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[Effects of ginkgo biloba extracts on NMDA-activated currents in acutely isolated rat hippocampal neurons].
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2007 Aug; 23(3):305-8.ZY

Abstract

AIM

To investigate effect of ginkgo biloba extract (GBE) on N-methyl-D-aspartate (NMDA)-activated currents (I(NMDA)) and evaluate further the modulatory effects of Micro-GBE/Nano-GBE.

METHODS

By means of whole-cell patch clamp technique, NMDA-activated currents from acutely isolated rat hippocampal neurons were recored.

RESULTS

The majority of the neurons examined (81.8%, 90/110) were sensitive to NMDA (1 mmol/L) and its co-agonist Gly (10 micromol/L). NMDA activated an inward current, which manifested apparent desensitization and could be blocked by its specific antagonist MK-801. After the neurons were treated with Micro/Nano GBE (0.1 mg/ml) followed by the application of NMDA (1 mmol/L) and Gly (10 micromol/L) for 30 s, it was show that NMDA-activated currents were obviously inhibited (P < 0.01, n = 8). The inhibitory rate were 40% +/- 17% and 64% +/- 15% respectively. It showed that the modulatory effect of Nano-GBE (dissolved in the stander extracellular solution) on NMDA-activated current was significantly higher than that of Micro-GBE (dissolved in DMSO) (P < 0.05).

CONCLUSION

The inward currents activated by NMDA could be depressed by Micro-GBE and Nano-GBE. The modulatory effects of GBE on NMDA-activated current are expected to contribute to the neuroprotective effects of ginkgo biloba extracts. In addition, at the same concentration, the modulatory effect of Nano-GBE on NMDA-activated current is better than that of Micro-GBE.

Authors+Show Affiliations

Department of Physiology and Institute for Brain Disorder and Disease, Dalian Medical University, Dalian 116027, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

21162271

Citation

Li, Shao, et al. "[Effects of Ginkgo Biloba Extracts On NMDA-activated Currents in Acutely Isolated Rat Hippocampal Neurons]." Zhongguo Ying Yong Sheng Li Xue Za Zhi = Zhongguo Yingyong Shenglixue Zazhi = Chinese Journal of Applied Physiology, vol. 23, no. 3, 2007, pp. 305-8.
Li S, Sun CK, Ji XC, et al. [Effects of ginkgo biloba extracts on NMDA-activated currents in acutely isolated rat hippocampal neurons]. Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2007;23(3):305-8.
Li, S., Sun, C. K., Ji, X. C., & Zhang, J. (2007). [Effects of ginkgo biloba extracts on NMDA-activated currents in acutely isolated rat hippocampal neurons]. Zhongguo Ying Yong Sheng Li Xue Za Zhi = Zhongguo Yingyong Shenglixue Zazhi = Chinese Journal of Applied Physiology, 23(3), 305-8.
Li S, et al. [Effects of Ginkgo Biloba Extracts On NMDA-activated Currents in Acutely Isolated Rat Hippocampal Neurons]. Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2007;23(3):305-8. PubMed PMID: 21162271.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of ginkgo biloba extracts on NMDA-activated currents in acutely isolated rat hippocampal neurons]. AU - Li,Shao, AU - Sun,Chang-Kai, AU - Ji,Xin-Cai, AU - Zhang,Jian, PY - 2010/12/18/entrez PY - 2007/8/1/pubmed PY - 2012/3/27/medline SP - 305 EP - 8 JF - Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology JO - Zhongguo Ying Yong Sheng Li Xue Za Zhi VL - 23 IS - 3 N2 - AIM: To investigate effect of ginkgo biloba extract (GBE) on N-methyl-D-aspartate (NMDA)-activated currents (I(NMDA)) and evaluate further the modulatory effects of Micro-GBE/Nano-GBE. METHODS: By means of whole-cell patch clamp technique, NMDA-activated currents from acutely isolated rat hippocampal neurons were recored. RESULTS: The majority of the neurons examined (81.8%, 90/110) were sensitive to NMDA (1 mmol/L) and its co-agonist Gly (10 micromol/L). NMDA activated an inward current, which manifested apparent desensitization and could be blocked by its specific antagonist MK-801. After the neurons were treated with Micro/Nano GBE (0.1 mg/ml) followed by the application of NMDA (1 mmol/L) and Gly (10 micromol/L) for 30 s, it was show that NMDA-activated currents were obviously inhibited (P < 0.01, n = 8). The inhibitory rate were 40% +/- 17% and 64% +/- 15% respectively. It showed that the modulatory effect of Nano-GBE (dissolved in the stander extracellular solution) on NMDA-activated current was significantly higher than that of Micro-GBE (dissolved in DMSO) (P < 0.05). CONCLUSION: The inward currents activated by NMDA could be depressed by Micro-GBE and Nano-GBE. The modulatory effects of GBE on NMDA-activated current are expected to contribute to the neuroprotective effects of ginkgo biloba extracts. In addition, at the same concentration, the modulatory effect of Nano-GBE on NMDA-activated current is better than that of Micro-GBE. SN - 1000-6834 UR - https://www.unboundmedicine.com/medline/citation/21162271/[Effects_of_ginkgo_biloba_extracts_on_NMDA_activated_currents_in_acutely_isolated_rat_hippocampal_neurons]_ DB - PRIME DP - Unbound Medicine ER -