TY - JOUR T1 - Phylogenetic and phyletic studies of informational genes in genomes highlight existence of a 4 domain of life including giant viruses. AU - Boyer,Mickaël, AU - Madoui,Mohammed-Amine, AU - Gimenez,Gregory, AU - La Scola,Bernard, AU - Raoult,Didier, Y1 - 2010/12/02/ PY - 2010/08/04/received PY - 2010/10/11/accepted PY - 2010/12/15/entrez PY - 2010/12/15/pubmed PY - 2011/7/6/medline SP - e15530 EP - e15530 JF - PloS one JO - PLoS One VL - 5 IS - 12 N2 - The discovery of Mimivirus, with its very large genome content, made it possible to identify genes common to the three domains of life (Eukarya, Bacteria and Archaea) and to generate controversial phylogenomic trees congruent with that of ribosomal genes, branching Mimivirus at its root. Here we used sequences from metagenomic databases, Marseillevirus and three new viruses extending the Mimiviridae family to generate the phylogenetic trees of eight proteins involved in different steps of DNA processing. Compared to the three ribosomal defined domains, we report a single common origin for Nucleocytoplasmic Large DNA Viruses (NCLDV), DNA processing genes rooted between Archaea and Eukarya, with a topology congruent with that of the ribosomal tree. As for translation, we found in our new viruses, together with Mimivirus, five proteins rooted deeply in the eukaryotic clade. In addition, comparison of informational genes repertoire based on phyletic pattern analysis supports existence of a clade containing NCLDVs clearly distinct from that of Eukarya, Bacteria and Archaea. We hypothesize that the core genome of NCLDV is as ancient as the three currently accepted domains of life. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/21151962/Phylogenetic_and_phyletic_studies_of_informational_genes_in_genomes_highlight_existence_of_a_4_domain_of_life_including_giant_viruses_ L2 - https://dx.plos.org/10.1371/journal.pone.0015530 DB - PRIME DP - Unbound Medicine ER -