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Direct effects of iodothyronines on excess fat storage in rat hepatocytes.
J Hepatol. 2011 Jun; 54(6):1230-6.JH

Abstract

BACKGROUND & AIMS

Previous studies have demonstrated that 3,5-L-diiodothyronine (T(2)) is able to prevent lipid accumulation in the liver of rats fed a high-fat diet. Whether this effect is due to a direct action of T(2) on the liver has not been elucidated. In this study, we investigated the ability of T(2) to reduce the excess lipids in isolated hepatocytes treated with fatty acids (FFAs). The effects of T(2) were compared with those elicited by 3,3',5-L-triiodothyronine (T(3)).

METHODS

To mimic the fatty liver condition, primary cultures of rat hepatocytes were overloaded with lipids, by exposure to FFAs ("fatty hepatocytes"), and then treated with T(2) or T(3). Lipid content, morphometry of lipid droplets (LDs), and expression of the adipocyte differentiation-related protein (ADRP) and the peroxisome proliferator-activated receptors (PPAR-α, -γ, -δ) were evaluated. Activities of the lipolytic enzyme acyl CoA oxidase-AOX and the antioxidant enzymes superoxide dismutase-SOD and catalase-CAT were also determined.

RESULTS

FFA-induced lipid accumulation was associated with an increase in both number/size of LDs and expression of ADRP, PPAR-γ, and PPAR-δ/β mRNAs, as well as in the activities of AOX, SOD, and CAT. The addition of T(2) or T(3) to "fatty hepatocytes" resulted in a reduction in: (i) lipid content and LD diameter; (ii) PPAR-γ and PPAR-δ expression; (iii) activities of AOX and antioxidant enzymes.

CONCLUSIONS

These data demonstrate, for the first time, a direct action of both T(2) and T(3) in reducing the excess fat in cultured hepatocytes.

Authors+Show Affiliations

Dipartimento di Biologia, Università di Genova, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21145833

Citation

Grasselli, Elena, et al. "Direct Effects of Iodothyronines On Excess Fat Storage in Rat Hepatocytes." Journal of Hepatology, vol. 54, no. 6, 2011, pp. 1230-6.
Grasselli E, Voci A, Canesi L, et al. Direct effects of iodothyronines on excess fat storage in rat hepatocytes. J Hepatol. 2011;54(6):1230-6.
Grasselli, E., Voci, A., Canesi, L., De Matteis, R., Goglia, F., Cioffi, F., Fugassa, E., Gallo, G., & Vergani, L. (2011). Direct effects of iodothyronines on excess fat storage in rat hepatocytes. Journal of Hepatology, 54(6), 1230-6. https://doi.org/10.1016/j.jhep.2010.09.027
Grasselli E, et al. Direct Effects of Iodothyronines On Excess Fat Storage in Rat Hepatocytes. J Hepatol. 2011;54(6):1230-6. PubMed PMID: 21145833.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct effects of iodothyronines on excess fat storage in rat hepatocytes. AU - Grasselli,Elena, AU - Voci,Adriana, AU - Canesi,Laura, AU - De Matteis,Rita, AU - Goglia,Fernando, AU - Cioffi,Federica, AU - Fugassa,Emilia, AU - Gallo,Gabriella, AU - Vergani,Laura, Y1 - 2010/11/03/ PY - 2010/04/30/received PY - 2010/08/16/revised PY - 2010/09/19/accepted PY - 2010/12/15/entrez PY - 2010/12/15/pubmed PY - 2011/9/29/medline SP - 1230 EP - 6 JF - Journal of hepatology JO - J Hepatol VL - 54 IS - 6 N2 - BACKGROUND & AIMS: Previous studies have demonstrated that 3,5-L-diiodothyronine (T(2)) is able to prevent lipid accumulation in the liver of rats fed a high-fat diet. Whether this effect is due to a direct action of T(2) on the liver has not been elucidated. In this study, we investigated the ability of T(2) to reduce the excess lipids in isolated hepatocytes treated with fatty acids (FFAs). The effects of T(2) were compared with those elicited by 3,3',5-L-triiodothyronine (T(3)). METHODS: To mimic the fatty liver condition, primary cultures of rat hepatocytes were overloaded with lipids, by exposure to FFAs ("fatty hepatocytes"), and then treated with T(2) or T(3). Lipid content, morphometry of lipid droplets (LDs), and expression of the adipocyte differentiation-related protein (ADRP) and the peroxisome proliferator-activated receptors (PPAR-α, -γ, -δ) were evaluated. Activities of the lipolytic enzyme acyl CoA oxidase-AOX and the antioxidant enzymes superoxide dismutase-SOD and catalase-CAT were also determined. RESULTS: FFA-induced lipid accumulation was associated with an increase in both number/size of LDs and expression of ADRP, PPAR-γ, and PPAR-δ/β mRNAs, as well as in the activities of AOX, SOD, and CAT. The addition of T(2) or T(3) to "fatty hepatocytes" resulted in a reduction in: (i) lipid content and LD diameter; (ii) PPAR-γ and PPAR-δ expression; (iii) activities of AOX and antioxidant enzymes. CONCLUSIONS: These data demonstrate, for the first time, a direct action of both T(2) and T(3) in reducing the excess fat in cultured hepatocytes. SN - 1600-0641 UR - https://www.unboundmedicine.com/medline/citation/21145833/Direct_effects_of_iodothyronines_on_excess_fat_storage_in_rat_hepatocytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-8278(10)00935-9 DB - PRIME DP - Unbound Medicine ER -