a dose-dependent manner after exposure of T-2 toxin. Selenium had a protective role by increasing the level of type II collagen protein through down-regulation of MMP-13 protein and mRNA expression and up-regulation of TIMP-1 and TIMP-2 expressions. These data suggest T-2 toxin induces cartilage matrix degradation by the up-regulation of MMP-13 and TIMP-1, and down-regulation of TIMP-2 and α(2)M expressions. SN - 1879-3177 UR - https://www.unboundmedicine.com/medline/citation/21144892/Effects_of_T_2_toxin_and_selenium_on_chondrocyte_expression_of_matrix_metalloproteinases__MMP_1_MMP_13__α2_macroglobulin__α2M__and_TIMPs_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0887-2333(10)00313-9 DB - PRIME DP - Unbound Medicine ER -