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[Intrathecal injection of MK-801 inhibited the NOS activity and NO content of hippocampus in rat during the process of formalin-induced inflammatory pain].
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2009 Aug; 25(3):423-7.ZY

Abstract

AIM

To study the effect of intrathecal injection of MK-801, a NMDA receptor antagonist, on the NOS activity and NO content of hippocampus in rat during the process of formalin-induced inflammatory pain as well as the pain behavior of rat.

METHODS

The degree of pain was determined by observing the time of licking and biting the injected paw. NOS expression in the hippocampus was determined by using NADPH-d histochemical staining. NO content of hippocampus was determined by assaying NO3; and NO2.

RESULTS

Subcutaneous injection of formalin elicited a characteristic pain behavioural response consisting of licking and biting the injected paw, etc. Intrathecal injection of MK-801 could shorten obviously the time of licking and biting representing pain behavioural response in phase 2. It is suggested that intrathecal injection of MK-801 could block the pain behavioural response induced by formalin (P < 0.05). The number and staining degree of NADPH-d positive neurons in formalin group significantly increased at 12 h after the formalin injection in CA1, CA2-3 and DG of hippocampus compared with control group as well as NO content, however, the number and staining degree of NADPH-d positive neurons in formalin + MK-801 group significantly decreased in contrast to those of formalin 12 h group as well as the NO content (P < 0.01).

CONCLUSION

Intrathecal injection of NMDA receptor antagonist MK-801 could inhibit the NOS activity and NO production in hippocampus of rat, which showed the increase of hippocampal NO production was mainly induced by the peripheral nociceptive information input.

Authors+Show Affiliations

Liu LY
Department of Pathophysiology, Basic Medical college, Hebei Medical University, Shijiazhuang 050017, China.
Li QJ
No affiliation info available
Hu YY
No affiliation info available
Guo XH
No affiliation info available
Chu SC
No affiliation info available
Li WB
No affiliation info available

MeSH

AnimalsDizocilpine MaleateFormaldehydeHippocampusInflammationInjections, SpinalMaleNitric OxideNitric Oxide Synthase Type IPainRandom AllocationRatsRats, Sprague-DawleyReceptors, N-Methyl-D-Aspartate

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

21155253

Citation

Liu, Ling-yun, et al. "[Intrathecal Injection of MK-801 Inhibited the NOS Activity and NO Content of Hippocampus in Rat During the Process of Formalin-induced Inflammatory Pain]." Zhongguo Ying Yong Sheng Li Xue Za Zhi = Zhongguo Yingyong Shenglixue Zazhi = Chinese Journal of Applied Physiology, vol. 25, no. 3, 2009, pp. 423-7.
Liu LY, Li QJ, Hu YY, et al. [Intrathecal injection of MK-801 inhibited the NOS activity and NO content of hippocampus in rat during the process of formalin-induced inflammatory pain]. Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2009;25(3):423-7.
Liu, L. Y., Li, Q. J., Hu, Y. Y., Guo, X. H., Chu, S. C., & Li, W. B. (2009). [Intrathecal injection of MK-801 inhibited the NOS activity and NO content of hippocampus in rat during the process of formalin-induced inflammatory pain]. Zhongguo Ying Yong Sheng Li Xue Za Zhi = Zhongguo Yingyong Shenglixue Zazhi = Chinese Journal of Applied Physiology, 25(3), 423-7.
Liu LY, et al. [Intrathecal Injection of MK-801 Inhibited the NOS Activity and NO Content of Hippocampus in Rat During the Process of Formalin-induced Inflammatory Pain]. Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2009;25(3):423-7. PubMed PMID: 21155253.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Intrathecal injection of MK-801 inhibited the NOS activity and NO content of hippocampus in rat during the process of formalin-induced inflammatory pain]. AU - Liu,Ling-yun, AU - Li,Qing-jun, AU - Hu,Yu-yan, AU - Guo,Xin-hua, AU - Chu,Sai-chun, AU - Li,Wen-bin, PY - 2010/12/16/entrez PY - 2009/8/1/pubmed PY - 2013/1/11/medline SP - 423 EP - 7 JF - Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology JO - Zhongguo Ying Yong Sheng Li Xue Za Zhi VL - 25 IS - 3 N2 - AIM: To study the effect of intrathecal injection of MK-801, a NMDA receptor antagonist, on the NOS activity and NO content of hippocampus in rat during the process of formalin-induced inflammatory pain as well as the pain behavior of rat. METHODS: The degree of pain was determined by observing the time of licking and biting the injected paw. NOS expression in the hippocampus was determined by using NADPH-d histochemical staining. NO content of hippocampus was determined by assaying NO3; and NO2. RESULTS: Subcutaneous injection of formalin elicited a characteristic pain behavioural response consisting of licking and biting the injected paw, etc. Intrathecal injection of MK-801 could shorten obviously the time of licking and biting representing pain behavioural response in phase 2. It is suggested that intrathecal injection of MK-801 could block the pain behavioural response induced by formalin (P < 0.05). The number and staining degree of NADPH-d positive neurons in formalin group significantly increased at 12 h after the formalin injection in CA1, CA2-3 and DG of hippocampus compared with control group as well as NO content, however, the number and staining degree of NADPH-d positive neurons in formalin + MK-801 group significantly decreased in contrast to those of formalin 12 h group as well as the NO content (P < 0.01). CONCLUSION: Intrathecal injection of NMDA receptor antagonist MK-801 could inhibit the NOS activity and NO production in hippocampus of rat, which showed the increase of hippocampal NO production was mainly induced by the peripheral nociceptive information input. SN - 1000-6834 UR - https://www.unboundmedicine.com/medline/citation/21155253/[Intrathecal_injection_of_MK_801_inhibited_the_NOS_activity_and_NO_content_of_hippocampus_in_rat_during_the_process_of_formalin_induced_inflammatory_pain]_ L2 - https://medlineplus.gov/pain.html DB - PRIME DP - Unbound Medicine ER -