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Comparative neuroanatomical and temporal characterization of FluoroJade-positive neurodegeneration after status epilepticus induced by systemic and intrahippocampal pilocarpine in Wistar rats.
Brain Res. 2011 Feb 16; 1374:43-55.BR

Abstract

The aims of this study were to characterize the spatial distribution of neurodegeneration after status epilepticus (SE) induced by either systemic (S) or intrahippocampal (H) injection of pilocarpine (PILO), two models of temporal lobe epilepsy (TLE), using FluoroJade (FJ) histochemistry, and to evaluate the kinetics of FJ staining in the H-PILO model. Therefore, we measured the severity of behavioral seizures during both types of SE and also evaluated the FJ staining pattern at 12, 24, and 168 h (7days) after the H-PILO insult. We found that the amount of FJ-positive (FJ+) area was greater in SE induced by S-PILO as compared to SE induced by H-PILO. After SE induced by H-PILO, we found more FJ+ cells in the hilus of the dentate gyrus (DG) at 12 h, in CA3 at 24 h, and in CA1 at 168 h. We found also no correlation between seizure severity and the number of FJ+ cells in the hippocampus. Co-localization studies of FJ+ cells with either neuronal-specific nuclear protein (NeuN) or glial fibrillary acidic protein (GFAP) labeling 24 h after H-PILO demonstrated spatially selective neurodegeneration. Double labeling with FJ and parvalbumin (PV) showed both FJ+/PV+ and FJ+/PV- cells in hippocampus and entorhinal cortex, among other areas. The current data indicate that FJ+ areas are differentially distributed in the two TLE models and that these areas are greater in the S-PILO than in the H-PILO model. There is also a selective kinetics of FJ+ cells in the hippocampus after SE induced by H-PILO, with no association with the severity of seizures, probably as a consequence of the extra-hippocampal damage. These data point to SE induced by H-PILO as a low-mortality model of TLE, with regional spatial and temporal patterns of FJ staining.

Authors+Show Affiliations

Physiology Department, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21147076

Citation

Castro, O W., et al. "Comparative Neuroanatomical and Temporal Characterization of FluoroJade-positive Neurodegeneration After Status Epilepticus Induced By Systemic and Intrahippocampal Pilocarpine in Wistar Rats." Brain Research, vol. 1374, 2011, pp. 43-55.
Castro OW, Furtado MA, Tilelli CQ, et al. Comparative neuroanatomical and temporal characterization of FluoroJade-positive neurodegeneration after status epilepticus induced by systemic and intrahippocampal pilocarpine in Wistar rats. Brain Res. 2011;1374:43-55.
Castro, O. W., Furtado, M. A., Tilelli, C. Q., Fernandes, A., Pajolla, G. P., & Garcia-Cairasco, N. (2011). Comparative neuroanatomical and temporal characterization of FluoroJade-positive neurodegeneration after status epilepticus induced by systemic and intrahippocampal pilocarpine in Wistar rats. Brain Research, 1374, 43-55. https://doi.org/10.1016/j.brainres.2010.12.012
Castro OW, et al. Comparative Neuroanatomical and Temporal Characterization of FluoroJade-positive Neurodegeneration After Status Epilepticus Induced By Systemic and Intrahippocampal Pilocarpine in Wistar Rats. Brain Res. 2011 Feb 16;1374:43-55. PubMed PMID: 21147076.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative neuroanatomical and temporal characterization of FluoroJade-positive neurodegeneration after status epilepticus induced by systemic and intrahippocampal pilocarpine in Wistar rats. AU - Castro,O W, AU - Furtado,M A, AU - Tilelli,C Q, AU - Fernandes,A, AU - Pajolla,G P, AU - Garcia-Cairasco,N, Y1 - 2010/12/11/ PY - 2010/06/29/received PY - 2010/12/02/revised PY - 2010/12/04/accepted PY - 2010/12/15/entrez PY - 2010/12/15/pubmed PY - 2011/12/14/medline SP - 43 EP - 55 JF - Brain research JO - Brain Res. VL - 1374 N2 - The aims of this study were to characterize the spatial distribution of neurodegeneration after status epilepticus (SE) induced by either systemic (S) or intrahippocampal (H) injection of pilocarpine (PILO), two models of temporal lobe epilepsy (TLE), using FluoroJade (FJ) histochemistry, and to evaluate the kinetics of FJ staining in the H-PILO model. Therefore, we measured the severity of behavioral seizures during both types of SE and also evaluated the FJ staining pattern at 12, 24, and 168 h (7days) after the H-PILO insult. We found that the amount of FJ-positive (FJ+) area was greater in SE induced by S-PILO as compared to SE induced by H-PILO. After SE induced by H-PILO, we found more FJ+ cells in the hilus of the dentate gyrus (DG) at 12 h, in CA3 at 24 h, and in CA1 at 168 h. We found also no correlation between seizure severity and the number of FJ+ cells in the hippocampus. Co-localization studies of FJ+ cells with either neuronal-specific nuclear protein (NeuN) or glial fibrillary acidic protein (GFAP) labeling 24 h after H-PILO demonstrated spatially selective neurodegeneration. Double labeling with FJ and parvalbumin (PV) showed both FJ+/PV+ and FJ+/PV- cells in hippocampus and entorhinal cortex, among other areas. The current data indicate that FJ+ areas are differentially distributed in the two TLE models and that these areas are greater in the S-PILO than in the H-PILO model. There is also a selective kinetics of FJ+ cells in the hippocampus after SE induced by H-PILO, with no association with the severity of seizures, probably as a consequence of the extra-hippocampal damage. These data point to SE induced by H-PILO as a low-mortality model of TLE, with regional spatial and temporal patterns of FJ staining. SN - 1872-6240 UR - https://www.unboundmedicine.com/medline/citation/21147076/Comparative_neuroanatomical_and_temporal_characterization_of_FluoroJade_positive_neurodegeneration_after_status_epilepticus_induced_by_systemic_and_intrahippocampal_pilocarpine_in_Wistar_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(10)02651-X DB - PRIME DP - Unbound Medicine ER -