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Human peritoneal mesothelial cell transformation into myofibroblasts in response to TGF-β1 in vitro.
Int J Mol Med. 2011 Feb; 27(2):187-93.IJ

Abstract

Peritoneal dissemination is one of the leading causes of death in gastric cancer patients. The interaction between carcinoma cells and the peritoneal lining may play a key role in tumor peritoneal dissemination. Human peritoneal mesothelial cells are a monolayer of squamous epithelial cells covering the peritoneal cavity and forming serosal membranes. The precise role of mesothelial cells in the peritoneal dissemination of gastric cancer remains to be identified. Expression of TGF-β1, a cytokine known for its capacity to induce proliferative and transformative changes in cells, has been correlated with peritoneal metastasis and TNM stages of gastric cancer. High levels of TGF-β1 in the subperitoneal milieu may play a key role in the transition of normal mesothelial cells to myofibroblasts. Here, we demonstrate that mesothelial cells activated by TGF-β1 undergo epithelial-mesenchymal transition (EMT) and that the transition of mesothelial cells to myofibroblasts is dependent on Smad2 signaling. EMT of mesothelial cells was marked by up-regulation of α-smooth muscle actin and vimentin expression. Cytokeratin and E-cadherin expression decreased over time in transformed mesothelial cells. Knockdown of Smad2 gene by siRNA silencing significantly suppressed the transition of mesothelial cells to myofibroblasts. We conclude that when exposed to TGF-β1 mesothelial cells undergo EMT which involves Smad2 signaling. Furthermore, mesothelial cells may be the possible source of myofibroblasts in peritoneal fibrosis and provide a favorable environment for the dissemination of gastric cancer.

Authors+Show Affiliations

Department of Surgical Oncology, The First Affiliated Hospital, China Medical University, Shenyang, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21152863

Citation

Lv, Zhi-Dong, et al. "Human Peritoneal Mesothelial Cell Transformation Into Myofibroblasts in Response to TGF-β1 in Vitro." International Journal of Molecular Medicine, vol. 27, no. 2, 2011, pp. 187-93.
Lv ZD, Na D, Ma XY, et al. Human peritoneal mesothelial cell transformation into myofibroblasts in response to TGF-β1 in vitro. Int J Mol Med. 2011;27(2):187-93.
Lv, Z. D., Na, D., Ma, X. Y., Zhao, C., Zhao, W. J., & Xu, H. M. (2011). Human peritoneal mesothelial cell transformation into myofibroblasts in response to TGF-β1 in vitro. International Journal of Molecular Medicine, 27(2), 187-93. https://doi.org/10.3892/ijmm.2010.574
Lv ZD, et al. Human Peritoneal Mesothelial Cell Transformation Into Myofibroblasts in Response to TGF-β1 in Vitro. Int J Mol Med. 2011;27(2):187-93. PubMed PMID: 21152863.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human peritoneal mesothelial cell transformation into myofibroblasts in response to TGF-β1 in vitro. AU - Lv,Zhi-Dong, AU - Na,Di, AU - Ma,Xiao-Yang, AU - Zhao,Chong, AU - Zhao,Wei-Jun, AU - Xu,Hui-Mian, Y1 - 2010/12/06/ PY - 2010/08/22/received PY - 2010/10/13/accepted PY - 2010/12/15/entrez PY - 2010/12/15/pubmed PY - 2011/4/1/medline SP - 187 EP - 93 JF - International journal of molecular medicine JO - Int J Mol Med VL - 27 IS - 2 N2 - Peritoneal dissemination is one of the leading causes of death in gastric cancer patients. The interaction between carcinoma cells and the peritoneal lining may play a key role in tumor peritoneal dissemination. Human peritoneal mesothelial cells are a monolayer of squamous epithelial cells covering the peritoneal cavity and forming serosal membranes. The precise role of mesothelial cells in the peritoneal dissemination of gastric cancer remains to be identified. Expression of TGF-β1, a cytokine known for its capacity to induce proliferative and transformative changes in cells, has been correlated with peritoneal metastasis and TNM stages of gastric cancer. High levels of TGF-β1 in the subperitoneal milieu may play a key role in the transition of normal mesothelial cells to myofibroblasts. Here, we demonstrate that mesothelial cells activated by TGF-β1 undergo epithelial-mesenchymal transition (EMT) and that the transition of mesothelial cells to myofibroblasts is dependent on Smad2 signaling. EMT of mesothelial cells was marked by up-regulation of α-smooth muscle actin and vimentin expression. Cytokeratin and E-cadherin expression decreased over time in transformed mesothelial cells. Knockdown of Smad2 gene by siRNA silencing significantly suppressed the transition of mesothelial cells to myofibroblasts. We conclude that when exposed to TGF-β1 mesothelial cells undergo EMT which involves Smad2 signaling. Furthermore, mesothelial cells may be the possible source of myofibroblasts in peritoneal fibrosis and provide a favorable environment for the dissemination of gastric cancer. SN - 1791-244X UR - https://www.unboundmedicine.com/medline/citation/21152863/Human_peritoneal_mesothelial_cell_transformation_into_myofibroblasts_in_response_to_TGF_��1_in_vitro_ L2 - http://www.spandidos-publications.com/ijmm/27/2/187 DB - PRIME DP - Unbound Medicine ER -