Tags

Type your tag names separated by a space and hit enter

Anthelmintic activity of botanical extracts against sheep gastrointestinal nematodes, Haemonchus contortus.
Parasitol Res. 2011 Jul; 109(1):37-45.PR

Abstract

The source of chemical anthelmintics (levamisole, flubendazole, and thiabendazole) had limited the success of gastrointestinal nematodiasis control in sheep and goats and thus awakened interest in the study of medicinal plant extracts as alternative sources of anthelmintics. The egg hatching and larvicidal effect of indigenous plant extracts were investigated against the sheep parasite, Haemonchus contortus. The purpose of the present study was to assess the efficacy of leaf, bark, and seed ethyl acetate, acetone and methanol extracts of Andrographis paniculata (Burm.f.) Wall. ex Nees., Anisomeles malabarica (L.) R. Br., Annona squamosa L., Datura metel L., and Solanum torvum Swartz were tested against the parasitic nematode of small ruminants H. contortus using egg hatch assay (EHA) and larval development assay (LDA). The assays were run in 24-well cell culture plates at room temperature with five replicates. All plant extracts showed moderate parasitic effects after 48 and exposure for egg hatching and LDA, respectively; however, 100% egg hatching and larvicidal inhibition were found in the methanol extracts of A. paniculata, A. squamosa, D. metel, and S. torvum at 25 mg/ml and the effect was similar to positive control of Albendazole (0.075 mg/ml) and Ivermectin (0.025mg/ml) against H. contortus, respectively. The EHA result showed the ED(50) of methanol extracts of A. paniculata and D. metel, which were 2.90 and 3.08 mg/ml, and in larval development assay, the ED(50) was 4.26 and 3.86 mg/ml, respectively. These effects remain to be confirmed through in vivo studies.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Kamaraj C
Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, 632 509 Vellore, Tamil Nadu, India.
Rahuman AA
No affiliation info available
Elango G
No affiliation info available
Bagavan A
No affiliation info available
Zahir AA
No affiliation info available

MeSH

AnimalsAnthelminticsFernsHaemonchusPlant BarkPlant ExtractsPlant LeavesSeedsSurvival Analysis

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21161270

Citation

Kamaraj, Chinnaperumal, et al. "Anthelmintic Activity of Botanical Extracts Against Sheep Gastrointestinal Nematodes, Haemonchus Contortus." Parasitology Research, vol. 109, no. 1, 2011, pp. 37-45.
Kamaraj C, Rahuman AA, Elango G, et al. Anthelmintic activity of botanical extracts against sheep gastrointestinal nematodes, Haemonchus contortus. Parasitol Res. 2011;109(1):37-45.
Kamaraj, C., Rahuman, A. A., Elango, G., Bagavan, A., & Zahir, A. A. (2011). Anthelmintic activity of botanical extracts against sheep gastrointestinal nematodes, Haemonchus contortus. Parasitology Research, 109(1), 37-45. https://doi.org/10.1007/s00436-010-2218-y
Kamaraj C, et al. Anthelmintic Activity of Botanical Extracts Against Sheep Gastrointestinal Nematodes, Haemonchus Contortus. Parasitol Res. 2011;109(1):37-45. PubMed PMID: 21161270.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anthelmintic activity of botanical extracts against sheep gastrointestinal nematodes, Haemonchus contortus. AU - Kamaraj,Chinnaperumal, AU - Rahuman,Abdul Abdul, AU - Elango,Gandhi, AU - Bagavan,Asokan, AU - Zahir,Abdul Abduz, Y1 - 2010/12/14/ PY - 2010/11/13/received PY - 2010/12/02/accepted PY - 2010/12/17/entrez PY - 2010/12/17/pubmed PY - 2011/10/5/medline SP - 37 EP - 45 JF - Parasitology research JO - Parasitol Res VL - 109 IS - 1 N2 - The source of chemical anthelmintics (levamisole, flubendazole, and thiabendazole) had limited the success of gastrointestinal nematodiasis control in sheep and goats and thus awakened interest in the study of medicinal plant extracts as alternative sources of anthelmintics. The egg hatching and larvicidal effect of indigenous plant extracts were investigated against the sheep parasite, Haemonchus contortus. The purpose of the present study was to assess the efficacy of leaf, bark, and seed ethyl acetate, acetone and methanol extracts of Andrographis paniculata (Burm.f.) Wall. ex Nees., Anisomeles malabarica (L.) R. Br., Annona squamosa L., Datura metel L., and Solanum torvum Swartz were tested against the parasitic nematode of small ruminants H. contortus using egg hatch assay (EHA) and larval development assay (LDA). The assays were run in 24-well cell culture plates at room temperature with five replicates. All plant extracts showed moderate parasitic effects after 48 and exposure for egg hatching and LDA, respectively; however, 100% egg hatching and larvicidal inhibition were found in the methanol extracts of A. paniculata, A. squamosa, D. metel, and S. torvum at 25 mg/ml and the effect was similar to positive control of Albendazole (0.075 mg/ml) and Ivermectin (0.025mg/ml) against H. contortus, respectively. The EHA result showed the ED(50) of methanol extracts of A. paniculata and D. metel, which were 2.90 and 3.08 mg/ml, and in larval development assay, the ED(50) was 4.26 and 3.86 mg/ml, respectively. These effects remain to be confirmed through in vivo studies. SN - 1432-1955 UR - https://www.unboundmedicine.com/medline/citation/21161270/Anthelmintic_activity_of_botanical_extracts_against_sheep_gastrointestinal_nematodes_Haemonchus_contortus_ L2 - https://dx.doi.org/10.1007/s00436-010-2218-y DB - PRIME DP - Unbound Medicine ER -