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Distribution of endogenous farnesyl pyrophosphate and four species of lysophosphatidic acid in rodent brain.
Int J Mol Sci. 2010 Oct 15; 11(10):3965-76.IJ

Abstract

Lysophosphatidic acid (LPA) is the umbrella term for lipid signaling molecules that share structural homology and activate the family of LPA receptors. Farnesyl Pyrophosphate (FPP) is commonly known as an intermediate in the synthesis of steroid hormones; however, its function as a signaling lipid is beginning to be explored. FPP was recently shown to an activator of the G-protein coupled receptor 92 (also known as LPA5) of the calcium channel TRPV(3). The LPA receptors (including GPR92) are associated with the signal transduction of noxious stimuli, however, very little is known about the distribution of their signaling ligands (LPAs and FPP) in the brain. Here, using HPLC/MS/MS, we developed extraction and analytical methods for measuring levels of FPP and 4 species of LPA (palmitoyl, stearoyl, oleoyl and arachidonoyl-sn-glycerol-3 phosphate) in rodent brain. Relative distributions of each of the five compounds was significantly different across the brain suggesting divergent functionality for each as signaling molecules based on where and how much of each is being produced. Brainstem, midbrain, and thalamus contained the highest levels measured for each compound, though none in the same ratios while relatively small amounts were produced in cortex and cerebellum. These data provide a framework for investigations into functional relationships of these lipid ligands in specific brain areas, many of which are associated with the perception of pain.

Authors+Show Affiliations

The Department of Psychological and Brain Sciences, Indiana University Bloomington, IN 47405, USA; E-Mails: sunghalee@gmail.com (S.H.L); sraboune@indiana.edu (S.R.); mtheodor@indiana.edu (J.M.W.).No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21152313

Citation

Lee, Sung Ha, et al. "Distribution of Endogenous Farnesyl Pyrophosphate and Four Species of Lysophosphatidic Acid in Rodent Brain." International Journal of Molecular Sciences, vol. 11, no. 10, 2010, pp. 3965-76.
Lee SH, Raboune S, Walker JM, et al. Distribution of endogenous farnesyl pyrophosphate and four species of lysophosphatidic acid in rodent brain. Int J Mol Sci. 2010;11(10):3965-76.
Lee, S. H., Raboune, S., Walker, J. M., & Bradshaw, H. B. (2010). Distribution of endogenous farnesyl pyrophosphate and four species of lysophosphatidic acid in rodent brain. International Journal of Molecular Sciences, 11(10), 3965-76. https://doi.org/10.3390/ijms11103965
Lee SH, et al. Distribution of Endogenous Farnesyl Pyrophosphate and Four Species of Lysophosphatidic Acid in Rodent Brain. Int J Mol Sci. 2010 Oct 15;11(10):3965-76. PubMed PMID: 21152313.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distribution of endogenous farnesyl pyrophosphate and four species of lysophosphatidic acid in rodent brain. AU - Lee,Sung Ha, AU - Raboune,Siham, AU - Walker,J Michael, AU - Bradshaw,Heather B, Y1 - 2010/10/15/ PY - 2010/08/24/received PY - 2010/10/12/revised PY - 2010/10/13/accepted PY - 2010/12/15/entrez PY - 2010/12/15/pubmed PY - 2010/12/15/medline KW - FPP KW - GPR92 KW - LC/MS/MS KW - LPA KW - TRPV3 KW - pain SP - 3965 EP - 76 JF - International journal of molecular sciences JO - Int J Mol Sci VL - 11 IS - 10 N2 - Lysophosphatidic acid (LPA) is the umbrella term for lipid signaling molecules that share structural homology and activate the family of LPA receptors. Farnesyl Pyrophosphate (FPP) is commonly known as an intermediate in the synthesis of steroid hormones; however, its function as a signaling lipid is beginning to be explored. FPP was recently shown to an activator of the G-protein coupled receptor 92 (also known as LPA5) of the calcium channel TRPV(3). The LPA receptors (including GPR92) are associated with the signal transduction of noxious stimuli, however, very little is known about the distribution of their signaling ligands (LPAs and FPP) in the brain. Here, using HPLC/MS/MS, we developed extraction and analytical methods for measuring levels of FPP and 4 species of LPA (palmitoyl, stearoyl, oleoyl and arachidonoyl-sn-glycerol-3 phosphate) in rodent brain. Relative distributions of each of the five compounds was significantly different across the brain suggesting divergent functionality for each as signaling molecules based on where and how much of each is being produced. Brainstem, midbrain, and thalamus contained the highest levels measured for each compound, though none in the same ratios while relatively small amounts were produced in cortex and cerebellum. These data provide a framework for investigations into functional relationships of these lipid ligands in specific brain areas, many of which are associated with the perception of pain. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/21152313/Distribution_of_endogenous_farnesyl_pyrophosphate_and_four_species_of_lysophosphatidic_acid_in_rodent_brain_ L2 - https://doi.org/10.3390/ijms11103965 DB - PRIME DP - Unbound Medicine ER -