Tags

Type your tag names separated by a space and hit enter

The periplasmic chaperone Skp is required for successful Salmonella Typhimurium infection in a murine typhoid model.
Microbiology (Reading). 2011 Mar; 157(Pt 3):848-858.M

Abstract

The alternative sigma factor σ(E) (rpoE) is essential for survival in vivo of Salmonella Typhimurium but is dispensable during growth in the laboratory. We have been identifying σ(E)-regulated genes and studying their regulation and function to elucidate their potential role in the severe attenuation of S. Typhimurium rpoE mutants. In this study we identify five promoters that control the rseP, yaeT (bamA), skp region. A confirmed σ(E)-dependent promoter, yaeTp1, and a second downstream promoter, yaeTp2, are located within the upstream gene rseP and direct expression of the downstream genes. The only known function of RseP is σ(E) activation, and it is therefore not expected to be essential for S. Typhimurium in vitro. However, it proved impossible to delete the entire rseP gene due to the presence of internal promoters that regulate the essential gene yaeT. We could inactivate rseP by deleting the first third of the gene, leaving the yaeT promoters intact. Like the rpoE mutant, the rseP mutant exhibited severe attenuation in vivo. We were able to delete the entire coding sequence of skp, which encodes a periplasmic chaperone involved in targeting misfolded outer-membrane proteins to the β-barrel assembly machinery. The skp mutant was attenuated in mice after oral and parenteral infection. Virulence could be complemented by providing skp in trans but only by linking it to a heterologous σ(E)-regulated promoter. The reason the skp mutant is attenuated is currently enigmatic, but we know it is not through increased sensitivity to a variety of RpoE-activating host stresses, such as H(2)O(2), polymyxin B and high temperature, or through altered secretion of effector proteins by either the Salmonella pathogenicity island (SPI)-1 or the SPI-2 type III secretion system.

Authors+Show Affiliations

School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK.Institute of Molecular Biology, Slovak Academy of Sciences, Dubravska cesta 21, 845 51 Bratislava, Slovak Republik.Institute of Comparative Medicine, Faculty of Veterinary Medicine, University of Glasgow, Bearsden Road, Glasgow G61 1QH, UK.Institute of Molecular Biology, Slovak Academy of Sciences, Dubravska cesta 21, 845 51 Bratislava, Slovak Republik.Institute of Molecular Biology, Slovak Academy of Sciences, Dubravska cesta 21, 845 51 Bratislava, Slovak Republik.Institute of Comparative Medicine, Faculty of Veterinary Medicine, University of Glasgow, Bearsden Road, Glasgow G61 1QH, UK.Institute of Comparative Medicine, Faculty of Veterinary Medicine, University of Glasgow, Bearsden Road, Glasgow G61 1QH, UK.Institute of Molecular Biology, Slovak Academy of Sciences, Dubravska cesta 21, 845 51 Bratislava, Slovak Republik.Institute of Comparative Medicine, Faculty of Veterinary Medicine, University of Glasgow, Bearsden Road, Glasgow G61 1QH, UK.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21148205

Citation

Rowley, Gary, et al. "The Periplasmic Chaperone Skp Is Required for Successful Salmonella Typhimurium Infection in a Murine Typhoid Model." Microbiology (Reading, England), vol. 157, no. Pt 3, 2011, pp. 848-858.
Rowley G, Skovierova H, Stevenson A, et al. The periplasmic chaperone Skp is required for successful Salmonella Typhimurium infection in a murine typhoid model. Microbiology (Reading). 2011;157(Pt 3):848-858.
Rowley, G., Skovierova, H., Stevenson, A., Rezuchova, B., Homerova, D., Lewis, C., Sherry, A., Kormanec, J., & Roberts, M. (2011). The periplasmic chaperone Skp is required for successful Salmonella Typhimurium infection in a murine typhoid model. Microbiology (Reading, England), 157(Pt 3), 848-858. https://doi.org/10.1099/mic.0.046011-0
Rowley G, et al. The Periplasmic Chaperone Skp Is Required for Successful Salmonella Typhimurium Infection in a Murine Typhoid Model. Microbiology (Reading). 2011;157(Pt 3):848-858. PubMed PMID: 21148205.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The periplasmic chaperone Skp is required for successful Salmonella Typhimurium infection in a murine typhoid model. AU - Rowley,Gary, AU - Skovierova,Henrieta, AU - Stevenson,Andrew, AU - Rezuchova,Bronislava, AU - Homerova,Dagmar, AU - Lewis,Claire, AU - Sherry,Aileen, AU - Kormanec,Jan, AU - Roberts,Mark, Y1 - 2010/12/09/ PY - 2010/12/15/entrez PY - 2010/12/15/pubmed PY - 2011/6/18/medline SP - 848 EP - 858 JF - Microbiology (Reading, England) JO - Microbiology (Reading) VL - 157 IS - Pt 3 N2 - The alternative sigma factor σ(E) (rpoE) is essential for survival in vivo of Salmonella Typhimurium but is dispensable during growth in the laboratory. We have been identifying σ(E)-regulated genes and studying their regulation and function to elucidate their potential role in the severe attenuation of S. Typhimurium rpoE mutants. In this study we identify five promoters that control the rseP, yaeT (bamA), skp region. A confirmed σ(E)-dependent promoter, yaeTp1, and a second downstream promoter, yaeTp2, are located within the upstream gene rseP and direct expression of the downstream genes. The only known function of RseP is σ(E) activation, and it is therefore not expected to be essential for S. Typhimurium in vitro. However, it proved impossible to delete the entire rseP gene due to the presence of internal promoters that regulate the essential gene yaeT. We could inactivate rseP by deleting the first third of the gene, leaving the yaeT promoters intact. Like the rpoE mutant, the rseP mutant exhibited severe attenuation in vivo. We were able to delete the entire coding sequence of skp, which encodes a periplasmic chaperone involved in targeting misfolded outer-membrane proteins to the β-barrel assembly machinery. The skp mutant was attenuated in mice after oral and parenteral infection. Virulence could be complemented by providing skp in trans but only by linking it to a heterologous σ(E)-regulated promoter. The reason the skp mutant is attenuated is currently enigmatic, but we know it is not through increased sensitivity to a variety of RpoE-activating host stresses, such as H(2)O(2), polymyxin B and high temperature, or through altered secretion of effector proteins by either the Salmonella pathogenicity island (SPI)-1 or the SPI-2 type III secretion system. SN - 1465-2080 UR - https://www.unboundmedicine.com/medline/citation/21148205/The_periplasmic_chaperone_Skp_is_required_for_successful_Salmonella_Typhimurium_infection_in_a_murine_typhoid_model_ L2 - http://mic.microbiologyresearch.org/pubmed/content/journal/micro/10.1099/mic.0.046011-0 DB - PRIME DP - Unbound Medicine ER -