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Molecular analysis of swine hepatitis E virus from north India.
Indian J Med Res. 2010 Nov; 132:504-8.IJ

Abstract

BACKGROUND & OBJECTIVES

Hepatitis E is the main cause of enterically transmitted non-A, non-B hepatitis in developing countries. In the developed countries such as the USA, Japan and Taiwan, the viruses infecting humans and swine share the same genotype with a high sequence similarity. Genotype 1 circulates in humans whereas genotype 4 in pigs in India. The present study was designed to investigate the presence of anti-HEV antibodies and HEV-RNA in swine population from north India, to investigate the genotype prevalent in it, and to compare it with other swine and human HEV strains from India.

METHODS

A total of 67 serum samples were collected from pigs of age period (1-6 months) from Indian Veterinary Research Institute (IVRI), Izatnagar, Bareily and subjected to anti-HEV IgG and HEV RNA detection. A phylogenetic tree was constructed using the neighbor-joining method and evaluated using the interior branch test method with MEGA 4 software.

RESULTS

Anti-HEV IgG and HEV RNA was found in 38.8 and 4.5 per cent of swine samples studied respectively. The above samples were observed to be of genotype 4e. The three new sequences had nucleotide similarity with other swine sequences in genotype 4 ranging from 80-98 per cent.

INTERPRETATION & CONCLUSIONS

The three sequences observed in the present study showed nucleotide similarity with other swine sequences from southern and western India. The present study suggests that genotype 4 'e' is prevalent in the north India.

Authors+Show Affiliations

Department of Medicine, Maulana Azad Medical College, University of Delhi, New Delhi, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21149998

Citation

Begum, Nargis, et al. "Molecular Analysis of Swine Hepatitis E Virus From North India." The Indian Journal of Medical Research, vol. 132, 2010, pp. 504-8.
Begum N, Polipalli SK, Husain SA, et al. Molecular analysis of swine hepatitis E virus from north India. Indian J Med Res. 2010;132:504-8.
Begum, N., Polipalli, S. K., Husain, S. A., & Kar, P. (2010). Molecular analysis of swine hepatitis E virus from north India. The Indian Journal of Medical Research, 132, 504-8.
Begum N, et al. Molecular Analysis of Swine Hepatitis E Virus From North India. Indian J Med Res. 2010;132:504-8. PubMed PMID: 21149998.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular analysis of swine hepatitis E virus from north India. AU - Begum,Nargis, AU - Polipalli,Sunil K, AU - Husain,Syed A, AU - Kar,Premashis, PY - 2010/12/15/entrez PY - 2010/12/15/pubmed PY - 2011/3/30/medline SP - 504 EP - 8 JF - The Indian journal of medical research JO - Indian J Med Res VL - 132 N2 - BACKGROUND & OBJECTIVES: Hepatitis E is the main cause of enterically transmitted non-A, non-B hepatitis in developing countries. In the developed countries such as the USA, Japan and Taiwan, the viruses infecting humans and swine share the same genotype with a high sequence similarity. Genotype 1 circulates in humans whereas genotype 4 in pigs in India. The present study was designed to investigate the presence of anti-HEV antibodies and HEV-RNA in swine population from north India, to investigate the genotype prevalent in it, and to compare it with other swine and human HEV strains from India. METHODS: A total of 67 serum samples were collected from pigs of age period (1-6 months) from Indian Veterinary Research Institute (IVRI), Izatnagar, Bareily and subjected to anti-HEV IgG and HEV RNA detection. A phylogenetic tree was constructed using the neighbor-joining method and evaluated using the interior branch test method with MEGA 4 software. RESULTS: Anti-HEV IgG and HEV RNA was found in 38.8 and 4.5 per cent of swine samples studied respectively. The above samples were observed to be of genotype 4e. The three new sequences had nucleotide similarity with other swine sequences in genotype 4 ranging from 80-98 per cent. INTERPRETATION & CONCLUSIONS: The three sequences observed in the present study showed nucleotide similarity with other swine sequences from southern and western India. The present study suggests that genotype 4 'e' is prevalent in the north India. SN - 0971-5916 UR - https://www.unboundmedicine.com/medline/citation/21149998/Molecular_analysis_of_swine_hepatitis_E_virus_from_north_India_ L2 - http://www.ijmr.org.in/article.asp?issn=0971-5916;year=2010;volume=132;issue=5;spage=504;epage=508;aulast=Begum;type=2 DB - PRIME DP - Unbound Medicine ER -