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Comparative expression of matrix-associated genes and inflammatory cytokines-associated genes according to disc degeneration: analysis of living human nucleus pulposus.
J Spinal Disord Tech. 2011 Aug; 24(6):352-7.JS

Abstract

STUDY DESIGN

The 2 groups of living human nucleus pulposus were prospectively compared according to disc degeneration.

OBJECTIVES

This study was conducted to investigate the expressions of various genes associated with matrix synthesis and expressions of inflammatory cytokines-associated genes according to degrees of disc degeneration in human discs.

SUMMARY OF BACKGROUND DATA

Degenerated discs were obtained from 18 patients who underwent discectomy for lumbar disc herniation. Disc degeneration was graded by T2-weighted magnetic resonance imaging using Pfirrmann's grading system. Discs were allocated to 2 groups: group I (9 patients)-mildly degenerated discs (grades II and III) and group II (9 patients)-severely degenerated discs (grades IV and V).

METHODS

Cells from the nucleus pulposus were isolated and then cultured as monolayers. The mRNA expressions of aggrecan, type II collagen, Sox9, type I collagen, alkaline phosphatase, osteocalcin, tumor necrosis factor-α, and interleukin-1β in the 2 groups were compared by real-time polymerase chain reaction, and production of matrix-associate proteins (aggrecan, type II collagen, Sox9, type I collagen, alkaline phosphatase, and osteocalcin) were compared by Western blotting.

RESULTS

mRNA expressions in group I were upregulated versus group II to the following extents: 1.83 times for aggrecan, 1.82 times for type II collagen, 1.80 times for Sox9, 1.41 times for type I collagen, 1.38 times for alkaline phosphatase, and 1.80 times for osteocalcin. Furthermore, Western blotting showed that aggrecan, type II collagen, Sox9, type I collagen, alkaline phosphatase, and osteocalcin were higher in group I. However, the mRNA levels of tumor necrosis factor-α and interleukin-1β were 1.26 and 1.11-fold, respectively, upregulated in group II.

CONCLUSIONS

Mildly degenerated discs showed greater matrix, chondrogenic, and osteoblastic gene expressions than severely degenerated discs, indicating that the ability to produce matrix-associated proteins is greater for cells in mildly degenerated than in severely degenerated discs. However, inflammatory cytokine genes associated with disc degeneration were expressed at higher levels in the severely degenerated group. This study shows that a reduction in matrix synthesis and an increase of inflammatory cytokine levels occurs during disc degeneration at the same time.

Authors+Show Affiliations

Department of Neurosurgery, Spine and Spinal Cord Institute, Gangnam Severance Spine Hospital, Yonsei University College of Medicine, 146-92 Dogok-dong, Kangnam Gu, Seoul, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21150669

Citation

Park, Jeong Yoon, et al. "Comparative Expression of Matrix-associated Genes and Inflammatory Cytokines-associated Genes According to Disc Degeneration: Analysis of Living Human Nucleus Pulposus." Journal of Spinal Disorders & Techniques, vol. 24, no. 6, 2011, pp. 352-7.
Park JY, Kuh SU, Park HS, et al. Comparative expression of matrix-associated genes and inflammatory cytokines-associated genes according to disc degeneration: analysis of living human nucleus pulposus. J Spinal Disord Tech. 2011;24(6):352-7.
Park, J. Y., Kuh, S. U., Park, H. S., & Kim, K. S. (2011). Comparative expression of matrix-associated genes and inflammatory cytokines-associated genes according to disc degeneration: analysis of living human nucleus pulposus. Journal of Spinal Disorders & Techniques, 24(6), 352-7. https://doi.org/10.1097/BSD.0b013e3181fee4df
Park JY, et al. Comparative Expression of Matrix-associated Genes and Inflammatory Cytokines-associated Genes According to Disc Degeneration: Analysis of Living Human Nucleus Pulposus. J Spinal Disord Tech. 2011;24(6):352-7. PubMed PMID: 21150669.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative expression of matrix-associated genes and inflammatory cytokines-associated genes according to disc degeneration: analysis of living human nucleus pulposus. AU - Park,Jeong Yoon, AU - Kuh,Sung Uk, AU - Park,Hyo Suk, AU - Kim,Keun Su, PY - 2010/12/15/entrez PY - 2010/12/15/pubmed PY - 2011/12/13/medline SP - 352 EP - 7 JF - Journal of spinal disorders & techniques JO - J Spinal Disord Tech VL - 24 IS - 6 N2 - STUDY DESIGN: The 2 groups of living human nucleus pulposus were prospectively compared according to disc degeneration. OBJECTIVES: This study was conducted to investigate the expressions of various genes associated with matrix synthesis and expressions of inflammatory cytokines-associated genes according to degrees of disc degeneration in human discs. SUMMARY OF BACKGROUND DATA: Degenerated discs were obtained from 18 patients who underwent discectomy for lumbar disc herniation. Disc degeneration was graded by T2-weighted magnetic resonance imaging using Pfirrmann's grading system. Discs were allocated to 2 groups: group I (9 patients)-mildly degenerated discs (grades II and III) and group II (9 patients)-severely degenerated discs (grades IV and V). METHODS: Cells from the nucleus pulposus were isolated and then cultured as monolayers. The mRNA expressions of aggrecan, type II collagen, Sox9, type I collagen, alkaline phosphatase, osteocalcin, tumor necrosis factor-α, and interleukin-1β in the 2 groups were compared by real-time polymerase chain reaction, and production of matrix-associate proteins (aggrecan, type II collagen, Sox9, type I collagen, alkaline phosphatase, and osteocalcin) were compared by Western blotting. RESULTS: mRNA expressions in group I were upregulated versus group II to the following extents: 1.83 times for aggrecan, 1.82 times for type II collagen, 1.80 times for Sox9, 1.41 times for type I collagen, 1.38 times for alkaline phosphatase, and 1.80 times for osteocalcin. Furthermore, Western blotting showed that aggrecan, type II collagen, Sox9, type I collagen, alkaline phosphatase, and osteocalcin were higher in group I. However, the mRNA levels of tumor necrosis factor-α and interleukin-1β were 1.26 and 1.11-fold, respectively, upregulated in group II. CONCLUSIONS: Mildly degenerated discs showed greater matrix, chondrogenic, and osteoblastic gene expressions than severely degenerated discs, indicating that the ability to produce matrix-associated proteins is greater for cells in mildly degenerated than in severely degenerated discs. However, inflammatory cytokine genes associated with disc degeneration were expressed at higher levels in the severely degenerated group. This study shows that a reduction in matrix synthesis and an increase of inflammatory cytokine levels occurs during disc degeneration at the same time. SN - 1539-2465 UR - https://www.unboundmedicine.com/medline/citation/21150669/Comparative_expression_of_matrix_associated_genes_and_inflammatory_cytokines_associated_genes_according_to_disc_degeneration:_analysis_of_living_human_nucleus_pulposus_ L2 - http://dx.doi.org/10.1097/BSD.0b013e3181fee4df DB - PRIME DP - Unbound Medicine ER -